Biosynthesis of deazapurine-containing metabolites

含脱氮嘌呤代谢物的生物合成

• 批准号: 7177512
• 负责人: VAHE BANDARIAN
• 金额: $ 23.47万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-01-17 至 2009-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7177512
• 关键词: Address; Anabolism; Antibiotics; Biochemical; Cells; Chemicals; Chemistry; Civilization; Cloning; Commit; Cosmids; DNA Library; Enzymatic Biochemistry; Enzymes; GTP Cyclohydrolase II; Genes; Genome; Genomics; Goals; Guanosine Triphosphate; Homologous Gene; Hormones; Housing; Human; In Vitro; Individual; Ions; Libraries; Life; Literature; Metabolism; Metals; Molecular Biology; Open Reading Frames; Organism; Pathway interactions; Play; Process; Production; Protein Biochemistry; Protein Overexpression; Proteins; Purines; Radiolabeled; Research; Riboflavin; Route; Streptomyces; Substrate Specificity; System; Testing; Therapeutic Agents; antitumor agent; enzyme activity; feeding; genome sequencing; human disease; interest; knowledge base; purine; radiotracer; reconstitution; research study; tool; vector

DESCRIPTION (provided by applicant): All organisms produce a host of compounds that are generally not required in primary metabolic processes, but play important regulatory functions. These secondary metabolites have been exploited by every civilization to enhance life and cure human diseases. We know these as hormones, antibiotics, antitumor agents, and antivirals. Since these metabolites can harm cells if produced in excess, or if present when a need for them does not exist, their production is often strictly regulated. An understanding of how cells synthesize and regulate the production of secondary metabolites is essential if one is to be able to exploit them as human therapeutic agents. Deazapurines are secondary metabolites that are derived from purines and little is known about how they are synthesized in the cell. This proposal outlines studies on the biosynthesis of deazapurine-containing secondary metabolites by Streptomyces. Our goal is to bring to fore the tools of genomics, molecular biology and enzymology to form the framework of how these metabolites are synthesized, and to begin the process of exploring the chemistry that underlies the biosynthesis of these molecules.

描述（由申请人提供）：所有生物都会产生许多化合物，这些化合物通常在主要的代谢过程中不需要，而是发挥重要的调节功能。每个文明都利用了这些次要代谢产物，以改善生活并治愈人类疾病。我们知道这些是激素，抗生素，抗肿瘤剂和抗病毒药。由于这些代谢产物如果过量产生，或者在不存在需要时可能会损害细胞，则通常严格调节它们的产生。如果要能够将其作为人类治疗剂剥削，那么了解细胞如何合成和调节次级代谢产物的产生至关重要。脱氮嗪是次生代谢产物，这些代谢产物是从嘌呤中得出的，对它们在细胞中的合成方式知之甚少。该提议概述了链霉菌含二氮嗪的次生代谢产物的生物合成研究。我们的目标是提出基因组学，分子生物学和酶学的工具，以形成这些代谢物如何合成的框架，并开始探索基于这些分子生物合成的化学作用的过程。