APPLIED GENOMICS IN CARDIOPULMONARY DISEASE

心肺疾病中的应用基因组学

• 批准号: 6659065
• 负责人: EDWARD F HAPONIK
• 金额: $ 100.83万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2004-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6659065
• 关键词: asthma; biomedical resource; cardiopulmonary disease; cardiovascular disorder diagnosis; chronic obstructive pulmonary disease; clinical research; cooperative study; cryopreservation; cystic fibrosis; data collection methodology /evaluation; data management; functional /structural genomics; gene expression; genetic polymorphism; heart transplantation; human genetic material tag; human subject; human tissue; linkage mapping; lung injury; lung transplantation; medical outreach /case finding; microarray technology; molecular biology information system; pulmonary fibrosis /granuloma; pulmonary hypertension; respiratory disorder diagnosis; tissue resource /registry; transplant rejection

The Clinical Core will serve as the coordinating center for patient recruitment, specimen procurement and data base management. It is the mission of this Core to identify and provide disease-specific candidate genes from patients with such clinical disorders as acute lung injury, COPD, cystic fibrosis, asthma, pulmonary hypertension, pulmonary fibrosis, ischemic heart failure and both lung and cardiac transplant rejection. This core will also be responsible for the establishment of a Cardiopulmonary Tissue Repository that will maintain an archive of cryopreserved tissues and maintain an accurate data base of patient demographic and clinical data for correlation with biological end points produced by cDNA microarray. Following patient consent and registration, tissue samples and peripheral blood are delivered to the Core laboratory. The freezing facility will also serve as a repository for cyropreserved human lung and cardiac specimens. The Core Investigators will meet regularly to review all diagnostic materials on each specimen procured including histochemical stains and biopsies in order to determine a precise diagnosis and relevant demographic and clinical data for entry into the Project's data base which will include critical variables for the analysis of the biological data obtained.

临床核心将作为患者招募、标本采购和数据库管理的协调中心。该核心的使命是从患有急性肺损伤、慢性阻塞性肺病、囊性纤维化、哮喘、肺动脉高压、肺纤维化、缺血性心力衰竭以及肺和心脏移植排斥等临床疾病的患者中识别和提供疾病特异性候选基因。 。该核心还将负责建立心肺组织存储库，该存储库将维护冷冻组织的档案，并维护患者人口统计和临床数据的准确数据库，以便与 cDNA 微阵列产生的生物终点相关联。在患者同意和登记后，组织样本和外周血被运送到核心实验室。该冷冻设施还将作为冷冻保存的人类肺和心脏标本的储存库。核心研究人员将定期开会，审查每个标本的所有诊断材料，包括组织化学染色和活检，以确定精确的诊断和相关的人口统计和临床数据，以输入项目的数据库，其中包括用于分析的关键变量获得的生物学数据。