COLLABORATIVE PERINATAL PROJECT IN OBESITY GENOME-WIDE ASSOCIATION STUDIES

肥胖全基因组关联研究围产期合作项目

• 批准号: 10670539
• 负责人: MICHAEL TSAI
• 金额: $ 219.44万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10670539
• 关键词: 7 year old; Address; Adult; African; African American; Age; Aliquot; American; Bar Codes; Birth; Body mass index; Cardiometabolic Disease; Child; Childhood; Complex; Contractor; DNA; Enrollment; Environment; Etiology; European; Food Energy; Genetic; Genetic Predisposition to Disease; Genotype; Growth; Heritability; Life Style; Medical center; Monitor; Neonatal; Obesity; Obesity Epidemic; Pattern; Perinatal; Phenotype; Population; Pregnancy; Prenatal care; Procedures; Sampling; Serum; Ships; Standardization; Time; Umbilical Cord Blood; United States; Vial device; Woman; cohort; early childhood; early pregnancy; expectation; food consumption; genome wide association study; genomic locus; improved; infancy; lifestyle factors; milliliter; novel; obesity in children; obesogenic; offspring; physical inactivity; prevent; prospective; whole genome; 7 year old; Address; Adult; African; African American; Age; Aliquot; American; Bar Codes; Birth; Body mass index; Cardiometabolic Disease; Child; Childhood; Complex; Contractor; DNA; Enrollment; Environment; Etiology; European; Food Energy; Genetic; Genetic Predisposition to Disease; Genotype; Growth; Heritability; Life Style; Medical center; Monitor; Neonatal; Obesity; Obesity Epidemic; Pattern; Perinatal; Phenotype; Population; Pregnancy; Prenatal care; Procedures; Sampling; Serum; Ships; Standardization; Time; Umbilical Cord Blood; United States; Vial device; Woman; cohort; early childhood; early pregnancy; expectation; food consumption; genome wide association study; genomic locus; improved; infancy; lifestyle factors; milliliter; novel; obesity in children; obesogenic; offspring; physical inactivity; prevent; prospective; whole genome

Obesity is a complex multi-factorial condition that results from genetic predisposition and lifestyle factors such as high energy food consumption and physical inactivity. The heritability of childhood BMI has been estimated to be 40-70%. The high heritability of obesity in the context of the current obesogenic environment strongly suggests that genetic factors are implicated in population patterns of obesity and, are essential to consider in population approaches to address the obesity epidemic. Identifying genetic factors that influence early childhood BMI can inform the etiology of obesity as well as help to identify strategies to prevent and treat childhood obesity and related adult cardiometabolic diseases. Currently, the genetic factors that influence early childhood BMI are not well understood. Genome-wide association studies (GWAS) could help identify these genetic factors and distinguish their effects across ages. The Collaborative Perinatal Project (CPP) was a prospective pregnancy cohort that enrolled women who had prenatal care at 12 medical centers in the United States from 1959-1965. Women were followed from early pregnancy onwards and their offspring were monitored from birth through 7 years of age. While the CPP enrolled children prior to the obesity epidemic and obesogenic lifestyle in the United States, any potential interactions between genetic factors discovered using this cohort and recent environmental modifiers can be validated using other more recent cohorts. The CPP offers opportunities to discover novel genetic loci related to obesity among Africans and Europeans and to improve the functional characterization of known loci discovered in European populations. The primary aims of this study are to identify genetic influences on growth and obesityrelated phenotypes during neonatal, infancy and early childhood periods among children in the CPP cohort (n= 10200; 5100 African American and 5100 European American), and to identify trans-ancestral and ancestry-specific genetic loci associated with BMI at 7 time points from birth to 7 years of age (birth, 4 months, 8 months, 12 months, 3 years, 4 years, 7 years) via trans-ancestry GWAS. The feasibility of this project was determined in a previous pilot Task Order. The Contractor shall receive the samples over a period of approximately 8 months, with the first shipment of samples providing ample material to confirm the pilot results and standardize procedures. Samples shall arrive in their original vials, with an expectation of approximately 1 milliliter of volume per sample. The Contractor shall aliquot the volume needed for the DNA extraction and transfer any remaining volume into a new, barcoded

肥胖症是一种复杂的多因素疾病，它是由遗传易感性和生活方式因素（例如高能量食物消耗和身体不活动）所致。童年BMI的遗传力估计为40-70％。在当前肥胖环境的背景下，肥胖的高遗传力强烈表明，遗传因素与肥胖的种群模式有关，并且在人口方法中必须考虑解决肥胖症流行。 识别影响幼儿BMI的遗传因素可以告知肥胖症的病因，并有助于确定预防和治疗儿童肥胖和相关成人心脏代谢疾病的策略。目前，影响幼儿BMI的遗传因素尚不清楚。全基因组关联研究（GWAS）可以帮助识别这些遗传因素并区分它们在各个时代的影响。 合作围产期项目（CPP）是一个前瞻性怀孕队列，该孕妇从1959 - 1965年开始在美国12个医疗中心接受产前护理的妇女。妇女从怀孕初期开始受到追随，其后代从出生到7岁。在美国，CPP在肥胖流行和肥胖生活方式之前招募了儿童，但任何潜在的相互作用 在使用此队列发现的遗传因素和最近的环境修饰符之间，可以使用其他最新的同类群体进行验证。 CPP提供了发现与非洲人和欧洲人之间与肥胖有关的新型遗传基因座的机会，并提高欧洲人口中发现的已知基因座的功能表征。 The primary aims of this study are to identify genetic influences on growth and obesityrelated phenotypes during neonatal, infancy and early childhood periods among children in the CPP cohort (n= 10200; 5100 African American and 5100 European American), and to identify trans-ancestral and ancestry-specific genetic loci associated with BMI at 7 time points from birth to 7 years of age (birth, 4 months, 8 months, 12 months, 3年4年，7年）通过Trans-Accestry GWAS。 该项目的可行性是在先前的试点任务顺序中确定的。承包商应在大约8个月的时间内收到样品，首次发货提供了充足的材料以确认试点结果并标准化程序。样品应到达其原始小瓶，预计每个样品的数量约为1毫升。承包商应将DNA提取所需的体积等分，并将剩余的任何体积转移到新的条形码