Gnotobiology Core

知识生物学核心

• 批准号: 10227104
• 负责人: JUDITH S EISEN
• 金额: $ 29.32万
• 依托单位: UNIVERSITY OF OREGON
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-06 至 2023-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10227104
• 关键词: Adolescent; Adult; Animals; Bacteria; Collection; Derivation procedure; Engineering; Ensure; Generations; Genetic Engineering; Germ-Free; Gnotobiotic; Health; Housing; Individual; Knowledge; Larva; Life; Methodology; Methods; Microbe; Mission; Modeling; Program Research Project Grants; Quality Control; Research; Research Project Grants; Science; System; Time; Vertebrates; Zebrafish; bacterial genetics; design; experimental study; genetic manipulation; improved; innovation; microbial; microbiota; success; transmission process

PROJECT SUMMARY/ABSTRACT (GNOTOBIOLOGY CORE) Gnotobiology (meaning “known life”) is the science of animals that are devoid of microbes (germ-free) or associated with defined microbial consortia. Experiments involving gnotobiotic zebrafish are central to each of the Research Projects of this Program Project Grant. The Gnotobiology Core provides the expertise necessary to ensure that experiments described in this proposal are carried out with maximal efficiency and minimal unnecessary use of vertebrate animals. Beyond ensuring that established methods of germ-free derivation and gnotobiotic experimentation are implemented to guarantee the highest level of quality control, the Gnotobiology Core will carry out research that extends the limits of our current gnotobiotic husbandry methodologies. These research objectives include extending the period of time over which germ-free zebrafish can be maintained, developing new ways to co-house zebrafish to control the inter-individual transmission of their microbes, and increasing the range and type of experiments that can be performed with zebrafish- associated microbes through developing and implementing new genetic engineering strategies in bacteria. Achieving these objectives will significantly enhance the ability to carry out experiments described in the Research Projects.

项目摘要/摘要（Gnotobiology Core） gnotobiology（意思是“已知生活”）是没有微生物（无菌）或 与定义的微生物联盟有关。涉及Gnotobiotic斑马鱼的实验对于每一个都是核心 该计划项目赠款的研究项目。 Gnotobiology Core提供了必要的专业知识 为了确保以最大效率和最低限度进行本提案中描述的实验 不必要使用脊椎动物。除了确保确定的无菌衍生方法和 实施gnotobiotic实验，以确保最高水平的质量控制， Gnotobiology Core将进行研究，以扩大我们目前的Gnotobiotic饲养的限制 方法。这些研究目标包括延长无菌斑马鱼的时间 可以维护，开发新的方法来共同建筑斑马鱼，以控制个人间的传播 它们的微生物，并增加可以用斑马鱼进行的实验范围和类型 通过开发和实施细菌中的新基因工程策略，相关的微生物。 实现这些目标将显着提高执行在 研究项目。