JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP)

JHU-Mayo-NIA 小鼠衰老图谱计划 (JMN-MSMP)

基本信息

批准号：
10673112
负责人：
Darren Baker
金额：
$ 131.64万
依托单位：
JOHNS HOPKINS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10673112
关键词：
3-Dimensional Adipose tissue Age Aging Atlases Biological Biological Markers Bone Marrow Brain Cataloging Cell Aging Cell Communication Cells Code Collaborations Communication Computer Analysis Data Data Analyses Data Coordinating Center Data Science Data Set Decision Making Deposition Development Ensure Epigenetic Process FAIR principles Funding Genomics Goals Heterogeneity Hippocampus Human Image Immunology Infrastructure Kidney Laboratory mice Lead Leadership Liver Longevity Lymphoid Tissue Maps Methodology Methods Molecular Monitor Mouse Strains Mus National Institute on Aging Outcome Pathology Phenotype Physiological Physiology Plasma Policies Prevalence Process Productivity Proteomics Protocols documentation Reporter Reproducibility Research Research Personnel Resource Sharing Resources Sampling Serum Shipping Skeletal Muscle Skin Specimen Standardization Techniques Technology Tissue Banks Tissues age related animal data circulating biomarkers computing resources data integration data sharing data submission dimensional analysis imaging modality immunohistochemical markers in vivo innovation learning algorithm novel novel marker programs repository scale up screening senescence sex single cell analysis single cell sequencing single cell technology single-cell RNA sequencing timeline tissue archive tissue mapping transcriptomics transfer learning

项目摘要

The overall goal of the JHU-Mayo-NIA Murine Senescence Mapping Program (JMN-MSMP) is to create an Atlas of Senescence in Murine Tissues across the lifespan. The proposal encompasses unique and novel biological and computational resources and strategies to map senescent cells (SnCs) in murine tissues. Biological resources include aging mouse colonies of multiple strains, including those with reporters for identification of SnCs. We also have access to a robust murine tissue bank and cataloging infrastructure for aging tissues, including those with age-related pathology. Computational resources include single-cell sequencing and spatial transcriptomics expertise, transfer learning algorithms that can identify SnCs and elucidate cell communication from single-cell datasets, and integration of genomics and multiple imaging modalities to identify SnCs in tissues in 2D and 3D. For this proposal, the JMN-MSMP team will focus first on developing a timeline of the emergence of senescence markers in plasma/serum in tissues from the National Institute of Aging murine aging program including brain. Importantly, we have archived tissues from two genetically distinct strains of laboratory mice of both sexes over their lifespan that will allow us to implement a rigorous and multidimensional analysis as soon as the Program is funded. This will be followed by in depth single cell analysis of selected tissues for senescence mapping. The JMN-MSMP will consist of three Core components: the Administrative Core (AC), Biological Analysis Core (BAC), and Data Analysis Core (DAC). The Specific Aims of the JMN-MSMP are: Aim 1. Overall JMN-MSMP Research Strategy: Integrate genomics and imaging strategies to build a comprehensive Atlas of Senescence in Murine Tissues, and to define the physiological impact of senescent cells. Aim 2. Overall JMN-MSMP Management Strategy, Goals, and Milestones: Provide infrastructure to support an integrated research effort toward creation of the Atlas. Aim 3. JMN-MSMP Resource Sharing: Ensure that tissues, data, methods, and technologies are available to other TMCs, and that appropriate data deposition and sharing policies are in place.

Jhu-mayo-nia鼠衰老映射计划（JMN-MSMP）的总体目标是创建一个地图集整个寿命的鼠组织衰老。该提案包括独特而新颖的生物学以及在鼠组织中绘制衰老细胞（SNC）的计算资源和策略。生物资源包括衰老的鼠标菌落多种菌株，包括那些识别记者的菌株 SNC。我们还可以使用坚固的鼠组织库以及用于衰老组织的基础设施分类，包括患有年龄相关病理的人。计算资源包括单细胞测序和空间转录组学专业知识，可以识别SNC并阐明细胞通信的转移学习算法从单细胞数据集以及基因组学和多种成像方式的整合来识别组织中的SNC 在2D和3D中。对于此建议，JMN-MSMP团队将首先专注于开发出现的时间表美国国家老化鼠老化计划的组织中血浆/血清中衰老标记包括大脑。重要的是，我们已经从两种遗传上不同的实验室菌株的组织存档两性在他们的寿命中都可以使我们能够尽快实施严格而多维的分析由于该计划的资助。接下来是深度的衰老组织的单细胞分析映射。 JMN-MSMP将由三个核心组成部分组成：行政核心（AC），生物学分析核心（BAC）和数据分析核心（DAC）。 JMN-MSMP的具体目的是：目的1。总体JMN-MSMP研究策略：整合基因组学和成像策略以建立一个鼠组织中衰老的综合地图集，并定义衰老的生理影响细胞。目标2。总体JMN-MSMP管理策略，目标和里程碑：提供基础架构以支持为创建地图集的综合研究工作。目标3。JMN-MSMP资源共享：确保可以使用组织，数据，方法和技术其他TMC以及适当的数据沉积和共享策略也存在。