CORE-- MORPHOLOGY

核心——形态学

• 批准号: 6868897
• 负责人: MARK F JACQUIN
• 金额: $ 3.07万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6868897
• 关键词: biomedical facility; electron microscopy; histology; morphology; stainings

This CORE will continue to have as its general aim to facilitate and ensure the accomplishment of the individual projects' Specific Aims. In addition, the Core will provide centralized facilities at Washington University for staining neurons, ultrastructural analyses, and distribution of experimental animals that are used in more than one project.. As such, the CORE will: 1) make technical expertise available to all of the component projects, 2) make optimum use of individual experimental animals, 3) ensure that the data obtained from the different laboratories are comparable and analyzed in a like manner so as to circumvent common pitfalls associated with the correlation of findings obtained by independent observers and different analytic techniques, 4) administer the various projects, and 5) provide means for experimental and conceptual integration and external review. To accomplish these Specific Aims, this CORE will carry out 5 different functions. These are: 1. A single facility in St. Louis for staining the long-range projections and dendrites of somatosensory neurons in various experimental preparations- The CORE will operate a facility in St. Louis that will be used by Project #s1, 2, 4, and 5 to stain the processes of single neurons at various levels of somatosensory neuraxis. 2. A single electron microscopy facility in St. Louis for tissue processing and documentation of ultrastructure- Two projects in St. Louis (#s 1 and 4) require electron microscopy. We proposed to continue with Mr. Joseph DeMaro as the CORE EM technician and associated in place equipment to provide an avenue for study of central ultrastructural development under conditions of neurotrophin augmentation and stem cell transplantation. 3. Timely transmission of data and ideas between the different laboratories-The projects comprising this proposal are closely interrelated technically and conceptually. Scheduled bimonthly meetings will ensure that necessary information, materials and critical reviews are transmitted between laboratories on a regular basis. 4. Continual external review of progress and results-This aim will continue to be met by establishing new and appropriate internal and external advisory groups that will meet annually to evaluate our work. Experts in the field of neuronal development will also be invited to give presentations and visit less formally with project investigators. 5. Administration All requisite and personnel are currently functioning without our Program Project.

该核心将继续将其作为促进和确保各个项目特定目标的一般目标。此外，核心将在华盛顿大学提供集中的设施，用于染色神经元，超微结构分析以及在多个项目中使用的实验动物的分布。因此，核心将为所有组成项目提供技术专业知识，2）与单个实验动物的最佳使用，3）与不同的实验性相关的数据相关的数据相关的数据相关的数据，与分析相关联，与分析相关联，与分析相关联，与分析相关联，与分析相关联，与分析相关联。独立观察者和不同的分析技术获得的发现的相关性，4）管理各种项目，5）为实验和概念整合和外部审查提供了手段。为了实现这些特定目标，该核心将执行5个不同的功能。这些是：1。在各种实验制剂中染色体感神经元的长距离投影和树突的单一设施 - 核心将在圣路易斯运营一个设施，该设施将由项目＃S1、2、2、4和5的项目使用，以染色各种神经元在各个层次的神经元的过程中的过程。 2。圣路易斯的单个电子显微镜设施，用于组织加工和超微结构的文献 - 圣路易斯的两个项目（＃S 1和4）需要电子显微镜。我们提议继续以约瑟夫·德马罗（Joseph DeMaro）为核心技术员，并与设备相关联，以提供研究中央超微结构发展的途径，在神经营养蛋白增强和干细胞移植条件下。 3。在不同的实验室之间及时传输数据和思想 - 包括该建议的项目在技术上和概念上都密切相互关联。预定的每两个月会议将确保定期在实验室之间进行必要的信息，材料和关键评论。 4.对进步和结果的持续外部审查 - 将通过建立新的，适当的内部和外部咨询小组每年满足以评估我们的工作，继续实现此目标。还将邀请神经元发展领域的专家进行演讲，并与项目调查人员正式访问。 5。管理所有必要的和人员目前在没有我们的计划项目的情况下运作。