Penn Center for Multi-scale Molecular Mapping of the Female Reproductive System

宾夕法尼亚大学女性生殖系统多尺度分子图谱中心

• 批准号: 10822203
• 负责人: JUNHYONG KIM
• 金额: $ 18.89万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-24 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10822203
• 关键词: 3-Dimensional; Anatomic Models; Anatomy; Atlases; Basic Science; Biological Assay; Cell Communication; Cells; Cellular biology; Characteristics; Chromatin; Clinical; Complex; Data; Deposition; Discipline of obstetrics; Fertility; Goals; Gynecology; Health; Human BioMolecular Atlas Program; Human Biology; Individual; Informatics; Location; Mammalian Oviducts; Maps; Metadata; Molecular; Operative Surgical Procedures; Organ; Ovary; Physiological; Procedures; RNA; Records; Reproductive Health; Reproductive Medicine; Research Personnel; Resources; Sampling; System; Therapeutic; Tissue Sample; Tissues; Uterus; Women' s Health; cell community; female reproductive system; human female; indexing; individual variation; molecular scale; multimodality; programs; single-cell RNA sequencing; structured data; transcriptomics

The female reproductive system, the uterus, fallopian tubes and the ovaries, is a complex interrelated set of organs that is physiologically dynamic and not only important for fertility but critically interrelated with general health. Single cell studies of the human female reproductive system and related tissues have been previously studied, but, as of yet, a comprehensive program, aligned with the goals of the HuBMAP, to define a molecular map of the entire system, integrating multi-modal assays, spatial diversity, and individual variations has not been established. The Penn Department of Obstetrics and Gynecology performs approximately 3,500 surgical procedures annually, of which many procedures allow sampling of multiple organs and locations from the same subject under normal conditions. Here, we propose to leverage the sampling opportunities afforded by the Penn ObGyn group and the single cell biology expertise of Penn investigators to establish a Penn Center for Multi-scale Molecular Map of the Female Reproductive System. We will obtain a comprehensive molecular characterization of the female reproductive system using six different molecular assays for at least ~700 tissue samples in anatomically indexed samples, creating a key resource for both basic science and women’s health. The molecular assays include single cell RNAseq, clampFISH spatial transcriptomics, simultaneous single cell open chromatin and RNA assays, and spatial open chromatin assay, among others. We will also generate a 3D anatomical model to provide spatial coordinate for our molecular characterization. All assay data will be registered to our 3D anatomical map that will be integrated with the HIVE Common Coordinate Framework. All metadata from subject records, clinical procedures, molecular procedures, and informatics pipelines will be collected, curated, and deposited as structured data. All data, including an extensive set of metadata, will be made available as a public resource. The completion of this resource will impact reproductive medicine for women’s health and also inform basic biology of human cell communities.

女性生殖系统，子宫、输卵管和卵巢，是一个复杂且相互关联的系统 具有生理动态的器官，不仅对生育很重要，而且与生育能力密切相关 对人类女性生殖系统和相关组织的单细胞研究。 以前曾进行过研究，但到目前为止，还没有一个与目标一致的综合计划 HuBMAP，定义整个系统的分子图谱，集成多模式测定、空间分析 多样性和个体差异尚未确定。 妇科每年进行约 3,500 例外科手术，其中许多手术 允许在正常条件下对同一受试者的多个器官和位置进行采样。 我们建议利用宾夕法尼亚州妇产科小组和单一机构提供的采样机会 宾夕法尼亚大学研究人员的细胞生物学专业知识建立了宾夕法尼亚大学多尺度分子图谱中心 我们将获得女性生殖系统的全面分子特征。 女性生殖系统使用六种不同的分子检测方法对至少约 700 个组织样本进行检测 解剖学索引的样本，为基础科学和女性健康创造了关键资源。 分子分析包括单细胞 RNAseq、clampFISH 空间转录组学、同步单细胞分析 我们还将进行细胞开放染色质和 RNA 检测以及空间开放染色质检测等。 生成 3D 解剖模型，为我们的分子表征提供空间坐标。 检测数据将被记录到我们的 3D 解剖图上，该图将与 HIVE Common 集成 协调框架。来自受试者记录、临床程序、分子程序的所有元数据。 信息学管道将作为结构化数据进行收集、整理和存储。 一套广泛的元数据将作为公共资源提供。 资源将影响妇女健康的生殖医学，并为人类的基础生物学提供信息 细胞群落。