RTE, a plant growth gene with a conserved role in metals

RTE，一种在金属中具有保守作用的植物生长基因

• 批准号: 7480534
• 负责人: CAREN CHANG
• 金额: $ 3.21万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7480534
• 关键词: ATP phosphohydrolase; Alleles; Animal Genetics; Animal Model; Animals; Arabidopsis; Binding; Biochemical; Biological Assay; Caenorhabditis elegans; Cellular biology; Copper; Ethylene; Ethylenes; Genes; Genetic; Health; Homeostasis; Homologous Gene; Human; Investigation; Ions; Label; Link; Localized; Mammalian Cell; Measures; Metals; Methods; Modeling; Molecular Chaperones; Molecular Genetics; Mouse-ear Cress; Organism; Pathway interactions; Pattern; Phenotype; Plant Model; Plants; Process; Proteins; Receptor Gene; Regulatory Pathway; Research Personnel; Role; Saccharomyces cerevisiae; Signal Transduction; Specificity; System; Testing; Transition Elements; Yeasts; base; copper-binding protein; genetic analysis; intracellular protein transport; mRNA Expression; mutant; novel; paralogous gene; plant growth/development; programs; protein localization location; receptor; receptor function; research study; sensor; tool; trafficking

The broad, long-term objective is to understand the mechanisms by which organisms control transition metal ions and the roles of these metals, particularly copper, in cell signaling and regulatory pathways. Copper homeostasis is of major importance to human health, yet the pathways for intracellular copper trafficking are unclear, and relatively few components in copper sensing and trafficking have been identified. This proposal focuses on RTE, a previously undescribed gene of novel sequence, which is hypothesized to encode a sensor, transporter or chaperone for copper. The RTE gene is highly conserved, and is present in a single copy in animals and in two copies in plants. The aim of this proposal is to define the role of RTE in metal homeostasis. The studies will center primarily on the RTE1 gene of the model plant Arabidopsis thaliana. Genetic analyses in Arabidopsis have linked RTE1 to two copper-binding proteins in ethylene signal transduction. One is the copper-requiring ethylene receptor ETR1, whose function is dependent on RTE1. The other is RAN1, a homolog of the human Menkes/Wilson's P-type ATPase copper transporter, which is likewise required for ethylene receptor function. Investigating the genetic and cellular basis for these connections to RTE1 in Arabidopsis will utilize powerful genetic tools for understanding the cellular roles of RTE. Parallel experiments with respect to RTE and the Menkes/Wilson's homolog CUA-1 will be performed in the animal genetic model Caenorhabditis elegans, allowing comparison and integration of results from an animal system with those in plants. Saccharomyces cerevisiae and mammalian cell culture will be employed for ethylene binding assays and copper binding assays, respectively. The proposed experiments will use a combination of molecular genetics, cell biology and biochemical methods. The elucidation of RTE function should have a direct impact on understanding the processes of metal homeostasis for the benefit of human health.

广泛的长期目标是了解生物控制过渡的机制 金属离子以及这些金属，尤其是铜在细胞信号传导和调节途径中的作用。 铜稳态对人类健康至关重要，但是细胞内铜的途径 贩运尚不清楚，铜感应和贩运的组成部分相对较少 确定。该提案的重点是RTE，RTE是一个以前未描述的新序列基因， 假设将传感器，转运蛋白或伴侣用于铜。 RTE基因高度高 保守，并在动物中的单个副本中存在，并在植物中的两个副本中存在。这个目的 提议是定义RTE在金属稳态中的作用。研究将主要集中于RTE1 模型植物拟南芥的基因。拟南芥中的遗传分析已将RTE1与两个联系在一起 乙烯信号转导中的铜结合蛋白。一个是征收铜的乙烯受体 ETR1，其功能取决于RTE1。另一个是RAN1，人类的同源物 Menkes/Wilson的P型ATPase铜转运蛋白，这也是乙烯受体所需的 功能。研究与拟南芥中与RTE1连接的遗传和细胞基础 利用强大的遗传工具来理解RTE的细胞作用。并行实验 尊重RTE和Menkes/Wilson的同源性CUA-1将在动物遗传模型中进行 秀丽隐杆线虫，可以比较和整合动物系统的结果 在植物中。酿酒酵母和哺乳动物细胞培养将用于乙烯结合 测定和铜绑定测定法。提出的实验将结合 分子遗传学，细胞生物学和生化方法。 RTE功能的阐明应具有 直接影响理解金属稳态的过程，以使人类健康受益。