Milk Analytics Core

牛奶分析核心

• 批准号: 10309713
• 负责人: Lars Bode
• 金额: $ 20.51万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10309713
• 关键词: Aliquot; Analytical Chemistry; Antibiotic Therapy; Antibiotics; Biological; Biological Assay; Biomedical Research; Breast Feeding; Breastfed infant; California; Cells; Childhood; Clinical; Clinical Data; Clinical Sciences; Collaborations; Collection; Communities; Complex; Coupled; Data; Data Science; Detection; Fluorescence; Goals; Growth Factor; High Pressure Liquid Chromatography; Hormones; Human Characteristics; Human Milk; Immunoassay; Infant; Infant Development; Infant Health; Infant Mortality; Knowledge; Lactation; Lactose; Leukocyte L1 Antigen Complex; Lipids; Macronutrients Nutrition; Maternal and Child Health; Measurement; Measures; Microbe; Micronutrients; Milk; Mothers; Mus; Mutation; Near-Infrared Spectroscopy; Oligosaccharides; Outcome; Participant; Peptide Hydrolases; Peptides; Performance; Pregnancy; Proteins; Protocols documentation; Research; Research Personnel; Research Project Grants; Resources; Risk; Role; Safety; Sampling; Services; Ships; Technology; Therapeutic; Therapeutic Agents; Therapeutic Human Experimentation; Triad Acrylic Resin; Universities; Validation; Woman; analytical tool; assay development; biological heterogeneity; cytokine; design; experience; feeding; ghrelin; glucagon-like peptide 1; improved; infant morbidity; lipidomics; macromolecule; metabolomics; microbiome; microbiome analysis; milk fat globule; milk microbiome; operation; pharmacometrics; programs; response; sample collection; skills

ABSTRACT The University of California San Diego (UC San Diego) MPRINT Center of Excellence in Therapeutics (CET) involves a human milk (HM)-centered approach to investigate the role of maternal and pediatric therapeutics at intersections of the mother-milk-infant ‘triad’. Key among the overall goals of the MPRINT CET are to assess how maternal antibiotics impact HM composition and how HM components alter safety and efficacy of these important pediatric therapeutic agents. HM composition analysis, however, is not trivial. HM composition is highly dynamic and changes throughout lactation, indeed even throughout the course of a single feeding. HM is a complex matrix that includes cells and microbes as well as large molecule aggregates like the milk fat globule. HM also contains a unique set of molecules, the human milk oligosaccharides (HMOs), which represent the third most abundant component of HM after lactose and lipids. Thus, the dynamic, complex, and unique nature of HM requires specific collection protocols, assay development, and assay validation. With over 20 years of experience in HM research, the Milk Analytics Core (MAC) will provide overall guidance and advice to MPRINT CET investigators and staff. MAC will provide HM collection protocols and ready-to-ship sample collection kits to the Clinical and Data Science Projects (Aim 1a) and receive HM samples for composition analysis (Aim 1b). Depending on project needs, MAC offers macronutrient, oligosaccharide, and bioactive measurements as well as milk microbiome analysis, the latter in collaboration with the UC San Diego Microbiome Core, which operates independently of, but frequently in partnership with, our team. MAC will also provide mouse milk oligosaccharide and microbiome analyses to the Research Project team (Aim 1c), applying our detailed prior knowledge of how the St3gal4 mutation under study markedly depletes sialylated milk oligosaccharide content. Data generated from our analyses will be returned to the respective project teams for integration. Separate HM aliquots from the same samples will be passed directly on to the Pharmacometrics and Analytical Chemistry Core for antibiotic drug quantification, and that data will likewise be directly returned to the respective project teams. In addition to already established services, MAC will develop and validate new HM-specific assays by expanding the analyte portfolio of the existing multiplex immunoassay platform (Aim 2a) and by engaging and activating other UC San Diego researchers to apply and validate their unique technologies to HM research (Aim 2b). We envision MAC to become a comprehensive milk analytics core with knowledge, skills, and technology, serving as a resource to this and other MPRINT CETs and the biomedical research community at large. Together, we will be able to generate a better understanding how antibiotic treatment during pregnancy and lactation impacts the mother- milk-infant ‘triad’, inform and adjust precision in therapeutics, and ultimately improve maternal-child health.

抽象的 加利福尼亚大学圣地亚哥分校（加州大学圣地亚哥分校）MPRINT卓越治疗中心（CET） 涉及以人牛奶（HM）为中心的方法来研究母亲和小儿疗法在 母亲 - 米尔克的“三合会”的交叉点。 MPRINT CET的总体目标中关键是评估 产妇抗生素如何影响HM组成以及HM成分如何改变这些的安全性和效率 重要的儿科治疗剂。但是，HM组成分析并不是微不足道的。 HM组成很高 在整个哺乳过程中，动态和变化，甚至在整个喂养过程中甚至在整个喂养过程中。 hm是一个 包括细胞和微生物以及大分子聚集体（如牛奶脂肪球）的复杂基质。 HM还包含一组独特的分子，即人乳寡糖（HMOS），代表第三个 乳糖和脂质后HM的最绝对成分。那是HM的动态，复杂和独特的本质 需要特定的收集协议，测定开发和测定验证。拥有20多年的经验 在HM研究中，牛奶分析核心（MAC）将为MPRINT CET提供总体指导和建议 调查人员和工作人员。 MAC将为HM收集协议和现成的样本收集套件 临床和数据科学项目（AIM 1A）并接收HM样品进行组成分析（AIM 1B）。 根据项目需求，MAC还提供大量营养素，寡糖和生物活性测量 作为牛奶微生物组分析，后者与加州大学圣地亚哥分校的微生物核心合作，该核心运行 独立于我们的团队，但经常与我们的团队合作。 MAC还将提供牛奶寡糖 并向研究项目团队（AIM 1C）分析微生物组，应用我们详细的先验知识 研究中的ST3GAL4突变显着耗尽了溶解的牛奶寡糖含量。生成的数据 从我们的分析中将返回各自的项目团队进行集成。分开的HM等分试样与 相同的样品将直接传递到抗生素的药物计量学和分析化学核心 药物量化，这些数据同样将直接返回到各自的项目团队。此外 Mac已经建立的服务将通过扩展分析物来开发和验证新的HM特定测定法 现有多重免疫测定平台（AIM 2A）的投资组合，并通过参与和激活其他UC SAN 迭戈研究人员将其独特技术应用于HM研究（AIM 2B）。我们设想Mac 成为具有知识，技能和技术的综合牛奶分析核心，作为一种资源 这个和其他MPRINT CET以及整个生物医学研究界。在一起，我们将能够 可以更好地了解怀孕期间抗生素治疗和泌乳如何影响母亲 牛奶的“三合会”，告知和调整治疗的精度，并最终改善产妇的健康。