Novel Surfaces for Enriching Epithelial Cells: A Breastmilk Model

用于富集上皮细胞的新型表面：母乳模型

基本信息

批准号：
8435341
负责人：
MARIA M. SANTORE
金额：
$ 16.25万
依托单位：
UNIVERSITY OF MASSACHUSETTS AMHERST
依托单位国家：
美国
项目类别：
财政年份：
2012
资助国家：
美国
起止时间：
2012-03-01 至 2014-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8435341
关键词：
Accounting Address Adhesives Adsorption Antibodies Binding Body Fluids Breast Breast Cancer Early Detection Buffers Cancer cell line Cancerous Carcinoma Cell Adhesion Cell Fraction Cell Line Cell Separation Cell surface Cells Charge Childbirth Collecting Cell Collection DNA Detection Development Discrimination Early Diagnosis Early treatment Economic Development Elements Engineering Epithelial Epithelial Cells Exclusion Exploratory/Developmental Grant Fatty acid glycerol esters Flow Cytometry Foundations Future Harvest Human Human Milk Immunoglobulin Fragments Incidence Individual Lactation Liquid substance Literature Lymphocyte Malignant Neoplasms Malignant neoplasm of lung Measures Methods Methylation Microfluidic Microchips Microfluidics Milk Minor Modeling Modification Molecular Molecular Analysis Mucous body substance Pattern Performance Physiological Polymers Population Process Property Proteins Risk Risk Assessment Saliva Scheme Screening for cancer Specimen Surface Suspension substance Suspensions System Technology Testing Time Tissues Training Translating Urine Woman Work base biocompatible polymer cancer risk cancer therapy cancer type cell type combat design innovation instrumentation malignant breast neoplasm mortality nanoparticle nanoscale neuronal cell body novel programs research study scale up screening success

项目摘要

DESCRIPTION (provided by applicant): Harvesting and concentrating epithelial cells from body fluids is a challenging (expensive and time-consuming) but important task required for translational advances in the early-detection and treatment of cancer, since many cancer types involve epithelial cells. As an example, a highly promising and accurate risk detection method for breast cancer, based on changes in the DNA of breast epithelial cells found in human milk, requires purification and concentration of these cells, and exclusion of lymphocytes, fats, and proteins that are prevalent in milk. Because the necessary immediate epithelial enrichment process is costly in terms of labor, instrumentation, and lab supplies (antibody-coated beads), alternate methods of epithelial cell collection and concentration are needed. The proposed program will develop surfaces capable of selectively adhering epithelial cells from milk and rejecting the undesired cell fractions and molecules. The potential impact of this separation scheme comes from the innovative features of the surfaces themselves: robust and economical nano-scale adhesive elements such as polymer coils and immobilized nanoparticles are employed instead of expensive and fragile biomolecular fragments and antibodies. These sophisticated surfaces could be deployed in highly sensitive microfluidic devices or on beads, which could be used in the field by individuals who lack highly specialized training. The screening methods made possible by these innovative surfaces are expected to have wide accessibility and therefore broad positive impact for early detection of breast cancer. At the same time the surface design strategies developed in this program could, with minor modification, be applied to the creation of additional surfaces targeting cancer and pre-cancerous cell changes in other epithelial tissues, collected from urine, saliva, and mucus.

描述（由申请人提供）：从体液中收集和浓缩上皮细胞是一个具有挑战性的（昂贵且耗时），但要在癌症的早期检测和治疗中转化所需的重要任务，因为许多癌症类型涉及上皮细胞。例如，基于在人牛奶中发现的乳腺上皮细胞的DNA的变化，需要纯化和浓度这些细胞的乳腺癌的DNA，并且排除淋巴细胞，脂肪和蛋白质，这些乳腺癌的DNA的变化需要在牛奶中盛行。由于必要的直接上皮富集过程在劳动，仪器和实验室供应（抗体涂层珠）方面成本高昂，因此需要上皮细胞收集和浓度的替代方法。提出的程序将开发能够从牛奶中选择性粘附上皮细胞并拒绝不希望的细胞部分和分子的表面。这种分离方案的潜在影响来自表面本身的创新特征：强大而经济的纳米尺度粘合剂元素，例如聚合物线圈和固定的纳米颗粒，而不是昂贵且脆弱的生物分子碎片和抗体。这些复杂的表面可以部署在高度敏感的微流体设备或珠子中，这些设备可以由缺乏高度专业培训的个人在现场使用。这些创新的表面使筛查方法成为可能，预计将具有广泛的可及性，因此对乳腺癌的早期发现具有广泛的积极影响。同时，该程序中制定的表面设计策略可以通过较小的修饰来应用于靶向癌症和癌细胞前细胞变化的其他表面，并从尿液，唾液和粘液中收集的其他上皮组织变化。