HSV REGULATION BY PROTEIN MODIFICATION

通过蛋白质修饰调节 HSV

• 批准号: 6706929
• 负责人: John A. Blaho
• 金额: $ 33.9万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-09-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6706929
• 关键词: herpes simplex virus 1; host organism interaction; phosphorylation; posttranslational modifications; protein structure function; protein transport; tissue /cell culture; virus infection mechanism; virus protein; virus replication

DESCRIPTION (provided by applicant): Human herpes simplex viruses (HSV-1 and HSV-2) cause significant morbidity and mortality in neonatal and immunocompromised populations. HSVs are cytolytic viruses which have profound impacts on their host cells. The broad, long-term objective of our research program is to understand the regulation of HSV infection in human cells. This research plan focuses on two representative, highly modified HSV proteins. The hypothesis being tested is that viral protein modification acts as a means to regulate HSV replication. The goal of the first specific aim is to use a combination of molecular genetics and cell biology techniques to determine the function of modified, nuclear VP22 during HSV infection. In the second specific aim, the goal is to combine genetic and physical biochemical analyses to correlate ICP22 posttranslational modifications with its function as a regulator of HSV replication. These studies will determine whether viral protein modifications are necessary processes in the replication of HSV. Since the focus is on key features of virus-host interactions, our findings are of importance to studies of other related human viruses. In the long term, this research will help define the molecular basis of regulation of the life cycle of these important human pathogens.

描述（由申请人提供）：人类单纯疱疹病毒（HSV-1和 HSV-2）在新生儿和 免疫功能低下的人群。 HSV是具有深刻的胞溶病毒 影响其宿主细胞。我们研究的广泛，长期目标 程序是了解人类细胞中HSV感染的调节。这 研究计划的重点是两种代表性，高度改良的HSV蛋白。这 测试的假设是病毒蛋白修饰是 调节HSV复制。第一个特定目的的目标是使用 分子遗传学和细胞生物学技术的结合以确定 HSV感染期间修饰的核VP22的功能。在第二个特定 目的，目标是将遗传和物理生化分析结合到 将ICP22翻译后修饰与其功能作为一个 HSV复制的调节剂。这些研究将确定病毒是否 蛋白质修饰是复制HSV的必要过程。自从 重点是病毒宿主相互作用的关键特征，我们的发现是 对其他相关人类病毒的研究的重要性。从长远来看，这个 研究将有助于定义生命周期调节的分子基础 这些重要的人类病原体。