Novel Sensor Integrated Proteome on Chip (SPOC) platform for evaluating kinetic parameters of protein interactions in high throughput

新型传感器集成芯片上蛋白质组 (SPOC) 平台，用于评估高通量蛋白质相互作用的动力学参数

• 批准号: 10547479
• 负责人: Bharath Takulapalli
• 金额: $ 117.87万
• 依托单位: SPOC PROTEOMICS INC.
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-08-12 至 2024-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10547479
• 关键词: Address; Animal Model; Antibodies; Area; Back; Benchmarking; Binding; Binding Proteins; Biological Assay; Biological Process; Biosensor; Budgets; Capital; Catalogs; Cells; ChIP-on-chip; Collaborations; Custom; DNA; Data; Detection; Development; Diagnosis; Enzyme-Linked Immunosorbent Assay; Evaluation; Florida; Fluorescent Dyes; Funding; Genes; Goals; Gold; Human; Image; Imaging Device; In Situ; Institutes; Investments; Kinetics; Label; Length; Letters; Microarray Analysis; Molecular; Monitor; Monoclonal Antibodies; Mus; Natural regeneration; Nature; Output; Pattern; Phage Display; Phase; Privatization; Protein Array; Protein Microchips; Proteins; Proteome; Proteomics; Protocols documentation; RNA; Research; Running; Serum; Shapes; Small Business Innovation Research Grant; Spottings; Surface; Surface Plasmon Resonance; System; Technology; Testing; Time; Validation; Variant; base; biomarker discovery; clinically relevant; commercialization; cost; drug development; high throughput analysis; imaging detection; imaging system; instrument; monolayer; mouse model; novel; phenotypic biomarker; precision medicine; programs; protein expression; protein folding; protein function; protein protein interaction; screening; sensor; small molecule; testing services; tool; vaccine development; waiver

Abstract A critical bottleneck in the field of proteomics is the inability to simultaneously and cost-effectively evaluate thousands of functional protein interactions at once to obtain kinetic data. Although conventional in-situ protein microarray technology is well developed, it uses fluorescent dye based detection that provides only qualitative or semi-quantitative information. There is currently no technology that offers label-free real-time interaction assays of full-length folded proteins in high throughput format. To address this unmet need, INanoBio is developing novel sensor integrated proteome on chip (SPOC) platform that combines in situ expression and capture of functional proteins on biosensors, to allow instant high throughput analysis of thousands of proteins in real time. In this SBIR phase II project, we propose to develop SPOC platform by integrating in situ produced protein arrays with surface plasmon resonance imaging (SPRi), by building integrated ProSPR instrument that will enable SPRi determination of both qualitative and kinetic information of protein biomolecular interactions in high throughput. We will develop, validate and apply 1000 protein SPOC SPRi sensor chips to study clinically relevant protein- protein interactions and test off-target binding of new antibodies produced in animal models against human proteins.

抽象的 蛋白质组学领域的关键瓶颈是无法同时且具有成本效益 立即评估数千种功能性蛋白质相互作用以获取动力学数据。虽然 常规的原位蛋白微阵列技术发达了，它使用荧光染料 仅提供定性或半定量信息的基于检测。目前有 没有技术可提供无标签的实时互动测定法的全长折叠蛋白 高通量格式。为了满足这种未满足的需求，inanobio正在开发新型传感器 芯片（SPOC）平台上的集成蛋白质组，结合原位表达和捕获 生物传感器上的功能蛋白，以允许即时高通量分析数千个 实时蛋白质。在这个SBIR II期项目中，我们建议通过 通过表面等离子体的原位产生蛋白质阵列与表面等离子体共振成像（SPRI）。 构建综合ProSPR工具，该工具将能够确定定性和 高吞吐量中蛋白质生物分子相互作用的动力学信息。我们将发展， 验证并应用1000蛋白SPOC SPRI SENSOR芯片来研究临床相关的蛋白质 蛋白质相互作用和测试动物模型中产生的新抗体的靶向靶向结合 反对人类蛋白质。