Chronic Intermittent Hypoxia and Hyperalgesic Priming - Administrative Supplement

慢性间歇性缺氧和痛觉过敏启动 - 行政补充

• 批准号: 10844191
• 负责人: NATHANIEL Aaron JESKE
• 金额: $ 1.6万
• 依托单位: UNIVERSITY OF TEXAS HLTH SCIENCE CENTER
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-03-01 至 2028-02-29
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10844191
• 关键词: 18 year old; Abate; Administrative Supplement; Affect; Afferent Neurons; American; Anti-Inflammatory Agents; Applications Grants; Behavior; Behavioral; Biochemical; Biological Assay; Cell Separation; Chemicals; Chronic; Clinical; Clinical Research; Complement; Continuous Positive Airway Pressure; DNA; DNA Binding; Dangerousness; Densitometry; Development; Diagnosis; Environment; Extramural Activities; Film; Fixatives; Friends; Funding; Future; Gel; Genetic Screening; Health; Hypoxia; Image; Immune; In Vitro; Inflammatory; Knowledge; Laboratories; Life; Link; Location; Macrophage; Maintenance; Measures; Membrane; Mission; Modeling; Molecular Genetics; Mus; National Institute of Neurological Disorders and Stroke; Neuroimmune; Neuronal Plasticity; Neurons; Nociceptors; Obstructive Sleep Apnea; Oxidative Stress; Pain; Pathogenicity; Patients; Pattern; Peripheral; Persistent pain; Personal Satisfaction; Pharmacology Study; Pharmacotherapy; Phase; Phenotype; Physiological; Pilot Projects; Population; Proteins; Proteomics; Public Health; Pump; Quality of life; RNA; Recurrence; Reproducibility; Research; Rodent; Roentgen Rays; Role; Sampling; Scanning; Services; Signal Pathway; Sleep; Sleep Disorders; Sleep disturbances; System; Technology; Testing; Tissue Sample; Tissues; Transcript; Translating; United States National Institutes of Health; Up-Regulation; Visualization; Western Blotting; Work; X-Ray Film; chronic pain; clinical diagnosis; comorbidity; cost; digital; digital imaging; experimental study; health determinants; hypoxia inducible factor 1; image processing; imager; imaging system; improved; in vivo; in vivo imaging; inducible Cre; innovation; molecular marker; multidisciplinary; nanoGold; nanoparticle drug; new technology; nocturnal Hypoxemia; pain behavior; pain chronification; pain sensation; preclinical study; protein biomarkers; protein expression

Our current extramural funding, through NINDS, R01 NS128574, entitled Chronic Intermittent Hypoxia and Hyperalgesic Priming, incorporates multiple proteomic experiments that depend on the visualization and quantification of protein expression patterns across multiple in vitro and in vivo tissue samples. Furthermore, Western blotting experiments are utilized throughout the grant proposal to support key experiments that track peripheral neuron and macrophage protein marker expression profiles. Unfortunately, our main piece of machinery for processing and visualizing Western blot membranes has come under a state of irreversible disrepair, and we require immediate replacement to continue experiments proposed in the application. We currently house an 18-year-old MiniMedical Services X-ray developer and processor to visualize Western blot membranes. This developer requires monthly service to clean and replace reservoirs with environmentally- dangerous developer and fixative solutions to produce films that are then digitally scanned and quantified for band densitometry by NIH Image 1.62 to determine protein expression patterns. Several months ago, 2 of the three necessary reservoir pumps failed. The cost to replace the broken pumps are estimated by service technicians to cost more than a brand-new machine, thereby forcing us to discontinue its use in our laboratory for proteomic research. We have since solicited bids to determine how we might replace the broken developer with a chemiluminescence imager. We received several quotes from multiple companies and submit the attached quote for your consideration. The ThermoFisher Life Technologies iBright CL750 Digital Imaging System is an economical replacement for the outdated MiniMed X-ray developer. This imaging system allows for imaging of chemiluminescent Western blots, protein gels, and DNA gels, and supersedes the requirement for multi- instrumental image processing (X-ray film to digital scanner) by integrating the image and sample analysis in one machine. This is expected to significantly improve experimental rigor and reproducibility by reducing sampling variables that can be inadvertently introduced during handling between multiple machines. Furthermore, this technology is much more environmentally friendly by avoiding the need for dangerous chemical disposal. We respectfully submit this request for Administrative Supplemental Funding to continue NINDS-funded research with new technology that will reduce our environmental footprint and increase rigor and reproducibility to conduct work vital to the completion of the proposed studies.

我们目前通过NINDS的R01 NS128574的壁外资金，标题为慢性间歇性缺氧和 高温启动，结合了多个取决于可视化和的蛋白质组学实验 跨体外和体内组织样品的蛋白质表达模式定量。此外， 在整个赠款提案中都使用了蛋白质印迹实验，以支持追踪的关键实验 周围神经元和巨噬细胞蛋白标记物表达谱。不幸的是，我们的主要部分 用于处理和可视化蛋白质印迹膜的机械已属于不可逆转的状态 失修，我们需要立即替换才能继续在应用程序中提出的实验。 我们目前容纳了18岁的最小服务X射线开发人员和处理器，以可视化Western 印迹膜。该开发人员需要每月服务才能用环保的清洁和替换水库 - 危险的开发人员和固定解决方案，以生产薄膜，然后进行数字扫描和量化 NIH图像1.62的频带光密度测定法确定蛋白质表达模式。几个月前，有2个 三个必要的储液泵故障。用服务估算更换破损泵的替换成本 技术人员的成本比全新的机器高，从而迫使我们停止在实验室中使用它 用于蛋白质组学研究。 从那以后，我们征集了投标，以确定如何用化学发光替代破裂的开发人员 成像师。我们收到了多家公司的几句报价，并为您提交附带的报价 考虑。 Thermofisher Life Technologies Ibright CL750数字成像系统是一种经济的 替换过时的最小X射线开发人员。这个成像系统允许成像 化学发光蛋白质印迹，蛋白质凝胶和DNA凝胶，并取代了多数的要求 通过将图像和样品分析整合在 一台机器。预计这将显着提高实验性的严格性和可重复性。 在多台机器之间处理过程中可以无意间引入的采样变量。 此外，通过避免需要危险化学物质，这项技术在环保方面更加友好 处理。 我们恭敬地提交此要求进行管理补充资金，以继续由Ninds资助的研究 采用新技术，可以减少我们的环境足迹，并增加执行的严格性和可重复性 对拟议研究的完成至关重要。