LIFE ASSURANCE GENES IN DROSPHILA

果蝇的生命保障基因

• 批准号: 6629815
• 负责人: SEYMOUR BENZER
• 金额: $ 34.27万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-04-01 至 2004-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6629815
• 关键词: Drosophilidae; age difference; aging; binding proteins; biochemical evolution; bioenergetics; biological signal transduction; calorimetry; electron microscopy; free radical scavengers; gene expression; gene targeting; genetic library; genetic mapping; histochemistry /cytochemistry; light microscopy; longevity; molecular cloning; mutant; northern blottings; nucleic acid probes; stimulus /response; stress; suppressor mutations

Toward the discovery of life assurance genes, we are performing a screen for extended lifespan in Drosophila melanogaster by single gene mutations. The mutant line methuselah consistently displays an approximately 40 percent increase in average lifespan. Interestingly, the mutant flies also show enhanced resistance to conditions of stress, including starvation, low humidity, and high temperature. Upon exposure to paraquat, a free radical generator, they survive longer than control flies. Excision, along with adjacent DNA, results in embryonic lethality in homozygotes, suggesting that the gene also plays an important role during development. The gene structure predicts a protein sequence containing seven hydrophobic regions with homology to various G-protein-coupled, seven-transmembrane-domain cell receptors. This finding suggests that Drosophila can use signal transduction pathways to modulate stress response and aging. This project is to analyze the mechanism involved in methuselah, and to undertake an expanded project for the isolation and analysis of additional life assurance genes.

为了发现生命保证基因，我们正在进行筛选 通过单基因延长果蝇的寿命 突变。 突变株系 methuselah 始终表现出 平均寿命延长约 40%。有趣的是， 突变果蝇还表现出对应激条件的更强的抵抗力， 包括饥饿、低湿度和高温。 曝光后 对于自由基生成剂百草枯来说，它们的存活时间比对照组长 苍蝇。 连同邻近的 DNA 一起切除，产生胚胎 纯合子的致死率，表明该基因还发挥着 发展过程中发挥重要作用。 基因结构预测 含有七个疏水区的蛋白质序列，与 各种 G 蛋白偶联七跨膜结构域细胞 受体。 这一发现表明果蝇可以利用信号 调节应激反应和衰老的转导途径。这 该项目旨在分析玛撒拉所涉及的机制，并 进行一个扩大的项目来分离和分析 额外的生命保证基因。