RTE, a plant growth gene with a conserved role in metals

RTE，一种在金属中具有保守作用的植物生长基因

• 批准号: 6922979
• 负责人: CAREN CHANG
• 金额: $ 23.57万
• 依托单位: UNIV OF MARYLAND, COLLEGE PARK
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-01 至 2009-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6922979
• 关键词: Arabidopsis; Caenorhabditis elegans; Saccharomyces; biological signal transduction; copper; ethylenes; functional /structural genomics; gene induction /repression; gene interaction; metals; phenotype; plant genetics; spectrometry; tissue /cell culture; western blottings

DESCRIPTION (provided by applicant): The broad, long-term objective is to understand the mechanisms by which organisms control transition metal ions and the roles of these metals, particularly copper, in cell signaling and regulatory pathways. Copper homeostasis is of major importance to human health, yet the pathways for intracellular copper trafficking are unclear, and relatively few components in copper sensing and trafficking have been identified. This proposal focuses on RTE, a previously undescribed gene of novel sequence, which is hypothesized to encode a sensor, transporter or chaperone for copper. The RTE gene is highly conserved, and is present in a single copy in animals and in two copies in plants. The aim of this proposal is to define the role of RTE in metal homeostasis. The studies will center primarily on the RTE1 gene of the model plant Arabidopsis thaliana. Genetic analyses in Arabidopsis have linked RTE1 to two copper-binding proteins in ethylene signal transduction. One is the copper-requiring ethylene receptor ETR1, whose function is dependent on RTE1. The other is RAN1, a homolog of the human Menkes/Wilson's P-type ATPase copper transporter, which is likewise required for ethylene receptor function. Investigating the genetic and cellular basis for these connections to RTE1 in Arabidopsis will utilize powerful genetic tools for understanding the cellular roles of RTE. Parallel experiments with respect to RTE and the Menkes/Wilson's homolog CUA-1 will be performed in the animal genetic model Caenorhabditis elegans, allowing comparison and integration of results from an animal system with those in plants. Saccharomyces cerevisiae and mammalian cell culture will be employed for ethylene binding assays and copper binding assays, respectively. The proposed experiments will use a combination of molecular genetics, cell biology and biochemical methods. The elucidation of RTE function should have a direct impact on understanding the processes of metal homeostasis for the benefit of human health.

描述（由申请人提供）：广泛的长期目标是了解生物控制过渡金属离子以及这些金属（尤其是铜）在细胞信号传导和调节途径中的作用的机制。 铜稳态对人类健康至关重要，但是细胞内铜贩运的途径尚不清楚，并且已经确定了相对较少的铜感应和贩运成分。该提案的重点是RTE，RTE是一种以前未描述的新序列基因，该基因的编码是用铜编码传感器，转运蛋白或伴侣的。 RTE基因是高度保守的，并且存在于动物中的单个副本中，并在植物中的两个副本中存在。该提议的目的是定义RTE在金属稳态中的作用。这些研究将主要集中于模型植物拟南芥的RTE1基因。拟南芥中的遗传分析已将RTE1与乙烯信号转导中的两个铜结合蛋白联系起来。一种是铜制乙烯受体ETR1，其功能取决于RTE1。另一个是RAN1，是人类Menkes/Wilson的P型ATPase铜转运蛋白的同源物，这对于乙烯受体功能也是所需的。研究拟南芥中与RTE1连接的遗传和细胞基础将利用强大的遗传工具来理解RTE的细胞作用。关于RTE和Menkes/Wilson的同源性CUA-1的并行实验将在秀丽隐杆线虫Caenorhabditis中进行，从而可以比较和整合动物系统的结果与植物中的动物系统。酿酒酵母和哺乳动物细胞培养物将分别用于乙烯结合测定和铜结合测定法。提出的实验将结合分子遗传学，细胞生物学和生化方法的组合。 RTE功能的阐明应直接影响理解金属体内平衡的过程，以使人类健康受益。