Expanding regional capacity for single molecule sequencing through the purchase of the Sequel IIe sequencing system

通过购买 Sequel IIe 测序系统扩大区域单分子测序能力

• 批准号: 10632815
• 负责人: Melissa Laird Smith
• 金额: $ 48.35万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-05-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10632815
• 关键词: 2019-nCoV; Area; Biological Sciences; Communities; Complex; Custom; Dedications; Development; Extramural Activities; Funding; Generations; Genomics; Goals; Growth; Immune; Infrastructure; International; Investments; Kentucky; Marketing; Methods; Minor; Phase; Preparation; Research; Research Personnel; Research Project Grants; Services; System; Technology; Universities; Viral reservoir; Work; base; genomic locus; high standard; innovation; instrument; meetings; method development; pathogen; reference genome; single molecule; single molecule real time sequencing

PROJECT SUMMARY The University of Louisville’s Sequencing Technology Center (STC) is requesting support to acquire a Pacific Biosciences Sequel IIe sequencing system. The requested system would be the second Sequel IIe system in the Center, as demand from the diverse STC user base, comprised of regional, national, and international extramurally funded investigators has far exceeded the capacity of our initial, single instrument. Since launching in 2021, the STC has assisted in the planning, preparation, and execution of many projects leveraging the power of long read, single molecule, real-time (SMRT) sequencing for projects that use both standard high fidelity (HiFi) workflows, and those that involve development of custom, innovative targeted methods to elucidate complex genomic loci or communities with phased, highly accurate long reads. Unlike other sequencing systems on the market, the Sequel IIe system has the combination of long read capabilities (150 bp – 100 kb) and the highest accuracy available from any NGS- and single molecule-sequencing instrument (ranging from Q30, 99.9% - to Q60, 99.9999%, across our projects), both of which are critical for meeting the needs of our Major, Minor, and service users. The STC is well established and already has the infrastructure and operational capabilities to implement this requested second Sequel IIe system, which is absolutely necessary for meeting the needs of our growing user base, Major User research projects, and further genomics methods development using the power of SMRT sequencing technology. The University of Louisville, particularly the Office of Research and Innovation, is dedicated to supporting the requested system through financial and infrastructure investment into the STC. From the generation of new reference genomes to targeted characterization of viral reservoirs and immune loci, and critically important SARS-CoV-2 pathogen surveillance across the Commonwealth of Kentucky, the STC facility has and will use its expertise with SMRT sequencing and the proposed new Sequel IIe system to work closely with local and national experts to facilitate cutting-edge genomics projects across a wide variety of research areas with the major goal of promoting and inspiring research and innovation in support of the growth of the research enterprise at the University of Louisville.

项目概要 路易斯维尔大学测序技术中心 (STC) 正在请求支持以获取 Pacific Biosciences Sequel IIe 测序系统将是第二个 Sequel IIe 系统。 该中心应满足来自地区、国家和国际的不同 STC 用户群的需求 自推出以来，外部资助的研究人员远远超出了我们最初的单一仪器的能力。 2021年，STC利用权力协助了许多项目的规划、准备和执​​行 为使用标准高保真 (HiFi) 的项目提供长读长、单分子、实时 (SMRT) 测序 工作流程，以及涉及开发定制的、创新的有针对性的方法来阐明复杂的工作流程 与其他测序系统不同，具有分阶段、高精度的长读长的基因组位点或群落。 Sequel IIe 系统结合了长读取能力 (150 bp – 100 kb) 和最高的读取速度。 任何 NGS 和单分子测序仪器均可提供准确度（范围从 Q30、99.9% 到 Q60，99.9999%，在我们的项目中），这两者对于满足我们的主要、次要和 STC 已建立完善，并已具备服务用户的基础设施和运营能力。 实施这个要求的第二个 Sequel IIe 系统，这对于满足我们的需求是绝对必要的 不断增长的用户群、主要用户研究项目以及利用强大功能进一步开发基因组学方法 路易斯维尔大学，特别是研究与创新办公室， 致力于通过对 STC 的财务和基础设施投资来支持所需的系统。 从新参考基因组的生成到病毒库和免疫位点的针对性表征， 以及整个肯塔基州至关重要的 SARS-CoV-2 病原体监测，STC 该设施已经并将利用其在 SMRT 测序方面的专业知识和拟议的新 Sequel IIe 系统来发挥作用 与当地和国家专家密切合作，促进各种领域的尖端基因组学项目 主要目标是促进和激励研究与创新以支持增长的研究领域 路易斯维尔大学研究企业的负责人。