Cellular Immunology Metabolism Core

细胞免疫学代谢核心

• 批准号: 10664046
• 负责人: AUGUSTO C. OCHOA
• 金额: $ 10.62万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10664046
• 关键词: Affect; Aging; Autophagocytosis; Biological Assay; Biological Markers; Biological Response Modifiers; Biopsy; Cancer Center; Cell Culture Techniques; Cell Death; Cell Proliferation; Cell Separation; Cell Survival; Cell-Mediated Cytolysis; Cells; Cellular Immunology; Centers of Research Excellence; Collaborations; Color; Communities; Cytometry; Data; Detection; Development; Disease; Drug Delivery Systems; Drug Evaluation; Effectiveness; Energy Metabolism; Environment; Equipment; Evaluation; Fatty Acids; Flow Cytometry; Frequencies; Funding; Funding Mechanisms; Gene Expression; Genomics; Genus Hippocampus; Goals; Growth; Health system; High Pressure Liquid Chromatography; Human Resources; Hypoxia; Image; Immunoassay; Immunologic Markers; Immunology; Immunosuppression; Inflammation; Infrastructure; Institution; Internet; Laboratories; Laboratory Personnel; Link; Louisiana; Malignant Neoplasms; Mediation; Mentors; Messenger RNA; Metabolic; Metabolism; Mitochondria; Mitogens; NCI Center for Cancer Research; Nanotechnology; Nitrogen; Oncology; Oxygen; Oxygen Consumption; Participant; Pathway interactions; Phase; Policies; Population; Positioning Attribute; Principal Investigator; Production; Proliferating; Protocols documentation; Research; Research Personnel; Research Project Grants; Research Support; Running; Services; Signal Transduction; Social Distance; Speed; Technology; Teleconferences; Time; Tissues; Training; Translational Research; Universities; Update; Viral; Virus; Work; anticancer research; cancer stem cell; cell growth; cytokine; effector T cell; equipment training; experimental study; glucose uptake; immune activation; improved; innovation; innovative technologies; instrument; instrumentation; medical schools; meetings; metabolomics; microvesicles; nanoparticle; neoplastic cell; new technology; novel; novel therapeutics; response; single cell technology; social media; stem cell biomarkers; stemness; translational scientist; tumor; tumor metabolism; tumor microenvironment; tumorigenesis; virus related cancer

ABSTRACT The Cellular Immunology and Metabolism Core (CIMC) of the LSU-Cancer Center was created in 2006 with the objective of providing flow cytometry support to Junior Principal Investigators (JPIs) funded during the Phase I COBRE “Mentoring Translational Researchers in Louisiana” (MTRL). During Phase II and Phase III, CIMC evolved to provide innovative technologies not only to COBRE- supported JPIs, but also to the LSUHSC investigators and to other users in our region. Since 2017, the CIMC has been partially supported by the Phase I COBRE “Center for Translational Viral Oncology (CTVO) and extensively used by CTVO JPIs. We will continue that support In Phase 2 CTVO and further expand our services for JPIs. Current CIMC laboratories, now, provide comprehensive analytic flow cytometry and high-speed cell sorting services and functional assays including mitogen-induced cell proliferation, cytokine production, cell death and survival, cell mediated cytotoxicity, effector T cell frequency, and the detection of immune mediators through multiplex approaches. During Phase I of the CTVO, CIMC expanded infrastructure and expertise to analysis of cellular metabolism, including oxygen consumption and acidification rates, and mitochondrial function in both normoxic and hypoxic environments. These additional CIMC services will now support the needs of CTVO Phase II JPIs in at least 3 Research Projects. This will allow for expansion of JPI expertise in identifying new biomarkers of virus-induced cancers and evaluation of cell growth and cell death responses associated with viral oncogenesis and the tumor microenvironment. In addition, innovative approaches, are continuously evolving in the CIMC personnel, and will be made available to JPIs and their mentors for developing new experimental strategies to explore virus-induced changes at the levels of tumor cell signaling, and tumor cell energy metabolism. These advanced technologies have positioned the CIMC as the leading laboratory in cancer and immunology in the region, resulting in increased users, including CTVO JPIs, as well as CIMC integration as a core laboratory for the LSUHSC School of Medicine, and LSU and Tulane Cancer Centers co-located in the Louisiana Cancer Research Center (LCRC).

抽象的 LSU-CASTER中心的细胞免疫学和代谢核心（CIMC）是在 2006年的目的是为初级首席研究人员（JPI）提供流式细胞仪支持 在第一阶段的Cobre资金“指导路易斯安那州的转化研究人员”（MTRL）。 在II阶段和III期期间，CIMC不仅提供创新的技术 支持JPI，也支持LSUHSC调查人员和我们地区的其他用户。自2017年以来 CIMC已得到了I期COBRE“翻译病毒肿瘤中心的部分支持 （CTVO）并由CTVO JPI广泛使用。我们将继续在第2阶段CTVO和 进一步扩大我们为JPI的服务。当前的CIMC实验室现在提供了全面的分析 流式细胞仪和高速细胞分选服务以及功能测定法，包括有丝分裂原诱导的 细胞增殖，细胞因子产生，细胞死亡和存活，细胞介导的细胞毒性，效应T细胞 频率和通过多重方法检测免疫介质。在第一阶段 CTVO，CIMC扩大了基础设施和专业知识，以分析细胞代谢，包括 氧气和酸化速率，以及在常氧和低氧中的线粒体功能 环境。这些额外的CIMC服务现在将支持CTVO II阶段JPI的需求 至少3个研究项目。这将允许扩大JPI专业知识来识别新的 病毒诱导的癌症的生物标志物以及对相关细胞生长和细胞死亡反应的评估 具有病毒肿瘤生成和肿瘤微环境。此外，创新的方法是 在CIMC人员中不断发展，将为JPI及其导师提供 制定新的实验策略来探索病毒诱导的肿瘤细胞水平的变化 信号传导和肿瘤细胞能代谢。这些高级技术已将CIMC定位 作为该地区癌症和免疫学领域的主要实验室，导致使用者增加， 包括CTVO JPI，以及CIMC集成作为LSUHSC学校的核心实验室 医学，路易斯安那州癌症研究中心同期的LSU和图兰癌中心 （LCRC）。