VIRAL NUCLEOTIDYLATION AND AUTOIMMUNITY

病毒核苷酸化和自身免疫

• 批准号: 2446073
• 负责人: John A. Blaho
• 金额: $ 8.48万
• 依托单位: ICAHN SCHOOL OF MEDICINE AT MOUNT SINAI
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-09-30 至 2000-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2446073
• 关键词: 2'3' cyclic nucleotide phosphodiesterase; antiviral antibody; biomarker; genetic mapping; herpes simplex virus 1; herpes simplex virus 2; human tissue; immunoglobulin M; immunoprecipitation; multiple sclerosis; nervous system infection; protein sequence; virus protein

IgM antibody specific for 2'-3' cyclic nucleotide phosphodiesterase I (CNPI), a protein enriched in oligodendrocytes and myelin membranes, is found in the cerebrospinal fluid and serum of 75 percent of patients with multiple sclerosis (MS). This project will explore the response to CNPI as a unique marker and specific probe for persistent viral infection in MS. The project will test the hypothesis that epitopes on CNP1 are structurally related to nucleotidyl moieties on herpes virus proteins. The specific aims of the project are to (1) isolate CNPI epitopes and confirm CNPI modification, (2) examine anti-CNPI IgM from MS sera for reactivity with known herpes virus proteins, and (3) use anti-CNPI IgM to isolate viral proteins from the brain of MS patients.

2'-3'环核苷酸磷酸二酯酶特异的IgM抗体 我（CNPI），一种富含少突胶质细胞和髓磷脂的蛋白质 膜，在脑脊液中发现，血清为75 多发性硬化症患者百分比（MS）。 这个项目将 探索对CNPI作为独特标记和特定的反应 MS中持续性病毒感染的探针。 该项目将测试 假设CNP1上的表位与 疱疹病毒蛋白上的核苷基部分。 具体目的 该项目是（1）隔离CNPI表位并确认CNPI 修改，（2）检查MS Sera的抗CNPI IgM的抗CNPI IgM 与已知疱疹病毒蛋白的反应性，（3）使用抗CNPI IgM从MS患者的大脑中分离病毒蛋白。