Assessing the accuracy of exposure measurements for common endocrine disrupting chemicals

评估常见内分泌干扰化学品暴露测量的准确性

• 批准号: 10697328
• 负责人: Patricia Hunt
• 金额: $ 15.39万
• 依托单位: WASHINGTON STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-06 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10697328
• 关键词: Address; Affect; Biological Monitoring; Blinded; Chemical Exposure; Chemicals; Collection; Coupled; Data; Dibutyl Phthalate; Dose; Endocrine Disruptors; Environmental Pollutants; Exposure to; Foundations; Funding; Glucuronides; Health; Human; Individual; Laboratories; Life; Measurement; Measures; Methodology; Methods; National Health and Nutrition Examination Survey; Ovary; Parabens; Pilot Projects; Pregnant Women; Research Design; Risk Assessment; Sampling; Second Pregnancy Trimester; Secure; Techniques; Urine; analytical method; analytical tool; bisphenol A; catalyst; cohort; epidemiologic data; epidemiology study; experimental study; exposed human population; fetal; insight; man; member; monobutyl phthalate; personal care products; safety assessment; sound

PROJECT SUMMARY Our ten-year project supporting the collection and analysis of human fetal ovaries was abruptly halted four years ago when high levels of BPA in some maternal urine samples in our cohort raised suspicion about our analytical prowess. We secured one year of R56 funding and used it to verify the accuracy of our new direct analytical method and demonstrate that older indirect methods on which human estimates of BPA exposure have been based do not accurately capture levels of the major BPA metabolite, BPA-glucuronide (BPA-G). Our data suggest human BPA exposure has been significantly underestimated. Further, because glucuronidated metabolites of other endocrine disrupting chemicals are measured using similar indirect methods, our findings raise concern that this may be a pervasive problem. The proposed pilot studies begin to address this concern by focusing on three chemicals that represent three different classes of endocrine disrupting chemicals. We will develop direct methods using authentic standards for the major metabolites of the three chemicals and use these methods to assess the accuracy of currently used indirect methods for measuring them in human biospecimens. We postulate that, like BPA, we will find inaccuracies in indirect analyses for at least two of the three. Because human biomonitoring data are used to obtain estimates of human exposure and these estimates, in turn, are essential in safety assessments, the proposed studies have the potential to affect not only human epidemiological studies designed to assess the effects of these chemicals on human health, but also to upend chemical risk assessment.

项目摘要 我们的十年项目支持人类胎儿卵巢的收集和分析突然 四年前停止了我们队列中某些母体尿液样品中的高水平BPA 对我们的分析能力提出了怀疑。我们获得了一年的R56资金并使用了 验证我们新的直接分析方法的准确性，并证明较旧的间接 人类对BPA暴露的估计的方法无法准确捕获 主要BPA代谢产物BPA-葡萄糖苷（BPA-G）的水平。我们的数据表明人BPA 暴露已被大大低估。此外，因为葡萄糖醛酸苷的代谢产物 其他内分泌干扰化学物质是使用类似间接方法测量的，我们的发现 引起人们对这可能是一个普遍的问题的担忧。拟议的试点研究开始解决 通过关注三种代表三种不同类别内分泌类的化学物质来担心这种关注 破坏化学物质。我们将使用专业的真实标准开发直接方法 三种化学物质的代谢产物，并使用这些方法评估当前的准确性 使用的间接方法用于在人类生物测量中进行测量。我们假设像BPA一样 我们将发现至少三个中的两个间接分析中的不准确性。因为人类 生物监测数据用于获得人类暴露的估计值，而这些估计又 对于安全评估至关重要，拟议的研究不仅有可能影响 人类流行病学研究旨在评估这些化学物质对人的影响 健康，也可以颠覆化学风险评估。