Rapid FISH Assay for Oncogenic Papillomavirus RNAs

致癌乳头瘤病毒 RNA 的快速 FISH 检测

• 批准号: 6783732
• 负责人: PHILLIP T MOEN
• 金额: $ 18万
• 依托单位: ONE CELL SYSTEMS, INC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2005-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6783732
• 关键词: Papillomavirus; cancer prevention; cervical /vaginal smear; cervix neoplasms; diagnosis design /evaluation; early diagnosis; female; fluorescent dye /probe; fluorescent in situ hybridization; genetic transcription; human tissue; neoplasm /cancer diagnosis; neoplasm /cancer genetics; neoplastic cell; nucleic acid probes; nucleic acid quantitation /detection; oligonucleotides; oncogenes; oncogenic virus; virus RNA; virus related neoplasm /cancer; women' s health

DESCRIPTION (provided by applicant): Cervical cancer, the second most prevalent cancer of women worldwide, and the principal cancer of women in most developing countries, has been clearly linked to infection by human papillomavirus (HPV). In virtually all cervical cancer, HPV DNA is found integrated into the host genome, which results in the increased production of viral transforming proteins. In contrast to the morphologically-based traditional Pap test, molecular diagnostic methods detect HPV DNAs in cervical tissue, identifying whether a cancer-linked viral type is present. However, this diagnostic approach has not reduced morbidity and mortality, nor improved disease staging. Recent detection methods have focused on detection of HPV RNA using reverse transcriptase polymerase chain reaction. Although sensitive, no cytological examination of the cell sample is possible and the method continues to be plagued by procedural problems. In situ hybridization allows direct visualization of viral RNAs in addition to cytological examination; however, current in situ hybridization methods are time consuming, difficult to perform, and consist of many procedural steps. This Small Business Innovation Research project proposes to develop a rapid fluorescence in situ hybridization assay for detecting HPV RNAs.

描述（由申请人提供）：宫颈癌是全球第二大流行的女性癌症，以及大多数发展中国家的主要妇女癌症，与人类乳头瘤病毒（HPV）的感染显然与感染有关。在几乎所有宫颈癌中，发现HPV DNA都集成到宿主基因组中，从而导致病毒转化蛋白的产生增加。与基于形态学的传统子宫颈抹片检查相反，分子诊断方法检测宫颈组织中的HPV DNA，从而确定是否存在癌症连接的病毒类型。但是，这种诊断方法并未降低发病率和死亡率，也没有改善疾病分期。最近的检测方法专注于使用逆转录酶聚合酶链反应检测HPV RNA。尽管敏感，但对细胞样品的细胞学检查不可能，并且该方法继续受到程序问题的困扰。原位杂交还可以直接可视化病毒RNA，除了细胞学检查外；但是，当前的原位杂交方法是耗时的，难以执行的，并且由许多程序步骤组成。这个小型企业创新研究项目建议开发快速的荧光原位杂交测定法，以检测HPV RNA。