Live Cell Fluorescent Imaging System

活细胞荧光成像系统

• 批准号: 10424798
• 负责人: Glen Marrs
• 金额: $ 24.99万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-01 至 2023-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10424798
• 关键词: Area; Biochemical; Biology; Cells; Core Facility; Data; Disease; Equipment; Fluorescence; Goals; Health; Human; Image; Investigation; Measures; Microbe; Microscopic; Microscopy; Molecular; Morphology; Organism; Research; Research Personnel; Sampling; Speed; System; Time; Tissue imaging; Universities; Visualization; fluorescence imaging; forest; imager; imaging system; meetings; microbial; microscopic imaging; novel; novel strategies; tool

PROJECT SUMMARY/ABSTRACT A developing area of focus for Wake Forest University is the investigation of cellular and molecular aspects of microbial organisms. Novel research goals being pursued by these study teams will uncover detailed biochemical mechanisms within microbes that have direct relevance to human health and disease states. Microscopy represents a critical tool for all the studies outlined here with investigators collectively proposing emphasis on morphologic data measures that require acquisition of exceptional quality fluorescent and brightfield images. While the Wake Forest Microscopic Imaging Core Facility has been an historic area of strength for the Department of Biology, current needs are exceeding the available equipment capabilities in terms of both specific functionality and available imaging time. The Leica Thunder Live Cell Imager system requested here represents an ideal platform for meeting the directed research goals and plans outlined in this proposal. It will expand our existing capabilities by providing a high-speed fluorescence acquisition system with optimized living tissue imaging conditions, and will provide a new approach for automated large sample territory visualization and detailed image acquisition. In addition to providing equipment that will be crucial to carry out the directed activities outlined here, this system will serve as a key addition to broaden our extant technical framework that stimulates and supports microscopic investigations of biology and human health in our region.

项目摘要/摘要 韦克森林大学的重点发展领域是对细胞和分子的研究 微生物的各个方面。这些研究团队正在追求的新颖研究目标将 发现与人类健康有直接相关的微生物中详细的生化机制 和疾病状态。显微镜代表了此处概述的所有研究的关键工具 研究人员集体提出了需要获取的形态数据指标的重视 优质荧光和明亮的图像。而韦克森林微观成像 核心设施一直是生物学系的历史性领域，当前的需求是 就特定功能和可用的可用设备功能超出了可用的设备功能 成像时间。这里请求的Leica Thunder Live Cell Imager系统代表一个理想 实现该提案中概述的有指导研究目标和计划的平台。它将扩大我们的 通过提供优化生活的高速荧光采集系统，通过提供现有功能 组织成像条件，并将为自动化大型样品区域提供一种新的方法 可视化和详细的图像获取。除了提供对设备至关重要的设备 进行此处概述的定向活动，该系统将是扩大我们的关键 现有的技术框架刺激并支持生物学的微观研究和 我们地区的人类健康。