Core E: Cellular and Organismic Systems for Antiviral Testing

核心 E：用于抗病毒测试的细胞和有机体系统

基本信息

批准号：
10522809
负责人：
Ryan Langlois
金额：
$ 845.18万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-16 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10522809
关键词：
2019-nCoV 3&apos -nuclease Address Animal Disease Models Animal Model Animal Testing Animals Antiviral Agents Arenavirus Back Biochemical Biological Models Bolivian Hemorrhagic Fever Virus Boston Cell Line Cell model Cell physiology Cells Clinical Containment Coronavirus Data Dose Drug Kinetics Ebola Ebola Hemorrhagic Fever Ebola virus Evaluation Filovirus Flavivirus Hematological Disease Human Human Resources Immunology Iowa Lassa virus Lead Licensing Methods Microbiology Midwestern United States Minnesota Modeling Monoclonal Antibodies Oral Organism Outcome Study Pathogenesis Peptide Hydrolases Performance Pharmaceutical Preparations Pharmacotherapy Property Prophylactic treatment Research Design Research Personnel Resources Rodent Model Scientist Standardization System Technical Expertise Testing Time Tissues Treatment Efficacy Universities Viral Viral load measurement Virus Virus Diseases Zika Virus anti-viral efficacy base drug candidate drug development efficacious intervention experience experimental study graduate student helicase high throughput screening human disease in vivo interest intraperitoneal meetings mid-career faculty nanobodies novel pandemic disease pathogen prevent prophylactic respiratory virus response small molecule small molecule therapeutics therapeutic evaluation virology

项目摘要

Core E – Cellular and Organismic Systems for Antiviral Testing Summary The Cellular and Organismic Systems Core supports the entire Midwest AViDD Center by coordinating and implementing cell and animal antiviral testing experiments required to address the aims in each of the Projects. A Cellular and Organismic Systems Core group is necessary for this AViDD Center because of the need for consistency in determining the antiviral activity of each compound, and because of the intense requirements for handling pathogens that require high containment. Based at the University of Minnesota, Boston University and the University of Iowa, the Cellular and Organismic Systems Core provides the specialized resources and administrative framework for performing cellular and animal BSL2, BSL3, BSL4 studies. Core E will focus on SARS-CoV-2, Zika virus, Ebola virus and Lassa virus. This core will evaluate validated hits through high throughput screening in Aim 1. Successful compounds will then go back to the projects and cores for further refinement. Aim 2 will then evaluate refined compounds for antiviral activity and dose response, the potential for escape, and efficacy in primary human cells. Finally, compounds that demonstrate efficacy in Aim 2 will be evaluated for prophylactic and therapeutic efficacy in relevant animal disease models in Aim 3.

核心 E – 用于抗病毒测试的细胞和有机体系统概括细胞和有机系统核心通过协调和支持整个中西部 AViDD 中心实施实现每个项目目标所需的细胞和动物抗病毒测试实验。该 AViDD 中心需要一个细胞和有机体系统核心小组，因为需要确定每种化合物的抗病毒活性的一致性，并且由于对处理需要高度控制的病原体总部位于明尼苏达大学、波士顿大学和爱荷华大学细胞和有机系统核心提供专业资源和执行细胞和动物 BSL2、BSL3、BSL4 研究的管理框架将重点关注核心 E。该核心将通过高效率评估 SARS-CoV-2、寨卡病毒、埃博拉病毒和拉沙病毒。目标 1 中的通量筛选。成功的化合物将返回到项目和核心进行进一步的研究然后，目标 2 将评估精制化合物的抗病毒活性和剂量反应以及潜在的作用。最后，在 Aim 2 中表现出功效的化合物将是。评估目标 3 中相关动物疾病模型的预防和治疗效果。