Funtion of Uncharacterized Genes of Acinetobacter baumanii

鲍曼不动杆菌未表征基因的功能

• 批准号: 8688145
• 负责人: Samuel I Miller
• 金额: $ 161.53万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-06-24 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8688145
• 关键词: Acinetobacter; Acinetobacter baumannii; Antibiotic Resistance; Antibiotics; Antimicrobial Resistance; Bacteria; Biochemical; Biocide; Cell Membrane Permeability; Cell surface; Code; Communicable Diseases; Data; Desiccation; Development; Disinfectants; Disinfection; Drug Targeting; Drug resistance; Enzymes; Genes; Genetic; Genome; Genomics; Goals; Hospitals; Individual; Infection; Knowledge; Modeling; Molecular; Multi-Drug Resistance; Natural Immunity; Nosocomial Infections; Opportunistic Infections; Organism; Pathogenesis; Pharmaceutical Preparations; Phenotype; Process; Proteins; Proteomics; Research; Research Proposals; Resistance; Resources; Role; Surface Properties; Technology; Testing; Therapeutic; Work; antimicrobial; base; biological adaptation to stress; clinically relevant; data management; efflux pump; environmental change; gene function; genetic analysis; human disease; mutant; novel strategies; pathogen; programs; public health relevance; relational database; response; screening; trait

DESCRIPTION (provided by applicant): Acinetobacter baumannii (Ab) is an emerging pathogen that causes primary and opportunistic infections, and is a serious concern in the hospital and battlefield setting. This organism has significant intrinsic resistance to antibioticsas well as an ability to quickly acquire new resistance. Additionally, Ab can persist in a desiccated state on fomites, making disinfection and management of nosocomial infections particularly troublesome. The goal of this research proposal is to find unexploited therapeutic approaches by elucidating the role of uncharacterized genes of Ab in this organism's mechanisms of pathogenesis, resistance, and persistence. Three projects will be established to 1) use a genomic approach to broadly identify the role of uncharacterized genes (Colin Manoil, Project 1 Director) 2) determine the role of envelope integrity with regard to bacterial viability (Sam Mille, PI/PD, Project 2 Director), and use expression analysis to identify genes and sRNAs of unknown function associated with drug, disinfectant, and desiccation tolerance (Carrie Harwood, Project 3 Director). The work of the Center will be supported by an Administrative Core (Core 1, John Kemner Core Leader), a Protein Interaction Technology Core (Jim Bruce Core 2 Director Leader), and a Data Dissemination/Resource Management Core (Mitch Brittnacher, Core 3 Director).

描述（由申请人提供）：鲍曼不动杆菌 (Ab) 是一种新出现的病原体，可引起原发性感染和机会性感染，在医院和战场环境中是一个严重问题。这种生物体对抗生素具有显着的内在耐药性，并且能够快速获得新的耐药性。此外，抗体可以在污染物上以干燥状态持续存在，使得医院感染的消毒和管理特别麻烦。 本研究计划的目标是通过阐明未表征的抗体基因在该生物体的发病机制、抵抗力和持久性机制中的作用来找到尚未开发的治疗方法。 将建立三个项目：1) 使用基因组方法广泛识别未表征基因的作用（Colin Manoil，项目 1 主任）2) 确定包膜完整性对细菌活力的作用（Sam Mille，PI/PD，项目2 主任），并使用表达分析来识别与药物、消毒剂和干燥耐受性相关的未知功能的基因和 sRNA（Carrie Harwood，项目 3 主任）。该中心的工作将得到行政核心（核心 1，John Kemner 核心领导者）、蛋白质相互作用技术核心（Jim Bruce 核心 2 主任领导者）和数据传播/资源管理核心（Mitch Brittnacher，核心 3导演）。