Mouse Metabolic Physiology Core

小鼠代谢生理学核心

基本信息

批准号：
10588962
负责人：
OWEN P MCGUINNESS
金额：
$ 49.75万
依托单位：
VANDERBILT UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
2023
资助国家：
美国
起止时间：
2023-02-01 至 2028-01-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10588962
关键词：
Abbreviations Address Animals Behavioral Behavioral Mechanisms Blood Vessels Body Weight Brain Brain region Cannulas Cannulations Carbon Dioxide Catheterization Catheters Communities Complex Conscious Consultations Dedications Development Diabetes Mellitus Education Energy Metabolism Ensure Exercise Experimental Designs Facility Designs Faculty Feeding behaviors Fiber Optics Floor Genetic Genetic Models Glucose Clamp Hormones Housing Human Resources Implant Indwelling Catheter Institution Insulin Investments Islets of Langerhans Transplantation Light Measurement Measures Metabolic Metabolic Diseases Metabolism Modeling Mus Obesity Operative Surgical Procedures Output Oxygen Participant Physiological Physiology Population Heterogeneity Procedures Recording of previous events Regulation Research Research Personnel Resources Rewards Role Scientist Services Standardization Stress Surgeon Techniques Technology Time Tissue Transplantation Training Underrepresented Populations Venous Viral Work animal care anterior chamber bariatric surgery career design energy balance exercise capacity experience experimental study eye chamber flexibility gene environment interaction human disease implantation in vivo innovation inter-institutional metabolic abnormality assessment metabolic phenotype motivated behavior mouse genetics mouse model neuroregulation novel nutrient metabolism optical fiber outreach programs sample collection skills surgical service tool uptake

项目摘要

Project Summary The Mouse Metabolic Physiology Core and its two subcores, the Metabolic Regulation Subcore and the Body Weight Regulation Subcore, provide investigators both guidance on experimental design and use of state-of- the-art techniques to assess genetic and environmental determinants of insulin action, substrate metabolism, and energy balance in vivo. The experienced faculty and highly skilled staff of this Core have a 20-year history of performing complex procedures to study metabolism in healthy, unstressed mice and have been at the forefront of development, standardization, implementation, and dissemination of new concepts and techniques to study mouse models of metabolic diseases. In the present application, the Mouse Metabolic Physiology Core delivers critically needed mouse-related services to investigators outside of Vanderbilt. Skilled mouse surgeons perform difficult surgeries such as catheter, brain cannula, and brain probe implantations, as well as bariatric surgery and islet transplantation. Experienced staff perform complex experiments such as metabolic flux analyses under conditions such as those created by a glucose clamp or exercise. Measurements of energy balance components and assessment of reward/motivated behavior allow for the physiological and behavioral determinants of body weight to be determined in mouse models of metabolic disease. This Core merges techniques so that oxygen uptake, carbon dioxide output, energy expenditure, activity and feeding behavior are measured simultaneously with studies of metabolism using indwelling catheters or neural regulation using implanted cannula and fiber optic probes. Experience at Vanderbilt over the past 5 years predicts robust use of this Core. The Mouse Metabolic Physiology Core participates in important educational programs for the diabetes community, including a weeklong course that has been given 20 times over 19 years focusing on surgical and experimental techniques necessary to perform glucose clamps in mice. This Core supports the Vanderbilt Vibrant Program by providing necessary time and resources to investigators from underrepresented populations or institutions and early-career investigators. In summary, the Core facilitates diabetes, obesity, and metabolism research by providing novel services that are feasible at few other institutions to investigators outside of Vanderbilt.

项目概要小鼠代谢生理学核心及其两个子核心：代谢调节子核心和身体体重调节子核心，为研究人员提供实验设计和使用状态的指导最先进的技术来评估胰岛素作用、底物代谢的遗传和环境决定因素，以及体内的能量平衡。该核心的经验丰富的教师和高技能的员工拥有 20 年的历史执行复杂的程序来研究健康、无压力的小鼠的新陈代谢，并且一直处于新概念和技术的开发、标准化、实施和传播的前沿研究代谢疾病的小鼠模型。在本申请中，小鼠代谢生理学 Core 为范德比尔特以外的研究人员提供急需的鼠标相关服务。熟练的鼠标外科医生进行导管、脑插管和脑探针植入等困难的手术，以及减肥手术和胰岛移植。经验丰富的工作人员进行复杂的实验，例如代谢在葡萄糖钳夹或运动等条件下进行通量分析。能量测量平衡成分和奖励/动机行为的评估允许生理和行为在代谢疾病小鼠模型中确定体重的决定因素。这个核心合并了技术，使摄氧量、二氧化碳输出量、能量消耗、活动和摄食行为与使用留置导管的代谢研究或使用神经调节的研究同时进行测量植入套管和光纤探头。范德堡大学过去 5 年的经验表明，这个核心。小鼠代谢生理学核心参与重要的教育项目糖尿病社区，包括为期一周的课程，在 19 年来已举办过 20 次，重点关注在小鼠中进行葡萄糖钳夹所必需的外科和实验技术。该核心支持范德比尔特活力计划，为代表性不足的调查人员提供必要的时间和资源人群或机构以及早期职业调查人员。总之，核心促进糖尿病、肥胖、和新陈代谢研究，为研究人员提供很少有其他机构可行的新颖服务范德比尔特之外。