Functional Analytics Core

功能分析核心

• 批准号: 10643964
• 负责人: Johann Eli Gudjonsson
• 金额: $ 24.88万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-06-01 至 2024-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10643964
• 关键词: Acceleration; Autoimmune; Basic Science; Big Data; Biological; Biological databases; Biology; CRISPR/Cas technology; California; Cells; Communities; Community Outreach; Consultations; Cutaneous; Data; Data Analyses; Disease; Effectiveness; Epigenetic Process; Experimental Designs; Female; Gene Expression; Gene Expression Profiling; Generations; Genes; Genetic Code; Genetic Engineering; Genomics; Genotype-Tissue Expression Project; Goals; Human; Immune; Individual; Infrastructure; Knock-in; Knock-out; Knowledge; Los Angeles; Mediating; Michigan; Mission; Molecular; Mus; Mutagenesis; National Institute of Arthritis, and Musculoskeletal, and Skin Diseases; Pathologic; Phenotype; Publications; RNA; Reporting; Research; Research Infrastructure; Research Personnel; Resources; Services; Skin; System; Systems Biology; Techniques; Technology; Training; Training and Education; Transcript; Transfection; Translating; Translational Research; United States National Institutes of Health; Universities; bioinformatics resource; cell type; cytokine; data analysis pipeline; data integration; data sharing; design; differential expression; epigenomics; genetic manipulation; genome editing; genome wide association study; genome-wide; genomic data; high throughput screening; innovation; insight; keratinocyte; male; member; mutant; novel; repaired; skin disorder; statistics; tool; training opportunity; transcription factor; transcriptomics; web interface

PROJECT SUMMARY In recent years our abilities to manipulate the genetic code of individual cells, through CRISPR/Cas9 induced mutagenesis, and generation of high-throughput data have rapidly advanced. However, major bottlenecks remain; the use of CRISPR/Cas9 remains technically challenging, particularly in keratinocytes, which are much more challenging to transfect than other cell-types. Furthermore, the ability of most research groups to analyze high-throughput data and incorporate it into a meaningful biologic and pathologic context remains limited. Therefore, there is an enormous need for expertise and capabilities both for CRISPR/Cas9 services as well as bioinformatic resources to translate the vast information and connect it with relevant biological or disease-specific situation. To fill this critical gap, the University of Michigan Skin Biology and Resource-based Center (UM-SBDRC) Functional Analytics Core (FAC) will provide sophisticated infrastructure, resources, expertise, and training opportunities at the University of Michigan under the UM-SBDRC. The FAC is in line with the NIAMS mission to provide needed research infrastructure, pooled facilities, services, and resources to groups of investigators conducting research on skin biology and diseases. The goal of the FAC is to accelerate, enhance, and enrich the effectiveness of ongoing basic and translational research, and to help bring outside investigators into the field of cutaneous biology. This will be achieved through the two aims of the FAC: In Aim 1 where we provide CRISPR/Cas9 genome editing services in keratinocytes for generation of both knock-out and knock-in keratinocytes as well as functional characterization of mutant keratinocyte lines, and Aim 2, where we provide access to systems biology analyses, disease- and cytokine-specific gene expression profiling, and training. These services are not widely available for the cutaneous research community, and the services, resources, and techniques made available through this Core will provide UM- SBDRC Members with highly innovative approaches that will promote and accelerate ongoing research and help attract new research teams into the fold of cutaneous research.

项目摘要 近年来，我们通过CRISPR/CAS9引起的操纵单个细胞遗传密码的能力 诱变和高通量数据的产生迅速发展。但是，主要的瓶颈 保持; CRISPR/CAS9的使用在技术上仍然具有挑战性，尤其是在角质形成细胞中，这是很多 与其他细胞类型相比，转染更具挑战性。此外，大多数研究小组的能力 分析高通量数据并将其纳入有意义的生物学和病理环境中 有限的。因此，对CRISPR/CAS9服务的专业知识和能力都非常需要 以及生物信息学资源以转化大量信息，并将其与相关的生物学或 特定疾病的情况。为了填补密歇根大学皮肤生物学和基于资源的关键空白 中心（UM-SBDRC）功能分析核心（FAC）将提供复杂的基础架构，资源， 在UM-SBDRC下，密歇根大学的专业知识和培训机会。 FAC在排队 Niams的使命是提供所需的研究基础设施，合并设施，服务和资源 进行皮肤生物学和疾病研究的研究人员小组。 FAC的目标是 加速，增强和丰富正在进行的基础和转化研究的有效性，并帮助 将外部研究人员带入皮肤生物学领域。这将通过两个目标来实现 FAC：在AIM 1中，我们在角质形成细胞中提供CRISPR/CAS9基因组编辑服务以生成 敲除和敲除角质形成细胞以及突变角质形成细胞系的功能表征， 和AIM 2，我们可以在其中提供系统生物学分析，疾病和细胞因子特异性基因的访问权限 表达分析和训练。这些服务并不广泛用于皮肤研究 社区以及通过此核心提供的服务，资源和技术将提供 SBDRC成员采用高度创新的方法，将促进和加速正在进行的研究和 帮助吸引新的研究团队进入皮肤研究的范围。