Xenopus Mutant Resource

非洲爪蟾突变体资源

• 批准号: 10646475
• 负责人: Marko E Horb
• 金额: $ 74.01万
• 依托单位: MARINE BIOLOGICAL LABORATORY
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10646475
• 关键词: Agreement; Amphibia; Anatomy; Animals; Avidin; Biological Models; Biomedical Research; Biotin; Breeding; Cellular biology; Clustered Regularly Interspaced Short Palindromic Repeats; Collaborations; Communities; DNA; Data; Destinations; Development; Developmental Biology; Diploidy; Disease; Embryo; Emerging Technologies; Generations; Genes; Genetic; Genetic Models; Goals; Grant; Heritability; Human; Individual; Infrastructure; Injections; Investments; Knock-out; Label; Methods; Modeling; Neurobiology; Oocytes; Organogenesis; Paper; Pathway interactions; Physiology; Proteins; Research; Research Personnel; Resources; Scientist; Signal Transduction; System; Testing; Time; Tissues; Toes; Training; Transgenic Organisms; United States National Institutes of Health; Variant; Veins; Vitellogenins; Work; Xenopus; Xenopus laevis; biomedical resource; experimental study; genome editing; human disease; human model; insight; meetings; molecular mechanics; mutant; new technology; repaired; response; site-specific integration; uptake; water quality; xenopus genome

Project Summary This grant supports a project to generate and characterize Xenopus mutants in key genes related to human disease, establish a new Xenopus Mutant Resource, develop new transgenic lines for genome editing and establish efficient site-specific integration. The long-term goals of the studies are to generate new models of human disease that will provide useful resources for the biomedical research community. The proposed studies will use CRISPR-Cas gene editing to create precise models of human disease in the amphibian Xenopus. The current studies are focused on developing Xenopus models of many different diseases for the entire Xenopus community, and each mutant will be developed in close coordination with individual researchers. There are four main aims to this proposal. First, we propose to collaborate with Xenopus researchers to characterize existing mutants (over 116) that were developed over the last four years. The mutants cover a wide range of topics that require varied expertise that can only be obtained through interactions with other researchers. Second, we propose to generate new mutants using CRISPR-Cas as requested by researchers. As the national stock center for Xenopus, we have the proven expertise to breed and maintain these mutants. Third, we will generate new transgenic lines that will be used for genome editing. These new lines will allow for more tissue-specific expression of Cas9. Fourth, we propose to develop homology directed repair for more efficient generation of site-specific integration of exogenous DNA. All of these aims will help enhance the utilization of CRISPR-Cas gene editing methods in the Xenopus model system.

项目摘要 该赠款支持一个项目，以生成和表征与人类有关的关键基因中的xenopus突变体 疾病，建立新的爪蟾突变资源，开发用于基因组编辑和的新转基因线 建立有效的特定地点集成。研究的长期目标是生成新的模型 人类疾病将为生物医学研究界提供有用的资源。提议 研究将使用CRISPR-CAS基因编辑来创建两栖动物的精确模型 Xenopus。当前的研究重点是开发许多不同疾病的爪蟾模型 整个Xenopus社区以及每个突变体将与个人密切协调开发 研究人员。该提案有四个主要目标。首先，我们建议与Xenopus合作 研究人员表征了过去四年中现有的突变体（超过116）。这 突变体涵盖了需要各种专业知识的广泛主题，只能通过 与其他研究人员的互动。其次，我们建议使用CRISPR-CAS生成新的突变体 由研究人员要求。作为全国纳博斯股票中心，我们拥有良好的专业知识来繁殖 并保持这些突变体。第三，我们将生成新的转基因线，用于基因组编辑。 这些新线条将允许CAS9的更多组织特异性表达。第四，我们建议发展 同源性修复，以更有效地生成外源性DNA的位点特异性整合。所有人 这些目标将有助于增强Xenopus模型中CRISPR-CAS基因编辑方法的利用 系统。