Core E: Cellular and Organismic Systems for Antiviral Testing

核心 E：用于抗病毒测试的细胞和有机体系统

基本信息

批准号：
10522809
负责人：
Ryan Langlois
金额：
$ 845.18万
依托单位：
UNIVERSITY OF MINNESOTA
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-05-16 至 2025-04-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10522809
关键词：
2019-nCoV 3&apos -nuclease Address Animal Disease Models Animal Model Animal Testing Animals Antiviral Agents Arenavirus Back Biochemical Biological Models Bolivian Hemorrhagic Fever Virus Boston Cell Line Cell model Cell physiology Cells Clinical Containment Coronavirus Data Dose Drug Kinetics Ebola Ebola Hemorrhagic Fever Ebola virus Evaluation Filovirus Flavivirus Hematological Disease Human Human Resources Immunology Iowa Lassa virus Lead Licensing Methods Microbiology Midwestern United States Minnesota Modeling Monoclonal Antibodies Oral Organism Outcome Study Pathogenesis Peptide Hydrolases Performance Pharmaceutical Preparations Pharmacotherapy Property Prophylactic treatment Research Design Research Personnel Resources Rodent Model Scientist Standardization System Technical Expertise Testing Time Tissues Treatment Efficacy Universities Viral Viral load measurement Virus Virus Diseases Zika Virus anti-viral efficacy base drug candidate drug development efficacious intervention experience experimental study graduate student helicase high throughput screening human disease in vivo interest intraperitoneal meetings mid-career faculty nanobodies novel pandemic disease pathogen prevent prophylactic respiratory virus response small molecule small molecule therapeutics therapeutic evaluation virology

项目摘要

Core E – Cellular and Organismic Systems for Antiviral Testing Summary The Cellular and Organismic Systems Core supports the entire Midwest AViDD Center by coordinating and implementing cell and animal antiviral testing experiments required to address the aims in each of the Projects. A Cellular and Organismic Systems Core group is necessary for this AViDD Center because of the need for consistency in determining the antiviral activity of each compound, and because of the intense requirements for handling pathogens that require high containment. Based at the University of Minnesota, Boston University and the University of Iowa, the Cellular and Organismic Systems Core provides the specialized resources and administrative framework for performing cellular and animal BSL2, BSL3, BSL4 studies. Core E will focus on SARS-CoV-2, Zika virus, Ebola virus and Lassa virus. This core will evaluate validated hits through high throughput screening in Aim 1. Successful compounds will then go back to the projects and cores for further refinement. Aim 2 will then evaluate refined compounds for antiviral activity and dose response, the potential for escape, and efficacy in primary human cells. Finally, compounds that demonstrate efficacy in Aim 2 will be evaluated for prophylactic and therapeutic efficacy in relevant animal disease models in Aim 3.

核心E - 用于抗病毒测试的细胞和有机系统概括细胞和有机系统核心通过协调和实施细胞和动物抗病毒测试实验，以解决每个项目中的目标。由于需要确定每种化合物的抗病毒活性的一致性，并且由于对处理需要高遏制的病原体。位于明尼苏达大学，波士顿大学和爱荷华大学，蜂窝和有机系统核心提供专业资源和用于执行细胞和动物BSL2，BSL3，BSL4研究的行政框架。核心E将重点关注 SARS-COV-2，寨卡病毒，埃博拉病毒和LASSA病毒。该核心将通过高级评估验证的命中 AIM 1中的吞吐量筛查。成功的化合物将返回项目和核心以进一步精致。然后，AIM 2将评估精制化合物的抗病毒活性和剂量反应，其潜力逃脱和原代人细胞的效率。最后，证明AIM 2中效率的化合物将是在AIM 3中评估了相关动物疾病模型中的预防和治疗效率。