Ocular Phenotyping Core

眼表型核心

基本信息

批准号：
10476371
负责人：
Timothy W Kraft
金额：
$ 13.1万
依托单位：
UNIVERSITY OF ALABAMA AT BIRMINGHAM
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-08-01 至 2026-07-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10476371
关键词：
Anatomy Angiography Animal Model Animals Anterior Area Award Basic Science Benchmarking Biological Assay Cells Clinical Collaborations Color Communities Contrast Sensitivity Core Facility Core Grant Cornea Custom Disease Dyes Electroretinography Engineering Environment Equipment Expenditure Experimental Designs Eye Funding Fundus photography Growth Health Heart Human Human Resources Image Individual Iris Laboratories Lead Microscope Microscopy Monitor Mus Ocular Physiology Operative Surgical Procedures Optic Disk Optical Coherence Tomography Optokinetic nystagmus Outcome Outcome Measure POU2F2 gene Participant Phenotype Physiological Postdoctoral Fellow Productivity Publications Rattus Research Personnel Research Support Retina Rodent S10 grant Scientist Signal Transduction Source Standardization Structure Students Surveys System Technology Testing Time Tissues Training Translational Research Tupaiidae Validation Vision Vision research Visual Visual Acuity Visualization Zebrafish adaptive optics scanning laser ophthalmoscopy animal resource animal tissue base behavior test body position body system design digital experience fluorescence imaging fundus imaging human subject imaging system in vivo instrument instrumentation interest lens mouse model multimodality novel diagnostics operation power analysis ranpirnase research facility response retinal imaging satisfaction statistics success tool vision science visual motor

项目摘要

UAB Center Core for Vision Research - Ocular Phenotyping Core Project/Summary Abstract Assessment of ocular structures and visual function is essential to both basic and translational research in vision science whether in animal models or human subjects. Widely used technologies include optical coherence tomography (OCT) for display of layered tissues in posterior and anterior segments, electroretinography (ERG, for massed retinal signals separable into multiple identified components), slit lamp for bio-microscopy of anterior and posterior segment, imaging of the fundus via multiple modes of visualization (color, autofluorescence, dye- based angiography, infrared reflectance), and optokinetic nystagmus (to assess visuomotor control, visual acuity and contrast sensitivity). In response to growing UAB vision researcher needs, the “Ocular Phenotyping Core” was established to encompass a comprehensive suite of instrumentation and to provide the necessary support for accurate ocular phenotyping. Specific instruments include Bioptigen 840 nm SD-OCT and Micron IV digital fundus camera for small animals, Optomotry optokinetic nystagmus in small animals including rats, mice and zebrafish, and Spectralis SDOCT for large animals and human donor eyes. An S10 grant awarded to Dr. Paul Gamlin in 2020 has allowed the purchase of three new tools that will greatly enhance ocular phenotyping of both large and small animal tissues 1) Zeiss Lumera 700 ReScan with Resight 700 operating microscope with intraoperative optical coherence tomography (OCT) imaging; 2) FLEX Module Spectralis OCT2 System for imaging the retina and optic nerve head with structural and angiographic OCT, in animals at various body positions; 3) Anterior Segment CASIA SS-1000 swept source OCT (Tomey Corp.) for imaging the cornea, iris, and lens in large animals, as well as the whole eye in small animals. This core will support 15 UAB Vision Scientists, including 13 with planned moderate to extensive all of whom are currently NEI R01-funded. The Director and Associate Director of this proposed core have extensive publication experience in electroretinography and OCT validation/ interpretation, respectively. New directions for the core will include the completion of a new LED-based ERG system that will simplify this testing. Further, an existing AOSLO system originally designed for human retinal imaging will be re-engineered in order to image rodent and tree shrew eyes in vivo. At the same time, capabilities for fluorescence imaging and cell-targeted photo-stimulation will be added. Additionally, Ocular Phenotyping Opportunities will be advertised to identify new ocular mouse models through full ocular phenotyping screens of mouse models generated by non-ocular scientists whose animals were originally generated to answer questions pertinent to their organ systems of interest.

UAB视觉研究中心核心 - 眼表型核心项目/摘要摘要眼睛结构和视觉功能的评估对于视觉中的基本和翻译研究至关重要科学在动物模型或人类受试者中。广泛使用的技术包括光学连贯性层析成像（OCT）用于显示后部和前部层的分层组织，电子模拟（ERG，对于大规模的视网膜信号分成多个已识别的组件）和后段，通过多种可视化模式（颜色，自动荧光，染料，染料）成像基于血管造影，红外反射率）和光（评估视觉运动，视力控制，视力和对比灵敏度）。为了响应不断增长的UAB视觉研究人员的需求，“眼表型核心” 成立以涵盖一套综合仪器，并提供必要的支持用于准确的眼表型。特定仪器包括Bioptigen 840 nm SD-OCT和Micron IV数字小动物的眼底摄像头，小动物中的验动动力学黑藻，包括大鼠，小鼠和斑马鱼和光谱SDOCT用于大型动物和人类供体的眼睛。授予保罗博士的S10赠款 Gamlin在2020年允许购买三种新工具，这些新工具将大大增强两者的眼科表型大型和小动物组织1）Zeiss Lumera 700 Resight 700操作显微镜，术中光学相干断层扫描（OCT）成像； 2）Flex模块Spectralis Oct2系统在各种体内的动物中，想象具有结构和血管造影OCT的视网膜和视神经头位置； 3）前节CASIA CASIA SS-1000扫源OCT（Tomey Corp.）用于成像角膜，虹膜，和大动物的镜头，以及小动物的整个眼睛。该核心将支持15位UAB视觉科学家，其中包括13位具有计划现代的人，以广泛的范围目前NEI R01资助。该拟议核心的董事兼副主任有广泛的出版物分别具有电视图和OCT验证/解释的经验。核心的新方向将包括一个新的基于LED的ERG系统，该系统将简化此测试。此外，一个为了形象，将重新设计用于人类视网膜成像的现有AOSLO系统啮齿动物和树在体内的眼睛。同时，荧光成像和细胞靶向的功能将添加照片刺激。此外，眼睛表型的机会将是广告以确定新的通过非眼产生的小鼠模型的全眼表型筛选的眼部小鼠模型最初生成动物的科学家回答与其器官系统有关的问题兴趣。