Molecular Profiling (MP) Core G

分子分析 (MP) 核心 G

• 批准号: 10689317
• 负责人: Matt Huentelman
• 金额: $ 293.22万
• 依托单位: UNIVERSITY OF ARIZONA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10689317
• 关键词: Age-associated memory impairment; Aging; Alzheimer' s Disease; Alzheimer' s disease related dementia; Amyloid; Area; B cell repertoire; B-Cell Antigen Receptor; Bar Codes; Biological Assay; Biological Markers; Blood; Brain; Cells; Cognitive; Cognitive aging; Communities; Consultations; Country; DNA; Data; Data Analyses; Data Science Core; Data Set; Deposition; Development; Diabetes Mellitus; Dryness; Ensure; Epigenetic Process; Genotype; Goals; Hypertension; Immune; Immunologic Markers; Immunologist; Individual; Inflammatory; Letters; Longevity; Measurement; Measures; Mediating; Molecular; Molecular Analysis; Molecular Profiling; Neurodegenerative Disorders; Participant; Pathway interactions; Peptides; Plasma; Play; Process; RNA; Research; Research Personnel; Resources; Risk Factors; Role; Sampling; Scientist; Serum; Signal Pathway; Sleep; Specimen; Spottings; T-Lymphocyte; Testing; Universities; Viral; Whole Blood; Work; biobank; biomarker development; biomarker discovery; blood fractionation; cognitive performance; cohort; data repository; data submission; data warehouse; database of Genotypes and Phenotypes; exosome; experience; genetic risk factor; genome sequencing; genome-wide; genomic data; healthspan; human subject; interest; member; memory consolidation; next generation sequencing; novel; polygenic risk score; protein biomarkers; public database; repository; success; tau Proteins; transcriptome sequencing; whole genome

SUMMARY/ABSTRACT: Molecular Profiling Core G The goals of the Molecular Profiling Core (MP) Core G are to (a) perform the duties of the study-wide biobank including tracking and distribution of samples to Project 3 and other Cores and Projects as needed, (b) facilitate the rapid public sharing of biospecimens through NCRAD (National Centralized Repository for Alzheimer’s Disease and Related Dementias) by providing them with twice yearly sample shipments for deposition and administration to the general scientific community, (c) generate molecular data for biospecimens collected from Projects 1 and 2, (d) provide iterative re-analysis of genomic data (RNA and DNA) as necessitated by the activities of Project 4 to ensure that all study data is up-to-date with regards to their respective analytical and annotation pipelines, and (e) provide expert consultation on all aspects of biomarker discovery and development to the Precision Aging Network (PAN) investigators. Project 1 biospecimens will consist of 10,000 dried blood spot cards (DBS). These DBS samples are self- collected from the nationally distributed cohort of individuals who participate in the MindCrowd-Expanded (MC-Expanded) cohort. The MP Core will perform APOE and array-based genotyping on these specimens and deposit the raw and processed data into the study data repository via the Data Science (DS) Core. These data will be utilized by Project 1 investigators to identify genetic risk factors that are associated with, or that modify, cognitive performance on the MC-Expanded test battery. Additionally, through the use of the genome- wide array data, each participant will be assigned individualized polygenic risk score (PRS) pertaining to the various risk factors detailed in Project 2 (e.g., Alzheimer’s disease, hypertension, diabetes, sleep, etc.). Project 2 biospecimens will consist of 1,620 plasma, serum, and CSF samples collected from the face-to-face characterized cohort. Several molecular profiling datasets will be generated from these samples including; whole genome sequencing, CSF biomarkers, immune and inflammatory measurements, pan-viral exposures, T- and B-cell receptor repertoires, whole blood and cell-free exosome RNA sequencing, and Horvath epigenetic clock determinations among others (detailed further in the MP Core). These data will be quality controlled and uploaded to the study data repository in both raw and processed formats for use by investigators in the Projects and Cores. The acquired high-quality and wide-ranging molecular data will play significant roles in answering major research questions in our proposed projects, and can also be used by the national research community studying cognitive aging, Alzheimer’s disease (AD), and related dementias (ADRD).

摘要/摘要：分子分析核心G 分子分析核心（MP）核心G的目标是（a）执行整个学习的职责 生物库，包括跟踪和分发样品3项目3以及其他需要的其他核心和项目， （b）通过NCRAD（国家集中式存储库 阿尔茨海默氏病和相关痴呆症）通过为它们提供两次年度样本出货量 对一般科学界的沉积和管理，（c）生成分子数据 从项目1和2，（d）收集的生物测量提供了基因组数据的迭代重新分析（RNA和 DNA）根据项目4的活动必要 他们各自的分析和注释管道，以及（e）就所有方面的各个方面提供专家咨询 生物标志物发现和开发到精确的老化网络（PAN）研究人员。 项目1 Biospecimens将由10,000张干血点卡（DBS）组成。这些DBS样本是自我的 从参与Mindcrowd扩展的个人的全国分布人群中收集 （MC扩展）队列。 MP核心将在这些标本上执行基于APOE和数组的基因分型，并且 通过数据科学（DS）核心将原始数据和处理的数据存储到研究数据存储库中。这些 项目1调查人员将利用数据来确定与之相关的遗传危险因素或 修改MC扩展的测试电池上的认知性能。另外，通过使用基因组 广泛的数组数据，每个参与将被分配给与之有关的个性化多基因风险评分（PRS） 项目2中详细介绍的各种风险因素（例如，阿尔茨海默氏病，高血压，糖尿病，睡眠等）。 项目2 Bioscimens将由面对面收集的1,620个血浆，血清和CSF样品组成 特征是队列。这些样品将生成几个分子分析数据集，包括： 整个基因组测序，CSF生物标志物，免疫和炎症测量，泛病毒暴露， T-和B细胞受体库，全血和无细胞外泌体RNA测序以及Horvath 表观遗传时钟的测定等（进一步详细介绍了MP核心）。这些数据将是质量 以原始和处理的格式进行控制并上传到研究数据存储库，以供您使用 项目和核心的调查人员。 获得的高质量和宽范围的分子数据将在回答主要方面发挥重要作用 我们提出的项目中的研究问题，也可以由国家研究界使用 研究认知衰老，阿尔茨海默氏病（AD）和相关痴呆症（ADRD）。