TRP channel regulation of feeding behavior in Drosophila

TRP 通道对果蝇摄食行为的调节

• 批准号: 10683242
• 负责人: WILLIAM W JA
• 金额: $ 48.3万
• 依托单位: UNIVERSITY OF FLORIDA
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-24 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10683242
• 关键词: Acute; Adult; Affect; Agriculture; Animal Behavior; Animals; Behavior; Calcium; Circadian Rhythms; Code; Consumption; Culicidae; Diet; Disease; Disease Vectors; Dissection; Drosophila genus; Drosophila melanogaster; Eating; Energy Intake; Event; Family; Fasting; Feeding behaviors; Food; Food Processing; Gene Expression; Genes; Genetic; Habits; Health; Homeostasis; Human; Hunger; Image; Ingestion; Insecta; Intake; Intermittent fasting; Intervention; Invertebrates; Knowledge; Life; Link; Logic; Mammals; Measures; Mechanical Stimulation; Mediating; Memory; Metabolic; Metabolism; Methods; Modeling; Molecular; Monitor; Neurologic; Neurons; Neuropeptides; Neurotransmitters; Nutrient; Nutritional; Organism; Pain; Painless; Pattern; Peptides; Pesticides; Phenotype; Physiological; Physiology; Property; Proteins; Publishing; Regulation; Role; Satiation; Sensory; Sensory Receptors; Series; Signal Transduction; Sleep; Stomach; Stretching; System; TRP channel; Testing; Time; age related; design; dietary; feeding; fly; food consumption; hedonic; improved; insight; neural; neurogenetics; neuromechanism; nutrition; optogenetics; pharmacologic; prevent; receptor; response; shear stress; temporal measurement; tool

PROJECT SUMMARY: TRP channel regulation of feeding behavior in Drosophila Feeding is one of the most fundamental of all animal behaviors. In mammals, diverse signals, including chemosensory responses to food properties and mechanical stimulation of the gut, regulate satiation and feeding behavior. These mechanisms, or analogous systems, are well-conserved in invertebrates. Although many neuropeptides and neurotransmitters have been identified that signal satiation or satiety, much less is known about how gut stretch and other mechanosensory forces are signaled to regulate feeding behavior. Due to the remarkable conservation in their basic physiological and neurological properties, studies in Drosophila have revolutionized our broad understanding of animal behavior. Despite the successful use of flies in investigating paradigms such as sleep, circadian rhythm, and memory, only recently have tools for measuring fly food intake facilitated the study of feeding behavior. This project will use Drosophila melanogaster to investigate the regulation of meal intake. New methods allow undisturbed real-time measurements of food consumption in freely behaving adult flies that resolve with unrivalled accuracy the effects of diet and hunger on meal size. An experimental framework is established for defining fly meals and to show their basic regulation by manipulation of the diet, internal hunger state, and circadian rhythms. Using the powerful genetic tools available in Drosophila, preliminary results reveal the involvement of transient receptor potential (TRP) channels in the regulation of meal intake. TRP channels define a large family of sensory receptors, and much is still unknown about how they function as chemo-, mechano-, and other types of receptors. The proposed studies will dissect the role of TRP channel signaling in satiation and meal size control, taking advantage of the tools available in the simplified Drosophila model. Given the increasing importance of meal intake and patterning on physiology and health—regardless of total caloric intake—the proposed studies have the potential to reveal key insights on the role of TRP channel signaling on regulating prandial behavior, physiology, and metabolism. The proposed studies may also eventually inform the design of pesticides targeting TRP channels or feeding behavior in other insects, including agricultural pests and disease vectors such as mosquitos.

项目摘要：果蝇中喂养行为的TRP通道调节 喂养是所有动物行为中最基本的一种。在哺乳动物中，潜水员信号，包括 对食物特性的化学感应反应以及肠道的机械模拟，调节饱腹感和 喂养行为。这些机制或类似的系统在无脊椎动物中保存良好。虽然 已经确定了许多神经肽和神经递质的信号满意度或满意度，更不用说 知道如何签署肠道拉伸和其他机械学力来调节进食行为。 由于其基本的生理和神经系统特性的显着保护，研究 果蝇彻底改变了我们对动物行为的广泛理解。尽管成功使用了 苍蝇调查诸如睡眠，昼夜节律和记忆之类的范式，直到最近才有工具 测量食物摄入量准备了喂养行为的研究。这个项目将使用果蝇 Melanogaster调查进餐摄入的调节。新方法允许实时不受干扰 以无与伦比的准确性解决的自由行为成年苍蝇中食物消耗的测量 饮食和饥饿对餐大小的影响。建立了一个实验框架，用于定义饮食和 通过操纵饮食，内部饥饿状态和昼夜节律来显示他们的基本调节。使用 果蝇可用的强大遗传工具，初步结果揭示了瞬态接收器的参与 饮食摄入调节中的电势（TRP）通道。 TRP频道定义了一个大家族的感觉 接收器，并且对于它们如何充当化学，机械和其他类型的方式仍然不知道 接收者。拟议的研究将阐明TRP信号信号在饱腹和进餐大小中的作用 控制，利用简化的果蝇模型中可用的工具。考虑到增加 进餐摄入量和对生理学和健康的构图的重要性 - 无热量摄入量 - 提出的研究有可能揭示有关TRP信号信号在调节的作用的关键见解 实践行为，生理和代谢。拟议的研究有时也可能告知设计 针对TRP通道的农药或其他昆虫的饲料行为，包括农业害虫和 蚊子等疾病载体。