Structure, Composition, & Histology Core - Core B

结构、组成、

• 批准号: 10459375
• 负责人: DAVID H. KOHN
• 金额: $ 25.22万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10459375
• 关键词: 3-Dimensional; Affect; Biological; Biological Assay; Blood Vessels; Cancer Grant Supplements (P30); Cartilage; Cells; Cellular biology; Chemistry; Clinic; Collaborations; Communication; Communities; Data; Data Analyses; Development; Disease; Education and Outreach; Engineering; Ensure; Experimental Designs; Fee-for-Service Plans; Future; Gene Expression; Genetic; Goals; Health; High Resolution Computed Tomography; Histologic; Histology; Image; Image Analysis; Interdisciplinary Study; Knowledge; Laboratories; Lead; Leadership; Length; Link; Measures; Mechanics; Michigan; Molecular; Morphology; Musculoskeletal; Needs Assessment; Organ; Outcome; Perfusion; Physiological; Process; Protocols documentation; Raman Spectrum Analysis; Recommendation; Recording of previous events; Reproducibility; Research; Research Personnel; Research Support; Resources; Science; Services; Standardization; Structure; Surveys; Technology; Testing; Tissues; Training; Training and Education; Translating; Universities; X-Ray Computed Tomography; base; biomedical imaging; bone; cost; functional outcomes; improved; innovation; instrument; microCT; nano; nanoscale; neural network; physical science; programs; ranpirnase; repository; response; tool; trait; treatment strategy; virtual

Michigan Integrative Musculoskeletal Health Core Center – Core B Project Summary Structure and composition across physiological length-scales are key intermediate traits that link gene expression with functional outcomes. Genetic and/or environmental perturbations affecting gene expression often lead to measureable changes in these intermediate traits that have mechanical and other functional consequences. Accurately quantifying structure and composition at different length scales is therefore essential to advancing our understanding of the molecular and mechanical mechanisms underlying musculoskeletal health and disease; to develop and evaluate treatment strategies; and to ultimately translate these strategies to the clinic. However, this research requires use of advanced and costly instruments and supporting expertise in cell biology, chemistry, engineering, and biomedical imaging. The Structure, Composition and Histology Core (Core B) will provide access to the instruments and expertise that will enable Center investigators to quantify these intermediate traits and to accelerate their research programs. Core B will include ex-vivo micro-Computed Tomography (µCT) and nano-Computed Tomography (nano-CT), Raman spectroscopy, and histology as its major components. These technologies were chosen for their applicability to all musculoskeletal tissues and based on usage data over the previous 4 years and a needs-assessment survey of core users. Investigators at the University of Michigan pioneered the use of µCT and Raman spectroscopy to investigate musculoskeletal tissues and are leaders in applying these technologies to solve musculoskeletal problems. Histology continues to be the gateway approach to understanding changes in tissue morphology and, when combined with CT and Raman spectroscopy, histology can link tissue morphology to composition and functional outcomes. The goals of this Core are to leverage the expertise of the core leadership to support the research programs of Center investigators in performing structural, compositional and histological analyses of all musculoskeletal tissues, both intellectually and financially; to expand opportunities for our research community; and to bring new investigators into the musculoskeletal community. To accomplish our goals and to continue creating a robust and sustainable impact to the musculoskeletal community in the P30 renewal application, we propose the following Specific Aims: Aim 1: To provide expertise and guidance on the best use of technologies and experimental design to allow investigators to conduct specialized assays for quantifying structure and composition across length scales. Aim 2: To provide expert use, standardization, and training in state-of-the-art technologies to quantify structure and composition across physiological length scales. Aim 3: To provide expertise and scientific guidance on data analysis, interpretation of results and recommendations for future studies. Aim 4: To expand opportunities for our research community through innovation and development of enabling technologies, education, training, and enrichment.

密歇根综合肌肉骨骼健康核心中心 – 核心 B 项目概要 跨生理长度尺度的结构和组成是连接基因的关键中间特征 影响基因表达的遗传和/或环境扰动。 通常会导致这些具有机械和其他功能的中间特征发生可测量的变化 因此，准确量化不同长度尺度的结构和组成至关重要。 增进我们对肌肉骨骼健康的分子和机械机制的理解 和疾病；制定和评估治疗策略；并最终将这些策略转化为 然而，这项研究需要使用先进且昂贵的仪器以及支持细胞专业知识。 生物学、化学、工程和生物医学成像核心。 （核心 B）将提供工具和专业知识，使中心研究人员能够量化 这些中间特征和加速他们的研究计划的核心B将包括体外微计算。 断层扫描 (μCT) 和纳米计算机断层扫描 (nano-CT)、拉曼光谱和组织学作为其 选择这些技术是因为它们适用于所有肌肉骨骼组织和 基于过去 4 年的使用数据以及对核心用户的需求评估调查。 密歇根大学率先使用 µCT 和拉曼光谱来研究肌肉骨骼 组织，并且是应用这些技术解决肌肉骨骼问题的领导者。 与 CT 和 CT 相结合，成为了解组织形态变化的门户方法 拉曼光谱、组织学可以将组织形态与成分和功能结果联系起来。 该核心的目的是利用核心领导层的专业知识来支持中心的研究项目 研究人员对所有肌肉骨骼组织进行结构、成分和组织学分析 智力和经济上；扩大我们研究界的机会；并引进新的研究人员； 进入肌肉骨骼界，以实现我们的目标并继续创建一个强大且可持续的社区。 为了在 P30 更新申请中对肌肉骨骼社区的影响，我们提出以下具体目标： 目标 1：提供有关技术最佳使用和实验设计的专业知识和指导，以允许 研究人员进行专门的分析，以量化跨长度尺度的结构和成分。 目标 2：提供最先进技术的专家使用、标准化和培训，以量化结构 和跨生理长度尺度的组成。 目标 3：提供有关数据分析、结果解释和分析的专业知识和科学指导 对未来研究的建议。 目标 4：通过创新和发展赋能技术，为我们的研究界扩大机会 技术、教育、培训和丰富。