EFFECTS OF MERCURIC CHLORIDE ON MALE REPRODUCTION

氯化汞对男性生殖的影响

• 批准号: 7561453
• 负责人: HARI Om GOYAL
• 金额: $ 9.57万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-01 至 2008-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7561453
• 关键词: Affect; Age; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Development; Environment; Female; Fertility Rates; Fetus; Funding; Grant; Ingestion; Institution; Mercuric chloride; Mercury; Numbers; Ovulation; Ovum; Partner in relationship; Pregnancy; Rattus; Reproduction; Research; Research Personnel; Resources; Side; Source; Staging; United States National Institutes of Health; Uterus; Water; Weight Gain; assault; corpus luteum; day; implantation; male

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study was to investigate effects of mercuric chloride (MC) ingestion on the fertility rate of female rats including (1) the number of viable fetuses, (2) the number of implantations, and (3) the number of corpora lutea. Female rats, 102 days of age, were treated with 2.5 mg/kg/day of MC (treatment group) or de-ionized water (control group) for a period of 60 days. They were then mated with 70 to 80-day-old untreated males (2 females to 1 male) for 17 days. Pregnancy was confirmed by weight increase. Upon confirmation of pregnancy, all females were euthanized and examined for the number of viable fetuses, implantations, and corpora lutea in both sides. The mercury treatment resulted in lowering the mean number of fetuses (6.9 vs 12.4 in controls) and implantations (10.5 vs 12.8 in controls); however, the decrease was significant (P less than 0.05) only in the case of fetuses. Conversely, the number of corpora lutea was not different between the treated and control groups (14.9 vs 14.8 in controls). Hence, it is hypothesized that the mercury-induced reduction in the number of fetuses resulted at a post-implantation stage; probably from a malfunction of the uterus so that it could not provide a proper environment for the development of the fetus, or from a direct assault of mercury on the fetus, or both. It is further hypothesized that mercury ingestion did not affect ovulation since the number of corpora lutea (indicator for the number of ova released) was not different between the treated and control groups.

该副本是利用众多研究子项目之一 由NIH/NCRR资助的中心赠款提供的资源。子弹和 调查员（PI）可能已经从其他NIH来源获得了主要资金， 因此可以在其他清晰的条目中代表。列出的机构是 对于中心，这不一定是调查员的机构。 这项研究的目的是研究摄入汞（MC）对雌性大鼠的生育率的影响，包括（1）可行的胎儿数量，（2）植入数量，以及（3）lutea的数量。 雌性大鼠为102天，用2.5 mg/kg/天的MC（治疗组）或去离子水（对照组）治疗60天。然后，将他们与70至80天的未经治疗的雄性（2至1名男性）交配17天。体重增加证实了怀孕。确认怀孕后，对所有女性进行了安乐死，并检查了双方可行的胎儿，植入和cor虫的数量。 汞处理导致降低胎儿的平均数量（对照组为6.9 vs 12.4）和植入（对照中的10.5 vs 12.8）；然而，仅在胎儿的情况下，减少显着（P小于0.05）。相反，治疗组和对照组之间的lutea数量没有差异（对照组为14.8）。 因此，假设汞引起的胎儿数量减少在植入后阶段。可能是由于子宫的故障，因此无法为胎儿的发展提供适当的环境，或者是直接攻击胎儿或两者兼而有之。 进一步假设，由于治疗组和对照组之间的汞数量（释放的OVA数量指标）没有差异，因此汞摄入不会影响排卵。