Paper-based Breastmilk Collection System for Facile, In-Home Use

方便家庭使用的纸质母乳收集系统

• 批准号: 10641685
• 负责人: MARIA M. SANTORE
• 金额: $ 20.17万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10641685
• 关键词: 3-Dimensional; Acceleration; Address; Affect; Birth; Blood; Blood capillaries; Breast; Breast Cancer Risk Factor; Breast Epithelial Cells; Breast Feeding; Cancer Burden; Cancerous; Cells; Chemistry; Collecting Cell; Collection; DNA; DNA analysis; Detection; Development; Device Designs; Devices; Diagnosis; Diet; Disease; Dryness; Early Diagnosis; Early identification; Effectiveness; Eligibility Determination; Epigenetic Process; Epithelial Cells; Etiology; Event; Excision; Exposure to; Failure; Fiber; Freezing; Future; Geographic Locations; Home; Human; Human Milk; Ice; Individual; Knowledge; Liquid substance; Malignant Neoplasms; Mammary Gland Parenchyma; Mammary gland; Methods; Milk; Modeling; Molecular; Mutation; Nucleic Acids; Paper; Performance; Pharmaceutical Preparations; Phase; Population; Pregnancy; Primary Prevention; Privacy; Process; Property; Recommendation; Recovery; Refrigeration; Research Design; Research Personnel; Risk Assessment; Shapes; Shipping; Somatic Mutation; Specimen; Surface; System; Temperature; Testing; Time; Tissues; Woman; anticancer research; cancer risk; design; detection limit; disorder risk; epidemiology study; improved; innovation; insight; malignant breast neoplasm; methylation pattern; premalignant; prevent; programs; prototype; young woman

Project Summary / Abstract This project will conduct early-stage development of a paper-based device to facilitate the efficient recovery of high-quality nucleic acids from breastmilk for use in breast cancer risk assessment, detection of occult disease, and epidemiologic studies designed to uncover the earliest events in the development of breast cancer. Collecting breastmilk, which contains millions of epithelial cells from the mammary gland, is the only noninvasive method of obtaining breast tissue. Current breastmilk collection methods require expensive overnight shipping of breastmilk on ice and greatly limit studies using this important biospecimen. The proposed device will facilitate the collection and loading of breastmilk on a small paper card that can be sent through the mail and stored flat at room temperature for long periods of time, or indefinitely, allowing subsequent recovery of nucleic acids. Because specimens can be loaded by women in the privacy of their homes, and because the device is paper-based and inexpensive, we anticipate it will facilitate collection of nucleic acids from the breast cells of large numbers of women in diverse geographic locations. While the breastmilk-collection device is inspired by blood-collection cards, it is designed to process substantially larger volumes of breastmilk, needed to obtain a large quantity of nucleic acids required for cancer risk assessment. The device therefore will accomplish several functions: the manipulation of breastmilk into the paper through capillary action, the separation of excess fluid into a disposable layer and the retention of a nucleic-rich fraction within the primary device matrix. Removal of the excess fluid volume will expedite subsequent drying of the captured fluid and simplify transport in the event the user cannot thoroughly dry the card before transport. The device will additionally incorporate preservatives into the specimen to facilitate long term stability at room temperature. This program focuses on the design of the device to facilitate retention of nucleic acids and any cells (also containing nucleic acids) remaining unlysed in the processing window, at the same time removing excess fluid from this component of the device. As we target a paper-based matrix, the proposed studies will focus on evaluating the interaction of breastmilk with papers of varied pore size and surface chemistry with and without added preservatives. These studies will enable the prioritization of materials for different components of the device, a working configuration for the layers and other device features, and accelerated testing to gauge the impact of extreme transport and storage temperatures.

项目概要/摘要 该项目将进行纸质设备的早期开发，以促进高效 从母乳中回收高质量核酸用于乳腺癌风险评估、检测 隐匿性疾病和流行病学研究旨在揭示隐匿性疾病发展中的最早事件 乳腺癌。收集母乳，其中含有数百万来自乳腺的上皮细胞 腺，是获取乳腺组织的唯一非侵入性方法。目前的母乳收集方法 需要昂贵的隔夜运输来冰冻母乳，这极大地限制了使用这一重要母乳的研究 生物样本。所提出的装置将有助于在小纸上收集和装载母乳 可以通过邮件发送并在室温下长期平放的卡，或者 无限期地，允许随后回收核酸。因为标本可以由女性装载 在他们家中的隐私中，并且由于该设备是纸质的并且价格低廉，我们预计它将 促进从不同地理区域大量女性的乳腺细胞中收集核酸 地点。 虽然母乳采集设备的灵感来自于采血卡，但它的设计目的是处理 需要大量母乳才能获得所需的大量核酸 癌症风险评估。因此，该设备将完成多种功能： 母乳通过毛细作用进入纸中，将多余的液体分离成一次性层 以及富核级分保留在初级装置基质内。去除多余的液体 体积将加快捕获液体的后续干燥并简化运输（如果用户） 运输前不能彻底干燥卡片。该设备还将添加防腐剂 进入样品以促进室温下的长期稳定性。该计划重点关注 装置的设计有利于核酸和任何细胞（也含有核酸）的保留 在处理窗口中保持未裂解，同时从中去除多余的液体 设备的组成部分。由于我们的目标是基于纸质的矩阵，因此拟议的研究将集中于 评估母乳与不同孔径和表面化学的纸张的相互作用 不添加防腐剂。这些研究将使不同材料的优先顺序成为可能 设备的组件、各层的工作配置和其他设备功能，以及 加速测试以评估极端运输和储存温度的影响。