Flow & Image Cytometry Shared Resource

流动

• 批准号: 10398049
• 负责人: Kai Fu
• 金额: $ 21.33万
• 依托单位: ROSWELL PARK CANCER INSTITUTE CORP
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-06-16 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10398049
• 关键词: 1-Phosphatidylinositol 3-Kinase; Address; Advisory Committees; Agonist; Area; Award; Biological Assay; Buffaloes; CD80 gene; CD86 gene; CXCL12 gene; CXCR4 gene; Cancer Center Support Grant; Categories; Cells; Clinical; Collaborations; Confocal Microscopy; Consult; Consultations; Custom; Cytometry; Data Analyses; Detection; Development; Devices; Environment; Equipment; Experimental Designs; Fees; Flow Cytometry; Funding; Future; Gene Expression Profiling; Genomics Shared Resource; Goals; Guidelines; Human Resources; IFN consensus sequence binding protein; Image; Image Cytometry; Individual; Infrastructure; Institutes; Interleukin-6; L-Selectin; Leadership; Liver; Methodology; Microscope; Mission; Monitor; Multiple Myeloma; Myeloid-derived suppressor cells; NF-kappa B; NOTCH1 gene; Nature; Peer Review; Phenotype; Preparation; RNA; Radiation Protection; Reaction; Recommendation; Regulation; Reporting; Research; Research Personnel; Residual Neoplasm; Resolution; Resource Sharing; Resources; Role; Sampling; Secure; Services; Signal Transduction; Sorting - Cell Movement; Source; Specialist; Stains; Strategic Planning; Surveys; T-Cell Activation; T-Lymphocyte; TLR5 gene; Techniques; Technology; Testing; Transplant Recipients; United States National Institutes of Health; adaptive immunity; analytical tool; anti-cancer; anticancer research; antigen-specific T cells; base; cost effective; cost effectiveness; data acquisition; exosome; experimental study; graduate student; high dimensionality; imaging system; indexing; instrument; instrumentation; live cell imaging; lymph nodes; member; method development; nanoparticle; new technology; novel; oncolytic virotherapy; programs; quality assurance; remote control; tumor; user-friendly

PROJECT SUMMARY / ABSTRACT The mission of the Flow and Image Cytometry Shared Resource (FICSR) is to provide contemporary advanced flow cytometric analysis and sorting services, as well as imaging services at cellular and subcellular levels of resolution in a cost-effective, user-friendly and scientifically rigorous environment. A major strategic goal of the resource is to provide custom methods development services in a variety of labor-intensive, highly-specialized techniques. Development of methodology is often empirical in nature and cannot be easily obtained from outside sources. During the reporting period, FICSR has acquired new instrumentation, increased capacity, established new services, expanded educational programs, and engaged the Shared Resource Advisory Committee to develop a strategic plan that will enable us to continue to meet the rapidly evolving research needs of CCSG members. FICSR served a total of 134 Roswell users, of which 111 (93%) were CCSG members. There are three types of research investigators who use the FICSR. Level 1 users are those who are proficient in the techniques and know the instruments’ capabilities. For these investigators, the majority of our user base, the resource provides oversight and consultation. Level 2 users depend exclusively on the expertise of the resource staff to provide sample preparation, data acquisition, data analysis and its interpretation. Level 3 users include investigators who are new to flow and/or image cytometry or who are just beginning to consider new applications of this technology to address their research questions. Here the resource staff may perform pilot experiments to determine what would be a useful approach. The Specific Aims of the FICSR are: 1) To provide Roswell Park CCSG members access to cost-effective flow and image cytometry technology which is maintained according to a quality assurance program that follows regulatory agency guidelines and which meets the functional demands of their research programs; 2) To educate Roswell Park CCSG members on new developments in flow and image cytometry technology, their application and implementation pertaining to their specific research areas; 3) To enhance inter- and intra-programmatic collaborations by providing an educational and user-friendly environment in which FICSR personnel assist users with experimental design, execution, and analysis of their experiments commensurate with the user’s level of proficiency in the field. The resource offers services not provided by any other facility in the Buffalo region and the fees are among the lowest in the nation. The main objective of the FICSR is to stay on pace or ideally ahead of the cytometry needs of the CCSG user base. Thus, the resource leadership will continue close interactions with CCSG members to determine their needs and will also monitor the emergence of new technologies and analytic tools.

项目摘要 /摘要 流量和图像细胞仪共享资源（FICSR）的使命是提供当代高级 流式细胞仪分析和分类服务，以及在细胞和亚细胞水平的成像服务 在经济高效，用户友好和科学严格的环境中解决。一个主要的战略目标 资源是在各种劳动密集型，高度专业化的地方提供自定义方法开发服务 技术。方法论的发展本质上通常是经验的，不能轻易从 外部来源。在报告期间，FICSR获得了新的工具，增加的能力， 建立了新服务，扩大教育计划并参与共享资源咨询 制定战略计划的委员会，将使我们能够继续满足迅速发展的研究 CCSG成员的需求。 FICSR总共服务了134个罗斯威尔用户，其中111（93％）为CCSG 成员。使用FICSR的研究人员有三种类型的研究人员。 1级用户是那些 精通这些技术并了解工具的功能。对于这些调查人员，大多数 我们的用户群，资源提供了监督和咨询。 2级用户仅取决于 资源人员的专业知识，以提供样本准备，数据获取，数据分析及其 解释。 3级用户包括新手和/或图像细胞仪或仅仅是的调查人员 开始考虑该技术的新应用来解决他们的研究问题。在这里 资源人员可以执行试点实验，以确定什么是有用的方法。具体目标 FICSR的内容是：1）为Roswell Park CCSG成员提供具有成本效益的流程和图像 根据监管的质量保证计划维护的细胞仪技术 代理指南及满足其研究计划的功能要求； 2）教育罗斯威尔 Park CCSG成员在流动和图像细胞仪技术方面的新发展，其应用和 与其特定研究领域有关的实施； 3）增强媒介间和截面 通过提供教育和用户友好的环境来合作，FICSR人员协助 实验设计，执行和分析的用户与用户的实验相称 该领域的水平水平。该资源提供的服务不提供布法罗的任何其他设施 地区和费用是美国最低的。 FICSR的主要目的是留在太空或 理想情况下，在CCSG用户群的细胞仪需求之前。这，资源领导将继续关闭 与CCSG成员的互动以确定其需求，还将监视新的出现 技术和分析工具。