Malvern MicroCal PEAQ Isothermal Titration Calorimeter

Malvern MicroCal PEAQ 等温滴定量热仪

基本信息

批准号：
9274599
负责人：
Krishna Rajarathnam
金额：
$ 12.04万
依托单位：
UNIVERSITY OF TEXAS MED BR GALVESTON
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-04-15 至 2018-04-14
项目状态：
已结题

项目摘要

ABSTRACT Funds are requested for the acquisition of a Malvern MicroCal PEAQ-ITC (Isothermal Titration Calorimeter). ITC data will allow characterizing the thermodynamic signatures of a number of proteins that bind diverse targets and mediate fundamental functions, from immune responses and synapses to virology. Therefore, this equipment will significantly enhance our understanding of the nuanced relationships between thermodynamics, structure, and function, and expedite development and design of more potent and specific drugs for a wide variety of diseases including microbial infections, autism, and schizophrenia. Our previous, now defunct, VP-ITC was extensively used (by over a dozen research groups) and provided not only fundamental knowledge, but was valuable in securing many investigator-initiated federal grants. We cannot overemphasize the importance of an ITC for the Sealy Center for Structural Biology and Molecular Biophysics (SCSB) core faculty and our other colleagues who are interested in the molecular mechanisms of their proteins of interest. The new instrument will be housed in the existing SCSB space at UTMB, and will be supported by the SCSB. A Ph.D.-level solution biophysics Facility Manager, also fully supported by the SCSB, will oversee the system. Scheduling of the instrument will be web-based, and any conflicts will be resolved through a steering committee. Approximately 10% of the available hours will be reserved for new and outside users, as well as for pilot studies, and in particular, priority will be given to new faculty investigators to facilitate their competition for NIH support. Further, we are confident that the new ITC will stimulate new collaborations among investigators in our University.

抽象的需要资金购买 Malvern MicroCal PEAQ-ITC（等温滴定仪）热量计）。 ITC 数据将允许表征许多物质的热力学特征结合不同靶标并介导免疫反应基本功能的蛋白质和病毒学的突触。因此，该设备将显着增强我们的理解热力学、结构和功能之间的微妙关系，并加快开发和设计针对多种疾病的更有效和更特异性的药物包括微生物感染、自闭症和精神分裂症。我们的前任 VP-ITC（现已解散）被广泛使用（被十几个研究小组），并且不仅提供了基本的知识，但对于获得许多研究者发起的联邦拨款很有价值。我们不能过分强调 ITC 对于 Sealy 结构生物学中心的重要性分子生物物理学（SCSB）核心教师和我们其他对此感兴趣的同事他们感兴趣的蛋白质的分子机制。新仪器将安装在 UTMB 现有 SCSB 空间，并将得到 SCSB 的支持。博士级解决方案生物物理学设施经理也得到 SCSB 的全力支持，将监督该系统。仪器的安排将基于网络，任何冲突都将通过指导委员会。大约 10% 的可用时间将预留给新的和外部用户以及试点研究，特别是新教师将被优先考虑调查人员促进他们争夺 NIH 支持的竞争。此外，我们有信心新的 ITC 将促进我们大学研究人员之间的新合作。