A turn-key optogenetics and electrophysiology measurement system

交钥匙光遗传学和电生理学测量系统

• 批准号: 8979335
• 负责人: DAVID A JOHNSON
• 金额: $ 46.75万
• 依托单位: PINNACLE TECHNOLOGY, INC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-07-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8979335
• 关键词: Aging; Animals; Area; Astrocytes; Behavior; Behavioral; Biosensor; Brain; Brain region; Cannulas; Cell Culture Techniques; Cells; Chemistry; Computer software; Data; Development; Development Plans; Devices; Disease; Electroencephalography; Electromyography; Electronics; Electrophysiology (science); Elements; Equipment; Event; Feedback; Fiber; Fiber Optics; Genetic; Goals; Head; Heart Diseases; Hospitalization; Individual; Joints; Kansas; Knowledge; Laboratories; Lasers; Light; Longevity; Malignant Neoplasms; Measurement; Mechanics; Medical center; Mental disorders; Methods; Mus; Neuraxis; Neurons; Neurosciences; Neurotransmitters; New York; Operative Surgical Procedures; Opsin; Optical Methods; Optics; Oranges; Output; Pharmacologic Substance; Phase; Physiology; Premature Mortality; Process; Progress Reports; Protocols documentation; Rattus; Research; Research Personnel; Rodent; Signal Transduction; Single Seizures; Solutions; Source; Stimulus; System; Systems Analysis; Techniques; Time; United States; Universities; Validation; Visual; Wireless Technology; Work; base; behavior measurement; burden of illness; commercialization; cost; design; digital; experience; improved; in vivo; multidisciplinary; optogenetics; product development; prototype; public health relevance; research study; severe mental illness; skills; software systems; success

 DESCRIPTION (provided by applicant): The goal of this project is to design and commercialize tethered and wireless turn-key optogenetics and electrophysiological/neurotransmitter/behavior measurement systems for use in mice and rats. Optogenetics harnesses a combination of genetic and optical methods to directly control neuronal events in specific cells of the central nervous system. These methods are broadly applicable, but can be specifically used to provide an unprecedented understanding of cortical activity and aging. The optogenetics field is maturing and there are numerous commercial sources for optogenetic components; however, the technique requires a multidisciplinary skill set including chemistry, optics, physiology, electronics, mechanics, software, and systems analysis. To date, any single experiment requires a system designed from individual, component parts. Many researchers also have existing equipment that they desire to incorporate into a full optogenetics system. This may include lasers, cameras and potentially behavioral hardware and software platforms. In these situations, a digital timing protocol (TTL) is often used to maintain synchronization, but there are subtleties (device latency, etc.) to this approach that are often overlooked. The proposed system will be capable of delivering multiple, selectable wavelengths of light to one or more specific brain regions while simultaneously recording electrical signals, neurotransmitters and behavior in rodents throughout the lifespan of the animal. All synchronization between the electrophysiological, mechanical and visual inputs, and optical and stimulus outputs will be precisely controlled via a master timing, digital input/output platform as well as sophisticated software timing techniques. The LED fiber probe connects via a simple electrical connection to a headstage. This removes the need for fiber optic rotary joints and enables precise control of the amount of light delivered. When completed, this system will significantly improve scientific knowledge by providing a turn-key solution for researchers from multiple fields to seamlessly integrate optogenetic control alongside traditional pharmaceutical, aging and other studies.

 描述（由申请人提供）：该项目的目标是设计并商业化用于小鼠和大鼠的系留和无线统包光遗传学和电生理/神经递质/行为测量系统。光遗传学利用遗传和光学方法的组合来直接进行测量。这些方法广泛适用，但可以专门用于提供对皮层活动和衰老的前所未有的理解。光遗传学组件正在成熟，并且有许多商业来源；然而，该技术需要多学科技能，包括化学、光学、生理学、电子、机械、软件和系统分析，迄今为止，任何单一实验都需要单独设计的系统。许多研究人员还拥有他们希望整合到完整光遗传学系统中的现有设备，这可能包括激光器、相机和潜在的行为硬件和软件平台。在这些情况下，通常使用数字定时协议（TTL）。保持同步，但是这种方法有一些经常被忽视的微妙之处（设备延迟等），所提出的系统将能够向一个或多个特定的大脑区域传递多个可选择波长的光，同时记录啮齿类动物的电信号、神经递质和行为。在动物的整个生命周期中，电生理、机械和视觉输入以及光学和刺激输出之间的所有同步都将通过主定时、数字输入/输出平台以及先进的软件定时技术进行精确控制。通过与探头的简单电气连接，无需光纤旋转接头，并能够精确控制传输的光量。完成后，该系统将为多个研究人员提供交钥匙解决方案，从而显着提高科学知识。领域将光遗传学控制与传统制药、衰老和其他研究无缝集成。