DNA segregation during Bacillus growth and development

芽孢杆菌生长和发育过程中的 DNA 分离

• 批准号: 7492397
• 负责人: JOSEPH A POGLIANO
• 金额: $ 2.32万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-01 至 2010-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7492397
• 关键词: Actins; Affect; Animal Model; Antibiotic Resistance; Antibiotics; Bacillus (bacterium); Bacteria; Bacterial DNA; Behavior; Biological; Biology; Cells; Cellular biology; DNA; DNA Sequence; Development; Diffusion; Distant; Ensure; Evolution; Family; Filament; Future; Generations; Genes; Genetic; Goals; Gram-Negative Bacteria; Growth; Growth and Development function; Homologous Gene; In Vitro; Indigenous; Individual; Infection; Inherited; Molecular Genetics; Mothers; Mutation; Numbers; Organism; Pathogenesis; Pathogenicity; Plasmid Cloning Vector; Plasmids; Play; Principal Investigator; Process; Proteins; Relative (related person); Replicon; Reproduction spores; Role; Site-Directed Mutagenesis; Study models; System; Testing; Toxin; Tubulin; Virulence; Virulence Factors; Work; capsule; in vivo; microbial; novel; plasmid DNA; programs; research study; segregation; size; tool; trait

The goal of this proposal is to identify plasmid DNA partitioning systemsthat are active during both . vegetative growth and sporulation in two different species of Bacillus. New tools for elucidating the mechanisms underlying plasmid segregation will be developed using the model organism B. subtilis, and then applied to B. megaterium. Utilizing two different Bacillus strains to study this problem will allow us to take advantage of the unique strengths that each organism and its indigenous plasmids offer, while maintaining a focused goal of understanding how conservedelements of the microbial DNA segregation machinery functions during sporulation to ensure that every spore inherits at least one copy of the plasmid. Plasmid segregation during sporulation provides an attractive system for studying DNA segregation because: 1) both the plasmids and the sporulation process are not nonessential; 2) the nearly 10 fold smaller volume of the forespore comparedto the mothercell demands an efficient plasmid partitioning system; 3) cell-specifictranscription factorsbecome active during sporulation, allowing the development of . powerful genetic and cell biology assaysfor DNA segregation during sporulation; and 4) many Bacillus plasmids do not encode proteins related to either the ParA or ParM families of plasmid segregation proteins, suggesting that novel partitioning mechanisms are at work. Since a wide variety of virulence factors are encoded on plasmids in pathogenic strains of Bacillus, understanding how plasmids are transmitted from one generation to the next is important for understanding the evolution and spread these key virulence determinants. These studies will allow the development of better genetic tools and plasmid vectors useful for manipulating many Bacillus species.

该提案的目的是识别质粒DNA分配系统的活性。 两种不同种类的芽孢杆菌的营养生长和孢子形成。阐明的新工具 使用模型有机体B.枯草酵母和 然后申请到B. Megaterium。利用两种不同的芽孢杆菌菌株研究此问题将使我们能够 利用每个生物体及其土著质粒提供的独特优势，而 保持集中目标，以了解微生物DNA隔离的保守元素 机械在孢子体过程中起作用，以确保每个孢子至少继承质粒的至少一个副本。 孢子形成过程中的质粒分离提供了一个有吸引力的系统来研究DNA分离 因为：1）质粒和孢子形过程都不是不必要的； 2）将近10倍 比较到母细胞的较小体积的前孔需要有效的质粒分配 系统; 3）在孢子形成过程中活跃的细胞特异性转录因子，允许发育。 在孢子形成过程中，强大的遗传和细胞生物学测定了DNA分离； 4）许多芽孢杆菌 质粒不编码与质粒分离蛋白的PARA或PARM家族有关的蛋白质， 暗示新颖的分区机制在起作用。由于多种毒力因素是 在芽孢杆菌的致病性菌株中编码在质粒上，了解质粒是如何从一个传播的 下一个生成对于理解演变和传播这些关键毒力很重要 决定因素。这些研究将允许开发更好的遗传工具和质粒向量有用 操纵许多芽孢杆菌。