IMAGING OPTIC NERVE FUNCTION AND PATHOLOGY

视神经功能和病理学成像

基本信息

批准号：
8912809
负责人：
SHENG-KWEI SONG
金额：
$ 64.11万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-05-01 至 2020-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8912809
关键词：
Acute Affect Anatomy Anterior Axon Brain Computer Simulation Data Demyelinations Detection Development Diffusion Diffusion Magnetic Resonance Imaging Disease Distal Distant Etiology Eye Functional Magnetic Resonance Imaging Glaucoma Goals Health Histology Human Image Imaging Techniques In Situ Inflammation Injury Investigation Light Measurement Measures Methodology Methods Microscopic Modality Monitor Monte Carlo Method Mus Natural regeneration Nerve Nerve Crush Neurons Optic Chiasm Optic Nerve Optic Neuritis Optic tract structure Optical Coherence Tomography Pathology Patients Photic Stimulation Play Protocols documentation Role Sampling Scanning Signal Transduction Structure Techniques Technology Time Universities Variant Vision Visual Acuity Visual Pathways Visual system structure Washington axon injury base follow-up functional status human subject imaging modality improved in vivo medical schools mouse model neurotransmission novel optic nerve disorder public health relevance relating to nervous system research study response spectrograph three dimensional structure transmission process

项目摘要

DESCRIPTION (provided by applicant): The stated aspirational goal of the NEI, to Regenerate Neurons and Neural Connections in the Eye and Visual System, requires the development of modalities capable of non-invasively imaging neural connections as they are reestablished between the eye and the brain. In proof-of-concept studies we have introduced two promising techniques, diffusion basis spectrum imaging (DBSI) and diffusion functional magnetic resonance imaging (diffusion fMRI) for visualizing the pathology and function of the optic nerve in situ. In the current proposal, we will combine these technologies to deliver a new, diffusion MRI-based method to assess optic nerve anatomy, function and pathology simultaneously in both mice and human subjects. We will validate this approach by monitoring the progression and/or regression of axonal damage in glaucoma and optic neuritis. In keeping with the overall aspirations of the NEI, our long-term goal is to utilize this methodology to assess, non- invasively, the structure and function of regenerating axons in the optic nerve. Three specific aims will be pursued: (1) To quantify the relationships between diffusion MRI signals, axon number and visual function in an optic nerve crush mouse model, correlating DBSI with histological counts of axon number and diffusion fMRI with visual acuity; (2) To perform in vivo experiments and in silico computation (adapting structural information obtained from histology) on the optic nerve crush mouse model to identify a diffusion time optimized for both DBSI and diffusion fMRI and thus distinguish the contribution of restricted isotropic (distant from the axons) and anisotropic (adjacent to the axons) diffusion; and (3) To develop and optimize in vivo human optic nerve diffusion MRI protocol and visual stimulation paradigm that can simultaneously visualize optic nerve anatomy, function and pathology in glaucoma and optic neuritis patients. At completion, we will have established a novel imaging method to simultaneously assess optic nerve anatomy, function, and pathology allowing a detailed pathophysiological investigation of optic neuropathies.

描述（由申请人提供）：NEI 的既定目标是在眼睛和视觉系统中再生神经元和神经连接，需要开发能够在眼睛和视觉系统之间重建神经连接时对神经连接进行非侵入性成像的模式。在概念验证研究中，我们引入了两种有前途的技术，即扩散基础光谱成像（DBSI）和扩散功能磁共振成像（扩散fMRI），用于可视化光学的病理和功能。在当前的提案中，我们将结合这些技术来提供一种新的基于扩散 MRI 的方法，以同时评估小鼠和人类受试者的视神经解剖结构、功能和病理学。我们将通过监测进展来验证这种方法。和/或青光眼和视神经炎轴突损伤的消退为了与 NEI 的总体愿望保持一致，我们的长期目标是利用这种方法来非侵入性地评估视神经再生轴突的结构和功能。我们将追求三个具体目标：（1）量化视神经挤压小鼠模型中扩散 MRI 信号、轴突数量和视觉功能之间的关系，将 DBSI 与轴突数量的组织学计数和扩散 fMRI 与视力相关联； ) 在视神经挤压小鼠模型上进行体内实验和计算机计算（调整从组织学获得的结构信息），以确定针对 DBSI 和扩散 fMRI 优化的扩散时间，从而区分限制各向同性（距离 (3) 开发和优化体内人视神经扩散 MRI 协议和视觉刺激范例，可同时可视化青光眼和视神经炎患者的视神经解剖结构、功能和病理学完成后，我们将建立一种新颖的成像方法来同时评估视神经解剖结构、功能和病理学，从而对视神经病变进行详细的病理生理学研究。