Vector Immunology Core

载体免疫学核心

基本信息

批准号：
10674936
负责人：
Guangping Gao
金额：
$ 33.5万
依托单位：
UNIV OF MASSACHUSETTS MED SCH WORCESTER
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-09 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10674936
关键词：
Antibodies Antigens B-Lymphocytes Binding Biological Assay Capsid Cell Therapy Clinic Clinical Trials Clustered Regularly Interspaced Short Palindromic Repeats Cystic Fibrosis Data Disease Disease model Dose Engineering Enzyme-Linked Immunosorbent Assay Evaluation Ferrets Flow Cytometry Gene Delivery Gene Transduction Agent Goals Grant Human Humoral Immunities Immune Immune response Immune system Immunity Immunological Models Immunology Immunology procedure Inflammatory Measures Mediating Modeling Monkeys Mus Neutralizing antibody assay Outcome Patients Program Research Project Grants Proteins Protocols documentation Reagent Regulatory T-Lymphocyte Resources Respiratory Tract Infections Role Serious Adverse Event Serology Stains Standardization Steroids T cell response T-Lymphocyte Testing Therapeutic Transgenes Virus adeno-associated viral vector alpha 1-Antitrypsin Deficiency cell mediated immune response clinically relevant cytokine cytotoxic disease phenotype enzyme linked immunospot assay gene product gene therapy immunogenic mouse model novel response therapeutic gene transgene expression vector

项目摘要

CORE A - SUMMARY/ABSTRACT: The Vector Immunology Core will directly support all four projects of this program project grant by providing reliable, consistent and standardized immunological assays. It will focus on creating, validating and performing immunological assays to characterize B-cell and T-cell immune responses to AAV, in mice and ferrets. It will also assay booth immune responses to capsid and to transgene product. The core will support all four projects by characterizing B-cell responses by transduction inhibition based NAB assays and ELISAs for capsid binding antibodies and anti-transgene antibodies. It will develop and standardize assays for evaluating ferret B-cell responses. It will additionally characterize T-cell responses by ELISPOT, and cytokine profiling through both ELISA and flow-based cytokine bead assays. Finally, it will develop flow cytometry staining protocols for the evaluation of Ferret immune responses, which are currently not well characterized. Overall the immunology core will provide valuable data, support and resources to all four projects within this program project grant, which will allow for better understanding of the role the immune system in gene and cell therapies.

核心 A - 摘要/摘要：载体免疫学核心将直接支持该计划项目的所有四个项目通过提供可靠、一致和标准化的免疫学检测来授予资助。它将重点关注创建、验证和执行免疫学测定来表征 B 细胞和 T 细胞小鼠和雪貂对 AAV 的免疫反应。它还将检测展位的免疫反应衣壳和转基因产物。该核心将通过表征 B 细胞来支持所有四个项目基于 NAB 测定和衣壳结合 ELISA 的转导抑制反应抗体和抗转基因抗体。它将开发和标准化评估方法雪貂 B 细胞反应。它还将通过 ELISPOT 表征 T 细胞反应，以及通过 ELISA 和基于流的细胞因子珠分析进行细胞因子分析。最后，它将开发用于评估雪貂免疫反应的流式细胞术染色方案，其中目前尚未得到很好的表征。总体而言，免疫学核心将提供有价值的数据，为本计划项目拨款内的所有四个项目提供支持和资源，这将允许更好地了解免疫系统在基因和细胞疗法中的作用。