TRD1: Interventional Fluorescence Lifetime Imaging Microscopy (iFLIM)

TRD1：介入荧光寿命成像显微镜 (iFLIM)

• 批准号: 10424947
• 负责人: Laura Marcu
• 金额: $ 23.46万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-20 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10424947
• 关键词: Address; Adoption; Architecture; Biochemical; Biochemistry; Biophotonics; Biopsy; Brain; Breast; Calibration; Cardiovascular system; Catheters; Cell Culture Techniques; Classification; Clinical; Collection; Complex; Coupling; Data; Data Aggregation; Data Analyses; Databases; Decision Making; Detection; Development; Devices; Diagnosis; Diagnostic; Electronics; Engineering; Evaluation; Excision; Fiber; Fiber Optics; Fluorescence; Head and neck structure; Imaging Device; Imaging technology; In Situ; Intervention; Knowledge; Label; Light; Link; Measurement; Mechanics; Metabolic; Methodology; Microscopy; Modality; Molecular; Molecular Probes; Morphology; Multi-Institutional Clinical Trial; Multimodal Imaging; Normal tissue morphology; Operative Surgical Procedures; Optics; Organ; Pathologic; Pattern; Performance; Procedures; Property; Prostate; Protocols documentation; Site; Spectrum Analysis; Speed; Standardization; Surgeon; Surgical Oncology; System; Techniques; Technology; Time; Tissue Transplantation; Tissues; Translating; advanced analytics; analytical method; animal imaging; base; complex data; cost effective; data acquisition; data streams; deep learning; design; detector; fluorescence lifetime imaging; imaging modality; improved; innovation; instrumentation; lens; microendoscopy; miniaturize; minimally invasive; nervous system disorder; optical imaging; photonics; prototype; quantitative imaging; synergism; technology research and development; tumor

PROJECT SUMMARY – Technology Research and Development Project #1 The objective of TRD 1 is to technologically advance and integrate interventional fluorescence lifetime imaging (iFLIM) technology in clinical settings for real-time in-situ tissue diagnosis and guidance of surgical procedures. While the basic principles of FLIM technology are well-established and FLIM-based techniques are used extensively in cell culture microscopy and small-animal imaging, FLIM’s complex instrumentation and engineering difficulties for compact clinical fiber probes, long data acquisition times, and complex data analysis present significant barriers to its wide dissemination and limits its clinical adoption. TRD 1 joins the unique expertise in iFLIM at UC Davis with expertise in detector technologies, photonic integration, micro-endoscopy, and optical imaging to address current technological barriers, advance fundamental FLIM technology, and achieve greater clinically utility. TRD 1 consists of four Specific Aims: Aim 1 will improve iFLIM performance through the advancement of hyperspectral detector array built with monolithically integrated encoder patterns. New iFLIM detector technology with significantly improved sensitivity (10-fold) and speed (5-to-10-fold enhancement) compared to current technology and ability to perform fast multispectral measurements will be created. Aim 2 will expand iFLIM clinical utility by building devices capable of real-time, highly specific, quantitative imaging. Scalable miniaturized iFLIM systems that integrate planar imaging devices (spectrometer- on-a-chip) with high-speed readout integrated electronics (ROIC) based on innovative packaging strategies will be generated. Aim 3 will link iFLIM with other optical imaging devices. We will develop interfacing components based on advanced fiber optic and micro-endoscopic technology to enable multimodal imaging and access to internal organs. iFLIM compatible catheter technologies enabling coupling of iFLIM with other optical modalities (e.g., OCT, iDOS) permit simultaneous evaluation of complementary tissue signatures and expansion of iFLIM indications from open-field surgery to stereotactic biopsy, endovascular techniques and other minimally invasive interventions. Aim 4 will incorporate iFLIM into surgical guidance. We will develop strategies to integrate iFLIM into procedural workflow. Methodologies for standardization and effective integration of iFLIM devices in a clinically viable workflow will be generated. Impact: This TRD will create innovative, scalable iFLIM technology for intraprocedural use and generate prototypes and intraprocedural methodologies/protocols enabling wide dissemination of iFLIM devices, including through CPs and SPs, to promote their broader adoption. The acquired FLIm parameter database/cluster will enable subsequent multi-center clinical trials for automated tissue classification and diagnostic prediction. Although the initial clinical focus of this TRD is surgical oncology, advanced iFLIM should prove useful in other procedures (e.g., cardiovascular, neurologic disease, organ/tissue transplantation).

项目摘要 - 技术研发项目＃1 TRD 1的目的是在技术上进步和整合介入的荧光寿命成像 （IFLIM）在临床环境中进行实时原位组织诊断和手术程序指导的技术。 虽然Flim技术的基本原理是完善的，并且使用了基于FLIM的技术 在细胞培养显微镜和小动物成像中广泛，Flim的复杂仪器和 紧凑型临床纤维探针，较长的数据获取时间和复杂数据分析的工程难度 对其广泛传播并限制了其临床采用的巨大障碍。 TRD 1加入独特 在UC Davis的IFLIM方面具有专业知识，具有探测器技术专业知识，光子整合，微观镜检查， 和光学成像，以应对当前的技术障碍，提前的基本脆弱技术和 实现更大的临床用途。 TRD 1由四个特定目标组成：AIM 1如果Lim绩效，将改善 通过通过单层整合编码器模式构建的高光谱检测器阵列的发展。 新的iflim检测器技术具有显着提高的灵敏度（10倍）和速度（5至10倍） 与当前的技术和执行快速多光谱测量的能力相比，增强功能将是 创建。 AIM 2将通过构建能够实时，高度特定的设备来扩展iflim临床公用事业 定量成像。可扩展的小型IFLIM系统，集成了平面成像设备（光谱仪 - 基于创新包装策略的高速读数集成电子设备（ROIC），将 被生成。 AIM 3将与其他光学成像设备联系起来。我们将开发接口组件 基于高级光纤和微观镜面技术，以实现多模式成像并访问 内脏。 iflim兼容的成型技术，可以与其他光学方式耦合 （例如，ICT，IDO）允许同时评估完整的组织特征和Iflim的扩展 从开场手术到立体定向活检，血管内技术和其他微创的适应症 干预措施。 AIM 4将使IFLIM纳入外科指导。我们将制定策略以整合iflim 进入程序工作流程。标准化和有效整合IFLIM设备的方法论 将生成临床上可行的工作流程。影响：Iflim技术将创建创新的，可扩展的TRD 用于经术内的使用和生成原型和手术室内方法/协议，使得能够宽 传播IFLIM设备，包括通过CPS和SPS，以促进其更广泛的采用。获得的 FLIM参数数据库/群集将对自动化组织进行随后的多中心临床试验 分类和诊断预测。尽管该TRD的最初临床重点是外科肿瘤学，但 先进的iflim应该在其他程序中有用（例如心血管，神经疾病，器官/组织 移植）。