Interactive, Self-Paced Training Modules for Cryo-EM and Cryo-ET Novices

针对 Cryo-EM 和 Cryo-ET 新手的交互式自定进度培训模块

• 批准号: 10662452
• 负责人: Julia Brasch
• 金额: $ 12.42万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-05-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10662452
• 关键词: 3-Dimensional; Address; Area; Award; Biological; Cells; Certification; Competence; Complement; Creativeness; Cryo-electron tomography; Cryoelectron Microscopy; Data; Data Collection; Dedications; Development; Educational Curriculum; Educational process of instructing; Electronic Mail; Equipment; Feedback; Freezing; Goals; Home; Image; Institution; Knowledge; Learning; Learning Module; Licensing; Life; Methods; Modeling; Multimedia; Negative Staining; Online Systems; Performance; Preparation; Productivity; Proteins; Reagent; Resolution; Resource Sharing; Resources; Sampling; Self Assessment; Side; Sister; Site Visit; Specimen; Structure; Surveys; Testing; Time; Training; Training Activity; Training Programs; United States National Institutes of Health; Universities; Utah; Visual; Visualization; animation; cost; design; empowerment; expectation; experience; hands-on learning; image processing; improved; instrument; instrumentation; meetings; member; online course; programs; response; screening; skills; small molecule; social media; three-dimensional modeling; tool; user-friendly; web site; working group

Project Summary/Abstract Cryo-EM and cryo-ET have emerged as indispensable tools to visualize biological structures across a broad range of scales, from small molecules and proteins to entire cells. Despite the growing enthusiasm for these methods, cryo-EM and cryo-ET are relatively inaccessible for several reasons: first, there are inadequate training materials for cryo-EM and cryo-ET novices; second, there is a growing expertise gap between experienced practitioners and newcomers; and third, few institutions have the means to procure and maintain the high cost of instrumentation. In order to address these limitations, we created ‘CryoEM 101’, a self-paced, interactive online course that uses a user-friendly and media-rich approach to engage and teach newcomers on the basics of the typical cryo-EM project workflow. We now propose to expand CryoEM 101 by developing new content that emphasize best practices in sample and grid preparation, creating self-assessment quizzes for users to gauge their learning, and integrating our training material with a broader “merit badge” certification program that is currently under development. We also propose to create CryoET 101, which will be modeled after CryoEM 101 by using video, 3D animation, and interactive modules to teach the principles and best practices underlying cryo-ET. Like CryoEM 101, CryoET 101 will be divided into several chapters, each of which will focus on a distinct step along the cryo-ET workflow and will contain visually engaging modules that teach core principles. The accuracy and quality of the training material will be evaluated by separate Advisory Boards for cryo-EM and cryo-ET that consist of subject experts in each method. User proficiency will be assessed through end-of-chapter and end-of-course quizzes. Overall, the goal of this proposal is to guide cryo- EM and cryo-ET newcomers to a point along their training where they can independently prepare and optimize their specimens for high-quality data collection at dedicated EM centers.

项目摘要/摘要 Cryo-EM和Cryo-Et已成为必不可少的工具，可将生物结构可视化 从小分子和蛋白质到整个细胞的广泛尺度范围。尽管对 这些方法，Cryo-EM和Cryo-Et相对无法访问，原因是几个原因：首先，存在不足 冷冻EM和低温网小说的培训材料；其次，之间越来越多的专业知识差距 经验丰富的从业者和新移民；第三，很少有机构能够采购和维护 仪器的高成本。为了解决这些局限 互动在线课程，使用用户友好且媒体丰富的方法吸引和教授新移民 在典型的Cryo-EM项目工作流程的基础上。我们现在建议通过开发来扩展冷冻101 强调样品和电网准备中最佳实践的新内容，创建自我评估测验 用户可以评估他们的学习，并将我们的培训材料与更广泛的“优异徽章”认证整合在一起 目前正在开发的程序。我们还建议创建Cryoet 101，将建模 在使用视频，3D动画和交互式模块来教授原理和最佳的互动模块之后 练习低温-ET。像冷冻101一样，冷冻101将分为几章 它将集中于沿着冷冻工作流程的独特步骤，并包含视觉引人入胜的模块 教核心原则。培训材料的准确性和质量将通过单独的咨询评估 Cryo-EM和Cryo-ET的板由每种方法中的主题专家组成。用户的能力将是 通过章节结束和课程结束测验进行评估。总体而言，该提议的目的是指导冷冻 EM和Cryo-Et的新移民到他们的培训中，他们可以独立准备和优化 他们在专用EM中心收集高质量数据的标本。