COCA: Animal & Validation Core B

古柯：动物

• 批准号: 10630225
• 负责人: Carmela M Reichel
• 金额: $ 54.4万
• 依托单位: MEDICAL UNIVERSITY OF SOUTH CAROLINA
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-15 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10630225
• 关键词: Adherence; Age; Animal Husbandry; Animal Model; Animals; Association Learning; Behavioral; Biological; Breeding; Catheters; Cells; Cocaine; Cocaine Dependence; Communication; Control Animal; Custom; Data; Databases; Drug Addiction; Drug Delivery Systems; Drug Modelings; Electrophysiology (science); Ensure; Equipment; Evaluation; Extinction; Female; Gene Expression; Generations; Genotype; Goals; Guidelines; Health; Heroin; Human Resources; IV Fluid; Individual; Infusion procedures; Institution; Knock-in; Laboratory Animals; Laboratory Rat; Measures; Mediating; Mus; National Institute of Drug Abuse; Neurobiology; Operant Conditioning; Operative Surgical Procedures; Opiate Addiction; Pharmaceutical Preparations; Phase; Plasmid Cloning Vector; Prefrontal Cortex; Privatization; Procedures; Production; Productivity; Protocols documentation; Rattus; Reagent; Recording of previous events; Reproducibility; Research; Research Personnel; Research Project Grants; Research Subjects; Resources; Rodent; Rotation; Safety; Saline; Self Administration; Services; Site; Specificity; Standardization; Sucrose; Surgical Instruments; System; Techniques; Testing; Training; Transgenes; Transgenic Animals; Transgenic Mice; Transgenic Organisms; Update; Validation; Viral; Viral Vector; Virus; Western Blotting; Withdrawal; addiction; animal resource; brain tissue; cell type; cocaine self-administration; cocaine use; confocal imaging; data dissemination; design; experience; experimental study; immunocytochemistry; in vivo calcium imaging; interest; male; mutant; personal protective equipment; pharmacologic; pre-clinical; sex; success; synergism

PROJECT SUMMARY – Animal & Validation Core B The overarching goals and responsibilities of the Animal & Validation Core B are to provide transgenic rodents with uniform histories of contingent drug delivery to the Center on Opioid and Cocaine Addiction (COCA) projects and validate the reagents and transgenic animals that are used. As such, Core B is a critical component of COCA synergy between Core C, and Projects 1-3 in the COCA by providing research subjects. The rodents include age-matched male and female rats and mice that have undergone cocaine, heroin, or sucrose self-administration and that are distributed to the projects for subsequent use according to each projects aims. Animals supplied by Core B include transgenic mice and rats for determining cell-type and circuit specificity relevant to the neurobiology of addiction. The unified Core B provides an efficient rotation of laboratory animals that creates synergy between the projects. In addition to rodents, Core B promotes synergy through the production of custom AAV viral vectors, verification of viral vector efficacy and cell specific expression, and quantitative co-localization analysis. In providing the validation services and transgenic rodents, the Animal Core B will meet the following additional objectives: 1. Maintain a high quality of technical assistance through rigorous training protocols designed with strict adherence to all institutional guidelines regarding health and safety in order to guarantee consistency of handling and ensure we are generating the healthiest subjects for our experimental purposes. 2. Establish a centralized record keeping system updated on the COCA server that aids in the transparency and dissemination of data to allow for comparisons between behavioral data and the endpoint evaluations conducted by the projects. 3. Provide a database of confirmed reagents uploaded onto the public and private COCA servers in order to identify and unify solutions for common issues associated with validation of new reagents. 4. Serve as a training center for MUSC and non-MUSC personal interested in establishing contingent models of drug delivery in their laboratories for rats and mice. 5. Become a resource for personnel needing guidance on the use of viral vector plasmids, establishing cell-type specificity, validating transgenic animals, and using quantitative colocalization techniques.

项目摘要 - 动物和验证核心B 动物和验证核心B的总体目标和责任是提供 转基因啮齿动物具有均匀的药物递送到阿片类药物中心的统一历史和 可卡因成瘾（COCA）项目并验证试剂和转基因动物 用过的。因此，核心B是核心C和项目之间可口可乐协同作用的关键组成部分 通过提供研究对象，可口可乐1-3。啮齿动物包括年龄匹配的男性和 雌性老鼠和老鼠患有可卡因，海洛因或蔗糖自我管理和 根据每个项目的目的，将其分发给项目以供随后使用。动物 核B提供的包括转基因小鼠和大鼠用于确定细胞类型和电路 与成瘾的神经生物学有关的特异性。统一的核心B提供了有效的效率 在项目之间创造协同作用的实验动物的旋转。除啮齿动物外， 核心B通过生产定制的AAV病毒向量促进协同作用，验证 病毒载体效率和细胞特异性表达以及定量共定位分析。在 提供验证服务和转基因啮齿动物，动物核心B将满足 以下其他目标： 1。通过设计的严格培训协议维持高质量的技术援助 严格遵守有关健康和安全的所有机构指南，以便 保证处理的一致性并确保我们为自己的最健康的主题产生最健康的主题 实验目的。 2。建立在可口服务器上更新的集中记录，以帮助 数据的透明度和传播，以允许行为数据和 项目进行的终点评估。 3。提供上传到公共和私人可口的确认试剂的数据库 服务器以识别和统一解决与验证相关的常见问题的解决方案 新试剂。 4。作为MUSC和非MUSC个人兴趣的培训中心 在其实验室的大鼠和小鼠实验室的药物输送模型。 5。成为需要有关使用病毒载体质粒的指导的人员的资源， 建立细胞类型的特异性，验证转基因动物并使用定量 共定位技术。