Core C: Myeloid cell and atherosclerosis core

核心C：骨髓细胞和动脉粥样硬化核心

• 批准号: 10642743
• 负责人: Jenny E. Kanter
• 金额: $ 18.87万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10642743
• 关键词: Aorta; Arterial Fatty Streak; Arteries; Atherosclerosis; Bioinformatics; Biological Assay; Biology; Cardiovascular Diseases; Cell Compartmentation; Cells; Characteristics; Complement; Confocal Microscopy; Data; Data Set; Diabetes Mellitus; Digestion; Ensure; Family; Flow Cytometry; Gene Expression; Gene Expression Profiling; Histologic; Histology; Immunohistochemistry; Individual; Laboratories; Lesion; Lesion by Morphology; Lipoproteins; Macrophage; Measurement; Molecular; Morphology; Myeloid Cells; Procedures; Process; Program Research Project Grants; Protocols documentation; RNA analysis; Regulation; Reproducibility; Research; Research Personnel; Services; Site; Stains; Standardization; Techniques; Technology; Tissues; Triglycerides; Valsalva sinus; analytical method; analytical tool; cell type; cost effectiveness; histological stains; immunocytochemistry; instrumentation; laser capture microdissection; mouse model; novel; programs; single-cell RNA sequencing; tissue preparation; transcriptome sequencing

The Myeloid Cell and Atherosclerosis Core (Core C) within the Program Project “Triglycerides, Diabetes and Cardiovascular Disease” will provide individual projects with analytical techniques for thorough analysis of lesions of atherosclerosis from the different mouse models used within the program. These analyses will include histological staining methods, lesion size measurements, lesion morphology characterization, immunohistochemistry, and laser capture microdissection for analysis of gene expression. The core will also provide expertise and bioinformatics analysis of RNA-sequencing and single cell RNA-sequencing data as a complement to the Program Investigators’ projects. This Core’s repertoire of high-quality analyses of myeloid cells, atherosclerosis and other tissues studied within the Program Project will substantially enhance the scientific progress of the four individual projects.

“甘油三酯、糖尿病和糖尿病”计划项目中的骨髓细胞和动脉粥样硬化核心（核心 C） 心血管疾病”将为各个项目提供分析技术，以彻底分析 这些分析将来自该程序中使用的不同小鼠模型的动脉粥样硬化病变。 包括组织学染色方法、病变大小测量、病变形态特征、 免疫组织化学和激光捕获显微切割也将用于分析基因表达。 提供 RNA 测序和单细胞 RNA 测序数据的专业知识和生物信息学分析 该核心的高质量骨髓分析项目的补充。 该计划项目中研究的细胞、动脉粥样硬化和其他组织将大大增强 四个单项项目的科学进展。