Training in isotopic techniques for metabolic research

代谢研究同位素技术培训

• 批准号: 10229467
• 负责人: OWEN P MCGUINNESS
• 金额: $ 10.45万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-13 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10229467
• 关键词: Animal Model; Animals; Area; Biomedical Research; Carbohydrates; Cells; Cellular biology; Communities; Computer software; Country; Data; Development; Diabetes Mellitus; Diabetic mouse; Discipline; Disease; Doctor of Philosophy; Educational Activities; Educational Curriculum; Equilibrium; Experimental Designs; Faculty; Fatty acid glycerol esters; Feedback; Funding; Funding Opportunities; Genetic; Glucose Clamp; Home; Human; In Vitro; Incidence; Institution; International; Isotopes; Kinetics; Knowledge; Mass Spectrum Analysis; Measurement; Mentors; Metabolic; Metabolic Diseases; Metabolic Pathway; Metabolism; Methodology; Methods; Mission; Modeling; Modeling of Cellular Pathways; Molecular Biology; Monitor; Mus; National Institute of Diabetes and Digestive and Kidney Diseases; Nuts; Obesity; Organ; Participant; Pathway interactions; Phenotype; Physicians; Procedures; Proteins; Protocols documentation; Radioisotopes; Regulation; Research; Research Personnel; Research Project Grants; Sampling; Science; Scientist; Services; Students; Techniques; Technology; Time; Tracer; Training; Training Programs; United States National Institutes of Health; Universities; Wood material; amino acid metabolism; base; design; energy balance; experience; in vivo; lectures; medical schools; metabolic abnormality assessment; metabolic phenotype; metabolomics; next generation; nutrition; oxidation; programs; protein metabolism; response; skill acquisition; skills; stable isotope; theories; tool; web site; webinar

Project Summary This application is in response to Funding Opportunity Announcement PAR-15-139 a five day course entitled” Isotope Tracers in Metabolic Research: Principles and Practice in Kinetic Analysis”. The course faculty will present state of the art methods for using both radioactive and stable isotopes to investigate whole body and organ metabolism in vivo and intracellular flux rates and pathway regulation in vivo and in vitro. The basic aspects of modeling will be considered, as well as specific applications to the study of carbohydrate, fat, protein metabolism and energy balance. Theoretical and practical matters related to sample analysis by mass spectrometry and NMR will be discussed, including detailed numerical examples of calculations involved in determining isotopic enrichment and basic kinetic parameters. Advanced lectures will discuss in more detail the use of positional and mass isotopomer analysis for intracellular flux rates and various aspects of protein and amino acid metabolism. Specific applications (hyperinsulinemic euglycemic clamp) for use in humans and animals will be presented. The course uses a lecture format combined with problem discussions. In addition a popular aspect of the course is that trainees meet one on one with course faculty to discuss their specific research project and can present their project in an evening session. A new addition is detailed theory and experimental protocols to monitor specific pathways in a webinar style format that will be available on the course website to the entire research community. Typically, 75-85 trainees attended the course each year. Feedback from attendees has been very laudatory. A number of trainees have developed new research projects using isotope technologies. Thus this popular course builds on and reinforces fundamental skills needed to study metabolic processes that are relevant to the mission of NIDDK.

项目概要 此申请是为了响应资助机会公告 PAR-15-139，为期五天 课程题为“代谢研究中的同位素示踪剂：动力学原理与实践” 分析”课程的教师将介绍使用放射性和放射性物质的最先进的方法。 稳定同位素用于研究全身和器官的体内代谢和细胞内通量 体内和体外的速率和途径调节是建模的基本方面。 考虑以及在碳水化合物、脂肪、蛋白质代谢研究中的具体应用 与质量样品分析相关的理论和实践问题。 将讨论光谱测定法和核磁共振，包括详细的计算数值示例 参与确定同位素富集和基本动力学参数。 将更详细地讨论位置和质量同位素分析在细胞内的应用 通量率以及蛋白质和氨基酸代谢的各个方面的具体应用。 将介绍用于人类和动物的（高胰岛素正常血糖钳夹）。 课程采用讲座形式并结合问题讨论。 该课程的重点是学员与课程教师进行一对一的会面，讨论他们的具体情况 研究项目，并可以在晚间会议上展示他们的项目 详细介绍了新的补充内容。 以网络研讨会形式监测特定途径的理论和实验方案 将在课程网站上向整个研究界公开，通常为 75-85。 每年参加该课程的学员的反馈都非常好。 许多学员利用同位素技术开发了新的研究项目。 这门受欢迎的课程建立在并加强了研究代谢所需的基本技能 与 NIDDK 使命相关的流程。