EFFECTS OF MERCURIC CHLORIDE ON MALE REPRODUCTION

氯化汞对男性生殖的影响

• 批准号: 7959273
• 负责人: HARI Om GOYAL
• 金额: $ 4.89万
• 依托单位: TUSKEGEE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2009
• 资助国家: 美国
• 起止时间: 2009-09-18 至 2010-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7959273
• 关键词: Affect; Age; Area; Biomedical Research; Blood - brain barrier anatomy; Child; Computer Retrieval of Information on Scientific Projects Database; Control Groups; Diet; Female; Fertility Rates; Fetus; Fishes; Funding; Grant; Ingestion; Institution; Life; Mercuric chloride; Mercury; Partner in relationship; Pregnancy; Progress Reports; Rattus; Reporting; Reproduction; Research; Research Personnel; Resources; Source; United States National Institutes of Health; Weight Gain; Woman; corpus luteum; drinking water; female reproductive system; implantation; male; young adult

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The objective of this study was to investigate effects of organic methyl mercury (MeHg) ingestion on the fertility rate of female rats, including (1) the number of viable fetuses, (2) the number of implantations, and the number of corpora lutea. Female rats, 30 days of age, were treated with 2.5 ppm (low treatment group) or 5.0 ppm (high treatment group) or 0.0 ppm MeHg (control group), in drinking water, for 60 days. They were then mated with 70 to 80-day-old untreated males (2 females to 1 male) for 17 days. Pregnancy was confirmed by weight increase. At 12-15 days of gestation, all females were euthanized and examined for the number of viable fetuses and implantations. Compared to controls, neither the number of viable fetuses, nor the number of implantations, and nor the number of corpora lutea was altered, as a result of MeHg treatment. Conversely, as reported previously in 2006-07 progress report, the ingestion of inorganic mercury resulted in lowering the mean number of fetuses (6.9 vs 12.4 in controls) as well as implantations (10.5 vs 12.8 in controls). In conclusion, while MeHg does not adversely affect the female reproductive system, inorganic mercury does. The difference between the two may lie in differences in their ability to cross the blood-brain-barrier, which is crossed by the MeHg but not by the inorganic mercury. These results are significant because they have a direct impact on young and adult women, especially child-bearing women, who live near coastal areas and depend upon fish as their main source of diet.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 本研究的目的是调查有机甲基汞（MeHg）摄入对雌性大鼠生育率的影响，包括（1）可存活胎儿的数量，（2）植入数量和黄体数量。 30 天龄的雌性大鼠用 2.5 ppm（低处理组）或 5.0 ppm（高处理组）或 0.0 ppm MeHg（对照组）饮用水处理 60 天。然后将它们与 70 至 80 天大的未经处理的雄性（2 雌性对 1 雄性）交配 17 天。通过体重增加证实怀孕。妊娠 12-15 天时，对所有雌性实施安乐死，并检查存活胎儿和植入的数量。 与对照组相比，甲基汞治疗的结果是，存活胎儿的数量、着床数量和黄体数量都没有改变。 相反，正如之前在 2006-07 年进度报告中所报道的，摄入无机汞会导致平均胎儿数量（对照组为 6.9 个 vs 12.4 个）以及着床数量（对照组为 10.5 个 vs 12.8 个）降低。 总之，虽然甲基汞不会对女性生殖系统产生不利影响，但无机汞却会产生不利影响。 两者之间的差异可能在于它们穿过血脑屏障的能力不同，甲基汞可以穿过血脑屏障，但无机汞则不能穿过血脑屏障。这些结果意义重大，因为它们对年轻和成年妇女，特别是育龄妇女产生直接影响，她们生活在沿海地区附近，以鱼类为主要饮食来源。