Expanding regional capacity for single molecule sequencing through the purchase of the Sequel IIe sequencing system

通过购买 Sequel IIe 测序系统扩大区域单分子测序能力

• 批准号: 10632815
• 负责人: Melissa Laird Smith
• 金额: $ 48.35万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632815
• 关键词: 2019-nCoV; Area; Biological Sciences; Communities; Complex; Custom; Dedications; Development; Extramural Activities; Funding; Generations; Genomics; Goals; Growth; Immune; Infrastructure; International; Investments; Kentucky; Marketing; Methods; Minor; Phase; Preparation; Research; Research Personnel; Research Project Grants; Services; System; Technology; Universities; Viral reservoir; Work; base; genomic locus; high standard; innovation; instrument; meetings; method development; pathogen; reference genome; single molecule; single molecule real time sequencing

PROJECT SUMMARY The University of Louisville’s Sequencing Technology Center (STC) is requesting support to acquire a Pacific Biosciences Sequel IIe sequencing system. The requested system would be the second Sequel IIe system in the Center, as demand from the diverse STC user base, comprised of regional, national, and international extramurally funded investigators has far exceeded the capacity of our initial, single instrument. Since launching in 2021, the STC has assisted in the planning, preparation, and execution of many projects leveraging the power of long read, single molecule, real-time (SMRT) sequencing for projects that use both standard high fidelity (HiFi) workflows, and those that involve development of custom, innovative targeted methods to elucidate complex genomic loci or communities with phased, highly accurate long reads. Unlike other sequencing systems on the market, the Sequel IIe system has the combination of long read capabilities (150 bp – 100 kb) and the highest accuracy available from any NGS- and single molecule-sequencing instrument (ranging from Q30, 99.9% - to Q60, 99.9999%, across our projects), both of which are critical for meeting the needs of our Major, Minor, and service users. The STC is well established and already has the infrastructure and operational capabilities to implement this requested second Sequel IIe system, which is absolutely necessary for meeting the needs of our growing user base, Major User research projects, and further genomics methods development using the power of SMRT sequencing technology. The University of Louisville, particularly the Office of Research and Innovation, is dedicated to supporting the requested system through financial and infrastructure investment into the STC. From the generation of new reference genomes to targeted characterization of viral reservoirs and immune loci, and critically important SARS-CoV-2 pathogen surveillance across the Commonwealth of Kentucky, the STC facility has and will use its expertise with SMRT sequencing and the proposed new Sequel IIe system to work closely with local and national experts to facilitate cutting-edge genomics projects across a wide variety of research areas with the major goal of promoting and inspiring research and innovation in support of the growth of the research enterprise at the University of Louisville.

项目摘要 路易斯维尔大学的测序技术中心（STC）要求支持获得太平洋 生物科学续集IIE测序系统。请求的系统将是第二个续集IIE系统 该中心作为潜水员STC用户群的需求，包括区域，国家和国际 外部资助的调查人员远远超过了我们初始单仪器的能力。自推出以来 2021年，STC协助了许多项目的计划，准备和执行，以利用权力 长期读取，单分子，实时（SMRT）测序，用于使用标准高保真度（HIFI）的项目 工作流以及涉及开发自定义，创新的目标方法的工作流程，以阐明复杂 基因组本地或具有分阶段，高度准确的长读数的社区。与其他测序系统不同 市场，续集IIE系统具有长阅读功能（150 bp - 100 kb）和最高的结合 从任何NGS和单分子序列仪器可用的精度（从Q30，99.9％到范围 Q60，99.9999％在我们的项目中），这两者对于满足我们的专业，未成年人的需求至关重要 服务用户。 STC已建立得很好，并且已经具有基础架构和运营能力 实施此请求的第二续续集IIE系统，这对于满足我们的需求是绝对必要的 增长的用户基础，主要用户研究项目以及进一步的基因组方法使用能力开发 SMRT测序技术。路易斯维尔大学，特别是研究与创新办公室， 致力于通过对STC的财务和基础设施投资来支持所请求的系统。 从新的参考基因组的产生到病毒储存库和免疫位点的靶向表征， 以及在肯塔基州联邦（STC）遍布肯塔基州的至关重要的SARS-COV-2病原体监测 设施已并且将使用其专业知识与SMRT测序以及拟议的新续集IIE系统一起使用 与本地和国家专家紧密联系，以促进各种各样的尖端基因组学项目 研究领域的主要目标是促进和启发研究和创新以支持增长 路易斯维尔大学的研究企业。