EVALUATION OF REPORTED EMF EFFECTS ON CELL COMMUNICATION

评估报告的电磁场对细胞通信的影响

• 批准号: 2019284
• 负责人: GUY D GRIFFIN
• 金额: $ 8.91万
• 依托单位: LOCKHEED MARTIN ENERGY RESEARCH CORP
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-04-20 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2019284
• 关键词: cell cell interaction; electromagnetic radiation; gap junctions; magnetic field; membrane channels; radiobiology; tissue /cell culture

DESCRIPTION: The major objective of the proposed work is to exactly replicate studies reported by Benane et al. (1996) in which Clone 9 cells, treated with concentrations of chloral hydrate which partially inhibit gap junctional intercellular communication (GJIC), are exposed to power frequency, i.e. extremely-low-frequency (ELF), electromagnetic fields (EMFs). A further reduction in GJIC produced by the ELF-EMF was reported to be consistent with the Ion Parametric Resonance (IPR) model, proposed by Blackman et al. (1993), which predicts distinct magnetic field interactions with biological systems. Equipment and an expertise in all relevant experimental techniques is already in place to perform these replication studies at Oak Ridge National Laboratory (ORNL). If the same results are observed using an identical exposure system as that used by Blackman and co-workers, then the consistency of the effect will be examined under the constraints of a different exposure system and modifications of the experimental protocol. The dedicated magnetic field exposure system at ORNL, which is one of four regional facilities in the United States, will be used to repeat, and expand the replication studies. This exposure system was designed to provide carefully controlled conditions for cell culture exposures to magnetic fields. In addition, extensive experience in the principal investigator's laboratory with GJIC studies suggests possible improvements in the experimental techniques used to measure GJIC. Modifications in scrape-loading procedures and the use of electrophysiology measurements are proposed for these subsequent studies of cell-to-cell coupling. If the main objectives, outlined above, substantiate the results reported by Blackman and co-workers then emphasis will be shifted to experiments aimed at understanding the mechanisms involved.

描述：拟议工作的主要目标是准确地 重复 Benane 等人报告的研究。 (1996) 其中克隆 9 细胞， 用一定浓度的水合氯醛处理，部分抑制间隙 细胞间连接通讯 (GJIC)，暴露于电源 频率，即极低频 (ELF)、电磁场 （电动势）。 据报道，ELF-EMF 产生的 GJIC 进一步减少 与离子参数共振（IPR）模型一致，由 布莱克曼等人。 (1993)，预测了不同的磁场相互作用 与生物系统。 所有相关领域的设备和专业知识 实验技术已经到位来执行这些复制 在橡树岭国家实验室（ORNL）进行研究。 如果结果相同 观察到使用与布莱克曼使用的相同的曝光系统 同事，那么效果的一致性将在下面进行检查 不同曝光系统的限制和修改 实验方案。 专用磁场暴露系统 ORNL 是美国四个地区设施之一，将 用于重复和扩展复制研究。 这个曝光系统 旨在为细胞培养提供精心控制的条件 暴露于磁场。 此外，在以下领域拥有丰富的经验： 首席研究员实验室与 GJIC 研究表明有可能 用于测量 GJIC 的实验技术的改进。 刮擦加载程序的修改和电生理学的使用 建议对这些后续的细胞间研究进行测量 耦合。 如果上述主要目标证实了结果 布莱克曼和同事报道称，那么重点将转移到 旨在了解所涉及机制的实验。