Molecular Profiling (MP) Core G

分子分析 (MP) 核心 G

• 批准号: 10689317
• 负责人: Matt Huentelman
• 金额: $ 293.22万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10689317
• 关键词: Age-associated memory impairment; Aging; Alzheimer' s Disease; Alzheimer' s disease related dementia; Amyloid; Area; B cell repertoire; B-Cell Antigen Receptor; Bar Codes; Biological Assay; Biological Markers; Blood; Brain; Cells; Cognitive; Cognitive aging; Communities; Consultations; Country; DNA; Data; Data Analyses; Data Science Core; Data Set; Deposition; Development; Diabetes Mellitus; Dryness; Ensure; Epigenetic Process; Genotype; Goals; Hypertension; Immune; Immunologic Markers; Immunologist; Individual; Inflammatory; Letters; Longevity; Measurement; Measures; Mediating; Molecular; Molecular Analysis; Molecular Profiling; Neurodegenerative Disorders; Participant; Pathway interactions; Peptides; Plasma; Play; Process; RNA; Research; Research Personnel; Resources; Risk Factors; Role; Sampling; Scientist; Serum; Signal Pathway; Sleep; Specimen; Spottings; T-Lymphocyte; Testing; Universities; Viral; Whole Blood; Work; biobank; biomarker development; biomarker discovery; blood fractionation; cognitive performance; cohort; data repository; data submission; data warehouse; database of Genotypes and Phenotypes; exosome; experience; genetic risk factor; genome sequencing; genome-wide; genomic data; healthspan; human subject; interest; member; memory consolidation; next generation sequencing; novel; polygenic risk score; protein biomarkers; public database; repository; success; tau Proteins; transcriptome sequencing; whole genome

SUMMARY/ABSTRACT: Molecular Profiling Core G The goals of the Molecular Profiling Core (MP) Core G are to (a) perform the duties of the study-wide biobank including tracking and distribution of samples to Project 3 and other Cores and Projects as needed, (b) facilitate the rapid public sharing of biospecimens through NCRAD (National Centralized Repository for Alzheimer’s Disease and Related Dementias) by providing them with twice yearly sample shipments for deposition and administration to the general scientific community, (c) generate molecular data for biospecimens collected from Projects 1 and 2, (d) provide iterative re-analysis of genomic data (RNA and DNA) as necessitated by the activities of Project 4 to ensure that all study data is up-to-date with regards to their respective analytical and annotation pipelines, and (e) provide expert consultation on all aspects of biomarker discovery and development to the Precision Aging Network (PAN) investigators. Project 1 biospecimens will consist of 10,000 dried blood spot cards (DBS). These DBS samples are self- collected from the nationally distributed cohort of individuals who participate in the MindCrowd-Expanded (MC-Expanded) cohort. The MP Core will perform APOE and array-based genotyping on these specimens and deposit the raw and processed data into the study data repository via the Data Science (DS) Core. These data will be utilized by Project 1 investigators to identify genetic risk factors that are associated with, or that modify, cognitive performance on the MC-Expanded test battery. Additionally, through the use of the genome- wide array data, each participant will be assigned individualized polygenic risk score (PRS) pertaining to the various risk factors detailed in Project 2 (e.g., Alzheimer’s disease, hypertension, diabetes, sleep, etc.). Project 2 biospecimens will consist of 1,620 plasma, serum, and CSF samples collected from the face-to-face characterized cohort. Several molecular profiling datasets will be generated from these samples including; whole genome sequencing, CSF biomarkers, immune and inflammatory measurements, pan-viral exposures, T- and B-cell receptor repertoires, whole blood and cell-free exosome RNA sequencing, and Horvath epigenetic clock determinations among others (detailed further in the MP Core). These data will be quality controlled and uploaded to the study data repository in both raw and processed formats for use by investigators in the Projects and Cores. The acquired high-quality and wide-ranging molecular data will play significant roles in answering major research questions in our proposed projects, and can also be used by the national research community studying cognitive aging, Alzheimer’s disease (AD), and related dementias (ADRD).

摘要/摘要：分子分析核心 G 分子分析核心 (MP) 核心 G 的目标是 (a) 履行研究范围内的职责 生物样本库，包括根据需要跟踪样本并将其分发到项目 3 和其他核心和项目， (b) 通过 NCRAD（国家生物样本中央存储库）促进生物样本的快速公开共享 阿尔茨海默病和相关痴呆症），每年为他们提供两次样本运送 向一般科学界沉积和管理，(c) 生成分子数据 从项目 1 和 2 收集的生物样本，(d) 提供基因组数据（RNA 和 DNA）作为项目 4 活动所必需的，以确保所有研究数据都是最新的 他们各自的分析和注释管道，以及（e）提供有关各方面的专家咨询 为精准衰老网络 (PAN) 研究人员提供生物标志物发现和开发。 项目 1 生物样本将包含 10,000 张干血斑卡 (DBS)，这些 DBS 样本是自检的。 从参加 MindCrowd-Expanded 的全国分布的个人队列中收集 (MC-Expanded) 队列将对这些样本进行 APOE 和基于芯片的基因分型。 通过数据科学 (DS) 核心将原始数据和处理后的数据存入研究数据存储库。 项目 1 的研究人员将利用数据来识别与之相关的遗传风险因素，或者 修改 MC-Expanded 测试电池的认知性能。 广泛的数据，每个参与者将被分配与相关的个性化多基因风险评分（PRS） 项目 2 中详述的各种危险因素（例如阿尔茨海默病、高血压、糖尿病、睡眠等）。 项目 2 生物样本将包括从面对面采集的 1,620 份血浆、血清和脑脊液样本 将从这些样本中生成几个分子分析数据集，包括： 全基因组测序、脑脊液生物标志物、免疫和炎症测量、泛病毒暴露、 T 细胞和 B 细胞受体库、全血和无细胞外泌体 RNA 测序以及 Horvath 表观遗传时钟测定等（在 MP 核心中进一步详细说明）这些数据将是高质量的。 以原始和处理后的格式进行控制并上传到研究数据存储库，供研究人员使用 项目和核心的调查员。 所获得的高质量、广泛的分子数据将在回答重大问题方面发挥重要作用。 我们提出的项目中的研究问题，也可以被国家研究界使用 研究认知衰老、阿尔茨海默病 (AD) 和相关痴呆症 (ADRD)。