Understanding the role of the frontal cortex during cognitive flexibility in Fmr1 knock outs

了解 Fmr1 敲除中额叶皮层在认知灵活性中的作用

基本信息

批准号：
10647381
负责人：
Bethany Plakke Anderson
金额：
$ 7.18万
依托单位：
KANSAS STATE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-03-01 至 2025-02-28
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10647381
关键词：
Affect Animals Anterior Attention Brain Categories Cognition Cognitive Cognitive deficits Cues Data Dimensions Electroencephalography Exhibits FMR1 Face Female Fragile X Syndrome Frequencies Future Genetic Genetic Models Genetic Risk Glutamate Receptor Goals Human Impairment Individual Intellectual functioning disability Interneurons Knock-out Learning Left Measures Medial Methods Mission Modeling National Institute of Child Health and Human Development Neurodevelopmental Disorder Neurophysiology - biologic function Outcome Pathology Perception Performance Persons Phase Prefrontal Cortex Publications Pyramidal Cells Rattus Research Personnel Reversal Learning Role Rotation Side Stimulus Syndrome Time Training Valproic Acid Work autism spectrum disorder behavioral phenotyping cingulate cortex cognitive function cognitive task developmental disease flexibility frontal lobe human data improved in vivo individuals with autism spectrum disorder male neural neurophysiology receptor function touchscreen

项目摘要

PROJECT SUMMARY Autism spectrum disorder (ASD) is a neurodevelopmental disorder where individuals exhibit deficits in cognitive flexibility. Purpose: This proposal will record from the frontal cortex of Fmr1 KOs (model of Fragile X syndrome) during cognitive flexibility tasks and assess how neural hyperexcitability within the KOs impacts reversal learning and shifts of attention. Methods: Fmr1 KOs and wildtype rats will be trained on categorical perception tasks with touchscreens in operant chambers. Single-unit neurophysiology and local field potentials will be collected from the frontal cortex (including the anterior cingulate) during the tasks. It is important to understand how altered neural function within the KOs impacts cognitive function and learning. This project will compare neural activity from putative pyramidal and interneurons to examine differential functions during cognition. Examining these differences in neural function between KOs and WT will enhance our understanding of how cortical excitability impacts cognition. The local field potentials can provide templates of neural signatures that in future studies can be compared to electroencephalography data from humans with ASD or Fragile X.

项目概要自闭症谱系障碍 (ASD) 是一种神经发育障碍，个体在某些方面表现出缺陷认知灵活性。目的：本提案将从 Fmr1 KO 的额叶皮层进行记录（Fragile X 的模型）综合征）在认知灵活性任务期间并评估 KO 内的神经过度兴奋如何影响逆转学习和注意力转移。方法：Fmr1 KO 和野生型大鼠将接受分类训练在操作室中使用触摸屏执行感知任务。单单元神经生理学和局部场电位将在任务期间从额叶皮层（包括前扣带回）收集。重要的是了解 KO 内神经功能的改变如何影响认知功能和学习。该项目将比较假定的锥体神经元和中间神经元的神经活动，以检查期间的差异功能认识。检查 KO 和 WT 之间神经功能的这些差异将增强我们的研究能力。了解皮质兴奋性如何影响认知。局部场势可以提供模板在未来的研究中可以将神经特征与人类的脑电图数据进行比较 ASD 或脆性 X。