TRD1: Interventional Fluorescence Lifetime Imaging Microscopy (iFLIM)

TRD1：介入荧光寿命成像显微镜 (iFLIM)

• 批准号: 10424947
• 负责人: Laura Marcu
• 金额: $ 23.46万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-20 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10424947
• 关键词: Address; Adoption; Architecture; Biochemical; Biochemistry; Biophotonics; Biopsy; Brain; Breast; Calibration; Cardiovascular system; Catheters; Cell Culture Techniques; Classification; Clinical; Collection; Complex; Coupling; Data; Data Aggregation; Data Analyses; Databases; Decision Making; Detection; Development; Devices; Diagnosis; Diagnostic; Electronics; Engineering; Evaluation; Excision; Fiber; Fiber Optics; Fluorescence; Head and neck structure; Imaging Device; Imaging technology; In Situ; Intervention; Knowledge; Label; Light; Link; Measurement; Mechanics; Metabolic; Methodology; Microscopy; Modality; Molecular; Molecular Probes; Morphology; Multi-Institutional Clinical Trial; Multimodal Imaging; Normal tissue morphology; Operative Surgical Procedures; Optics; Organ; Pathologic; Pattern; Performance; Procedures; Property; Prostate; Protocols documentation; Site; Spectrum Analysis; Speed; Standardization; Surgeon; Surgical Oncology; System; Techniques; Technology; Time; Tissue Transplantation; Tissues; Translating; advanced analytics; analytical method; animal imaging; base; complex data; cost effective; data acquisition; data streams; deep learning; design; detector; fluorescence lifetime imaging; imaging modality; improved; innovation; instrumentation; lens; microendoscopy; miniaturize; minimally invasive; nervous system disorder; optical imaging; photonics; prototype; quantitative imaging; synergism; technology research and development; tumor

PROJECT SUMMARY – Technology Research and Development Project #1 The objective of TRD 1 is to technologically advance and integrate interventional fluorescence lifetime imaging (iFLIM) technology in clinical settings for real-time in-situ tissue diagnosis and guidance of surgical procedures. While the basic principles of FLIM technology are well-established and FLIM-based techniques are used extensively in cell culture microscopy and small-animal imaging, FLIM’s complex instrumentation and engineering difficulties for compact clinical fiber probes, long data acquisition times, and complex data analysis present significant barriers to its wide dissemination and limits its clinical adoption. TRD 1 joins the unique expertise in iFLIM at UC Davis with expertise in detector technologies, photonic integration, micro-endoscopy, and optical imaging to address current technological barriers, advance fundamental FLIM technology, and achieve greater clinically utility. TRD 1 consists of four Specific Aims: Aim 1 will improve iFLIM performance through the advancement of hyperspectral detector array built with monolithically integrated encoder patterns. New iFLIM detector technology with significantly improved sensitivity (10-fold) and speed (5-to-10-fold enhancement) compared to current technology and ability to perform fast multispectral measurements will be created. Aim 2 will expand iFLIM clinical utility by building devices capable of real-time, highly specific, quantitative imaging. Scalable miniaturized iFLIM systems that integrate planar imaging devices (spectrometer- on-a-chip) with high-speed readout integrated electronics (ROIC) based on innovative packaging strategies will be generated. Aim 3 will link iFLIM with other optical imaging devices. We will develop interfacing components based on advanced fiber optic and micro-endoscopic technology to enable multimodal imaging and access to internal organs. iFLIM compatible catheter technologies enabling coupling of iFLIM with other optical modalities (e.g., OCT, iDOS) permit simultaneous evaluation of complementary tissue signatures and expansion of iFLIM indications from open-field surgery to stereotactic biopsy, endovascular techniques and other minimally invasive interventions. Aim 4 will incorporate iFLIM into surgical guidance. We will develop strategies to integrate iFLIM into procedural workflow. Methodologies for standardization and effective integration of iFLIM devices in a clinically viable workflow will be generated. Impact: This TRD will create innovative, scalable iFLIM technology for intraprocedural use and generate prototypes and intraprocedural methodologies/protocols enabling wide dissemination of iFLIM devices, including through CPs and SPs, to promote their broader adoption. The acquired FLIm parameter database/cluster will enable subsequent multi-center clinical trials for automated tissue classification and diagnostic prediction. Although the initial clinical focus of this TRD is surgical oncology, advanced iFLIM should prove useful in other procedures (e.g., cardiovascular, neurologic disease, organ/tissue transplantation).

项目摘要 – 技术研发项目#1 TRD 1 的目标是技术进步和集成介入荧光寿命成像 (iFLIM) 技术在临床环境中用于实时原位组织诊断和外科手术指导。 虽然 FLIM 技术的基本原理已经很成熟，并且基于 FLIM 的技术也得到了使用 主要用于细胞培养显微镜和小动物成像，FLIM 的复杂仪器和 紧凑型临床光纤探头的工程困难、数据采集时间长和数据分析复杂 TRD 1 的广泛传播存在重大障碍，并限制了其临床应用。 加州大学戴维斯分校 iFLIM 专业知识，在探测器技术、光子集成、显微内窥镜、 和光学成像，以解决当前的技术障碍，推进基础 FLIM 技术，以及 TRD 1 包含四个具体目标： 目标 1 将提高 iFLIM 性能。 通过采用单片集成编码器模式构建的高光谱探测器阵列的进步。 新的 iFLIM 检测器技术显着提高了灵敏度（10 倍）和速度（5 至 10 倍） 增强）与当前技术相比，执行快速多光谱测量的能力将 Aim 2 将通过构建能够实时、高度特异性、 集成定量平面成像设备（光谱仪）的可扩展小型化 iFLIM 系统。 片上）与基于创新封装策略的高速读出集成电子器件（ROIC） Aim 3 将把 iFLIM 与其他光学成像设备连接起来。 基于先进的光纤和显微内窥镜技术，可实现多模态成像并获取 内脏器官兼容 iFLIM 导管技术，可将 iFLIM 与其他光学方式耦合。 （例如 OCT、iDOS）允许同时评估互补组织特征和 iFLIM 扩展 适应症从开放视野手术到立体定向活检、血管内技术和其他微创技术 目标 4 将 iFLIM 纳入手术指导 我们将制定整合 iFLIM 的策略。 将 iFLIM 设备标准化和有效集成到程序工作流程中。 将产生临床上可行的工作流程：该 TRD 将创建创新的、可扩展的 iFLIM 技术。 用于程序内使用并生成原型和程序内方法/协议，从而实现广泛的 传播 iFLIM 设备，包括通过 CP 和 SP，以促进其更广泛的采用。 FLIm参数数据库/集群将使后续的自动化组织多中心临床试验成为可能 尽管该 TRD 最初的临床重点是外科肿瘤学， 先进的 iFLIM 应该被证明在其他手术中有用（例如心血管、神经系统疾病、器官/组织） 移植）。