Development of novel synNotch CART cell therapy in adult patients with recurrent EGFRvIII+ glioblastoma

开发新型 synNotch CART 细胞疗法治疗复发性 EGFRvIII 胶质母细胞瘤成人患者

基本信息

批准号：
10689805
负责人：
Hideho Okada
金额：
$ 25.35万
依托单位：
UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-09-10 至 2026-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10689805
关键词：
Adolescent Adopted Adult Antigens B lymphoid malignancy Biodistribution Blood Brain Brain Neoplasms Cell Therapy Cells Central Nervous System Clinical Clinical Trials Collaborations Data Development Dose Engraftment Enrollment Epidermal Growth Factor Receptor Funding Glioblastoma Goals Good Manufacturing Process Grant Heterogeneity Homing Human Immunologic Monitoring Immunotherapy In Vitro Infiltration Inflammation Intravenous Intravenous infusion procedures Investigational Drugs Investigational New Drug Application Lentivirus Lentivirus Vector Malignant Neoplasms Maximum Tolerated Dose Medical Memory Methods Nervous System New Drug Approvals Operative Surgical Procedures Organ Patients Pharmacodynamics Phase Phase I Clinical Trials Phenotype Pre-Clinical Model Procedures Process Recurrence Research Personnel Resected Safety Signal Transduction Solid Surface Antigens System T cell therapy T-Lymphocyte Testing Tissues Toxic effect Toxicology Translation Process Tumor Tissue United States National Institutes of Health blood-brain tumor barrier body system cancer immunotherapy chimeric antigen receptor chimeric antigen receptor T cells cohort cytotoxicity design epidermal growth factor receptor VIII exhaustion experience first-in-human in vivo innovation invention manufacture manufacturing process mutant neoplastic cell notch protein novel patient derived xenograft model phase 1 study phase I trial preclinical efficacy prospective quality assurance receptor stem tumor ultrasound

项目摘要

Development of safe and effective (CAR)-transduced T cell (CART) therapy for glioblastoma (GBM) needs to overcome multiple challenges, including on-target off-tumor toxicity, heterogeneity of antigen expression, and exhaustion of CART cells. There are no GBM-specific surface antigens that are uniformly present in tumor tissue. Mutant epidermal growth factor receptor (EGFRvIII), for example, is GBM-specific but its expression is heterogenous within the tumor. On the other hand, while non-mutant GBM-associated antigens (GAAs), such as EphA2 and IL-13Rα2, are more uniformly expressed in GBM, their expression in other organs outside the central nervous system (CNS) raises concern for off-tumor toxicity. As a way to safely and effectively target GAAs, we have adopted a novel synthetic Notch “synNotch” receptor system and developed innovative T cells. In this system, the first antigen, which is expressed exclusively on brain or GBM cells (e.g. EGFRvIII), primes the T cells to induce expression of a CAR that recognizes IL-13Rα2 and EphA2, thereby eradicating GBM cells expressing either EphA2 or IL-13α2. Our data show that EGFRvIII-synNotch primed IL-13Rα2/EphA2 CAR are effectively but restrictedly activated by EGFRvIII as the GBM-specific signal, leading to complete eradication of patient-derived xenografts with heterogeneous EGFRvIII expression but without attacking IL-13Rα2/EphA2- positive cells outside of CNS. Furthermore, these synNotch-CAR T cells were significantly more efficacious than conventional, constitutively expressed IL-13Rα2/EphA2 CAR T cells, and were associated with excellent persistence (>100 days in vivo). Taken together, our data indicate that synNotch CAR T cells can revolutionize the CAR T therapy for solid cancers by overcoming the off-tumor toxicity, antigen heterogeneity and lack of persistence. Our goals are to establish the process for development of Good Manufacturing Practice products of EGFRvIII-primed IL-13Rα2/EphA2 CAR T cells, to obtain an Investigational New Drug (IND) approval, and to develop and conduct a phase I trial in patients with recurrent GBM. In Aim 1, we will generate GMP-grade lentiviral vector and establish standard procedures for GMP-grade manufacturing of the EGFRvIII-primed IL-13Rα2/ EphA2 CAR T cells. In Aim 2, we will complete both in vitro and in vivo studies that are required for submission of an IND. In vitro studies include confirmation of antigen-specific priming, specific cytotoxicity, and absence of replication competent lentivirus. In vivo, we will confirm preclinical efficacy, toxicology, tissue biodistribution, and engraftment as well as persistence of synNotch CART cells. In Aim 3, in a first-in-human phase 1 clinical trial, we will determine safety and toxicity of the synNotch CART cells in patients with recurrent EGFRvIII+ GBM. In a subsequent expansion cohort, in patients with recurrent EGFRvIII+ GBM, we will infuse synNotch CART cells intravenously prior to surgery, and then evaluate infiltration, priming and function of the infused cells in the resected GBM. These studies will allow us to determine optimal dosing based not only on tolerability but also on pharmacodynamic assessments.

开发安全有效的 (CAR) 转导 T 细胞 (CART) 治疗胶质母细胞瘤 (GBM) 需要克服多重挑战，包括靶向肿瘤外毒性、抗原表达的异质性，以及 CART 细胞耗尽肿瘤中不存在均匀存在的 GBM 特异性表面抗原。例如，突变表皮生长因子受体 (EGFRvIII) 是 GBM 特异性的，但其表达是另一方面，非突变 GBM 相关抗原 (GAA) 则具有异质性。 EphA2 和 IL-13Rα2 在 GBM 中表达更为一致，它们在 GBM 以外的其他器官中表达作为一种安全有效的靶向方法，中枢神经系统（CNS）引发了对肿瘤外毒性的关注。 GAAs，我们采用了新型合成Notch“synNotch”受体系统并开发了创新的T细胞。在该系统中，仅在脑或 GBM 细胞上表达的第一个抗原（例如 EGFRvIII）引发 T细胞诱导识别IL-13Rα2和EphA2的CAR表达，从而根除GBM细胞表达 EphA2 或 IL-13α2 我们的数据显示 EGFRvIII-synNotch 引发的 IL-13Rα2/EphA2 CAR 是 EGFRvIII 作为 GBM 特异性信号受到限制但有效激活，从而彻底根除患者来源的异种移植物具有异质 EGFRvIII 表达，但不攻击 IL-13Rα2/EphA2- 此外，这些 synNotch-CAR T 细胞显着更有效。与传统的、组成型表达的 IL-13Rα2/EphA2 CAR T 细胞相比，并且与优异的持久性（> 体内 100 天），我们的数据表明 synNotch CAR T 细胞可以带来革命性的变化。 CAR T疗法通过克服肿瘤外毒性、抗原异质性和缺乏我们的目标是建立良好生产规范的发展流程。 EGFRvIII 引发的 IL-13Rα2/EphA2 CAR T 细胞的产品，以获得研究性新药 (IND) 批准，并在复发性 GBM 患者中开发和进行 I 期试验，我们将在目标 1 中进行。生成 GMP 级慢病毒载体并建立 GMP 级生产的标准程序 EGFRvIII 引发的 IL-13Rα2/ EphA2 CAR T 细胞在目标 2 中，我们将完成体外和体内研究：提交 IND 需要进行体外研究，包括确认抗原特异性引发、特异性。细胞毒性，以及缺乏复制能力的慢病毒，我们将在体内确认临床前功效，在目标 3 中，研究了 synNotch CART 细胞的毒理学、组织生物分布和植入以及持久性。这是一项首次人体 1 期临床试验，我们将确定 synNotch CART 细胞在在随后的扩展队列中，复发性 EGFRvIII+ GBM 患者。 GBM，我们会在术前静脉注射synNotch CART细胞，然后评估浸润、启动以及切除的 GBM 中输注细胞的功能这些研究将使我们能够确定最佳剂量。不仅基于耐受性，还基于药效学评估。