RFA-GH-20-005, Conducting Integrated Infectious Disease and Public Health Research in Peru

RFA-GH-20-005，在秘鲁进行综合传染病和公共卫生研究

• 批准号: 10842492
• 负责人: Margaret N Kosek
• 金额: $ 10万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-30 至 2025-09-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10842492
• 关键词: RFA; GH; 20; 005; Conducting

PROJECT SUMMARY: The proposed application aims to overcome diagnostic barriers in the etiologic diagnosis of endemic and epidemic causes of acute febrile illness (AFI) by moving efficient state of the art diagnostics to an area of high endemicity. The long-term goal of this research is to understand the etiology of AFI across diverse contexts in South America and contribute to global strategies that improve etiology-specific disease estimates to inform public health practice and policy. The central hypothesis is that the technology transfer and implementation of improved diagnostics will enhance regional diagnostic capacity, epidemic responsiveness, and translation of research findings to practice maximizing the public health impact of research findings. The rationale for the research is that the documented feasibility of this approach will enable a full and appropriate evaluation for uptake by regional and national stakeholders. The goals of the research will be achieved through the execution of three specific aims: 1) Establish stable year round acute febrile illness surveillance using the TaqMan array card, which simultaneously detects over 30 pathogens of AFI across three epidemiologic contexts in Loreto, Peru; urban, riverine with extensive sylvatic exposure, and agricultural riverine context along the three country border (Peru, Brazil, and Colombia) in order to capture the diversity of pathogens causing AFI in Peru over a four year period; 2) Integrate this diagnostic platform into the regional outbreak response to determine the etiology of epidemics of acute febrile illness and validating improved storage and transport procedures; and 3) Develop and evaluate novel serodiagnostic chip to enable single platform serologic assessment and downstream point of care tests and focused diagnostics for pathogens of high prevalence and importance. The global control of emerging infectious diseases will be improved only as the areas that are not reached and included effectively in surveillance shrink and the proposed project attempts to implement a strategy to make all populations reachable to the improved diagnostics. The complexity of current diagnostic strategies is a critical barrier to the timely regional ascertainment of the etiology of most cases of acute febrile illnesses and this has important regional and global public health implications as undiagnosed AFI allows for the widespread dissemination of emerging diseases as effective disease control measures are based on accurate etiologic assessment. The plan is innovative because of the meaningful integration of highly select local researchers, educators, trainees, and policymakers. The implementation of the research strategy will be significant because it is expected to contribute to the accuracy of etiologic assessment of endemic and epidemic etiologies of AFI in an area of documented diversity of vector-borne and zoonotic agents and serve as a model for enabling local diagnostic and reporting capacity in South America.

项目摘要： 拟议的应用旨在克服特有和 通过将有效的最先进诊断状态移动到一个高度的领域，导致急性发热疾病（AFI）的流行病原因 流行性。这项研究的长期目标是了解跨不同背景的AFI的病因 南美并促进了改善病因特异性疾病估计以告知的全球策略 公共卫生实践和政策。中心假设是技术转移和实施 改进的诊断将提高区域诊断能力，流行性反应能力以及翻译 研究结果是为了实践最大化研究结果的公共卫生影响。理由 研究是，这种方法的可行性将使能够进行完整而适当的评估 地区和国家利益相关者的吸收。研究的目标将通过执行实现 在三个具体目标中：1）使用塔克曼阵列建立全年稳定的急性高热疾病监视 卡，在洛雷托的三个流行病学环境中同时检测到30多个病原体AFI， 秘鲁;城市，河流，具有广泛的Sylvatic暴露以及三个国家的农业河流环境 边境（秘鲁，巴西和哥伦比亚），以捕获秘鲁在秘鲁的病原体多样性 四年期； 2）将此诊断平台集成到区域爆发响应中，以确定 急性发热疾病流行病的病因，并验证了改进的存储和运输程序； 3） 开发和评估新型的血清诊断芯片，以实现单个平台的血清学评估和下游 护理点测试和重点诊断，以示较高的患病率和重要性。全球控制 新兴的传染病仅在未达到并有效包括的区域时得到改善 在监视缩水和拟议的项目中，试图实施一项策略以使所有人口 可及改进的诊断。当前诊断策略的复杂性是对 及时确定大多数急性发热疾病病例的病因，这很重要 未经诊断的AFI的区域和全球公共卫生影响允许广泛传播 作为有效疾病控制措施的新兴疾病基于准确的病因学评估。计划 由于高度精选的本地研究人员，教育者，学员和 决策者。研究策略的实施将是重要的，因为它有望做出贡献 为了对AFI的流行病和流行病学的病因学评估的准确性 向量传播和人畜共患剂的多样性，并作为实现本地诊断和报告的模型 南美的能力。