Engineering hydrophilic/amphiphilic Vitamin B6-based super antioxidant dendrimers for controlling chronic inflammation

工程设计亲水性/两亲性维生素 B6 超级抗氧化树枝状聚合物，用于控制慢性炎症

• 批准号: 10798901
• 负责人: Choon Young Lee
• 金额: $ 10万
• 依托单位: CENTRAL MICHIGAN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10798901
• 关键词: Acceleration; Administrative Supplement; Anti-Inflammatory Agents; Antioxidants; Architecture; Arthritis; Asthma; Atherosclerosis; Cells; Chronic; Communities; Data; Dendrimers; Diabetes Mellitus; Disease; Electrospray Ionization; Employment; Engineering; Free Radicals; Funding; Future; Generations; Grant; Inflammation; Malignant Neoplasms; Measures; Michigan; Molecular; Molecular Weight; Monitor; Neurodegenerative Disorders; Oxidative Stress; Prevention; Reaction; Reader; Research; Science; Students; Techniques; Testing; Thin Layer Chromatography; Time; Training; Universities; Vitamin B6; Work; amphiphilicity; cost; graduate school; hydrophilicity; instrument; ionization technique; mass spectrometer; protective effect; public health relevance; skills; undergraduate student

Project Summary Funds are requested to purchase a new Advion CMS Expression-L Electrospray Ionization (ESI) Mass Spectrometer (MS) with a Thin Layer Chromatography (TLC) reader, which will be installed in the lab 343A of the Dow Science building at Central Michigan University (CMU). Electrospray Ionization (ESI) Mass Spectrometer (MS) measures molecular weights (MW) of molecules. For dendrons/dendrimers synthesis, molecular weight is the most critical information for molecular identification because other analytical techniques, such as NMR, cannot readily distinguish between the target dendrimer and incomplete reaction intermediates and dendrimers in different generations. Electrospray ionization (ESI) is a soft ionization technique, causing little to no fragmentation, which is an important criterion for analysis of dendrons and dendrimers with high molecular weights. Without an ESI instrument, our current grant- funded research will be greatly impacted because MS data is required at each intermediate step to move towards the target molecule synthesis. Currently there is no reliable, well-functioning ESI MS in the entire CMU community. The TLC plate reader measures molecular weight of each spot on the TLC plate and help locate the target dendrons (dendrimers) rapidly, contributing to enhancing work efficiency and saving overall cost and time in purification. In summary, (i) ESI MS is the most important analytical instrument for monitoring synthesis reactions of dendrimers/dendrons. (ii) This supplementary funding will allow us to train undergraduate/graduate research students. We believe that procurement of a state-of-the-art analytical instrument (ESI MS spectrometer) with this Administrative Supplement will help accelerate the pace of the proposed research as well as help students acquire a cornerstone MS technique, which is a desirable skill for future employment and graduate school.

项目摘要 要求资金购买新的Adwion CMS表达式L电喷雾电离（ESI）质量 具有薄层色谱（TLC）读取器的光谱仪（MS），将安装在实验室中 密歇根大学中央大学（CMU）的DOW科学大楼的343A。电喷雾电离（ESI） 质谱仪（MS）测量分子的分子量（MW）。用于树枝状/树突聚合物 合成，分子量是分子鉴定的最关键信息，因为其他 分析技术（例如NMR）无法轻易区分目标树枝状大分子和 不完整的反应中间体和树枝状聚合物在不同的世代中。电喷雾电离（ESI）为 软电离技术，几乎没有碎裂，这是分析的重要标准 具有高分子量的树突和树枝状聚合物。没有ESI仪器，我们当前的赠款 - 资金研究将受到极大影响，因为每个中级步骤都需要MS数据才能移动 朝向靶分子合成。目前，整个过程中没有可靠的，功能良好的ESI MS CMU社区。 TLC读取器测量TLC板上每个位置的分子量，并有助于 迅速找到目标树突（树枝状聚合物），有助于提高工作效率并节省 整体成本和净化时间。总而言之，（i）ESI MS是最重要的分析仪器 用于监测树突聚合物/树突的合成反应。 （ii）此补充资金将使我们 培训本科/研究生研究专业的学生。我们认为，采购 使用此管理补充剂的分析仪器（ESI MS光谱仪）将有助于加速 拟议研究的步伐以及帮助学生获得基石MS技术，这是一个 未来就业和研究生院的理想技能。