Flow & Image Cytometry Shared Resource

流动

基本信息

批准号：
10398049
负责人：
Kai Fu
金额：
$ 21.33万
依托单位：
ROSWELL PARK CANCER INSTITUTE CORP
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-06-16 至 2024-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10398049
关键词：
1-Phosphatidylinositol 3-Kinase Address Advisory Committees Agonist Area Award Biological Assay Buffaloes CD80 gene CD86 gene CXCL12 gene CXCR4 gene Cancer Center Support Grant Categories Cells Clinical Collaborations Confocal Microscopy Consult Consultations Custom Cytometry Data Analyses Detection Development Devices Environment Equipment Experimental Designs Fees Flow Cytometry Funding Future Gene Expression Profiling Genomics Shared Resource Goals Guidelines Human Resources IFN consensus sequence binding protein Image Image Cytometry Individual Infrastructure Institutes Interleukin-6 L-Selectin Leadership Liver Methodology Microscope Mission Monitor Multiple Myeloma Myeloid-derived suppressor cells NF-kappa B NOTCH1 gene Nature Peer Review Phenotype Preparation RNA Radiation Protection Reaction Recommendation Regulation Reporting Research Research Personnel Residual Neoplasm Resolution Resource Sharing Resources Role Sampling Secure Services Signal Transduction Sorting - Cell Movement Source Specialist Stains Strategic Planning Surveys T-Cell Activation T-Lymphocyte TLR5 gene Techniques Technology Testing Transplant Recipients United States National Institutes of Health adaptive immunity analytical tool anti-cancer anticancer research antigen-specific T cells base cost effective cost effectiveness data acquisition exosome experimental study graduate student high dimensionality imaging system indexing instrument instrumentation live cell imaging lymph nodes member method development nanoparticle new technology novel oncolytic virotherapy programs quality assurance remote control tumor user-friendly

项目摘要

PROJECT SUMMARY / ABSTRACT The mission of the Flow and Image Cytometry Shared Resource (FICSR) is to provide contemporary advanced flow cytometric analysis and sorting services, as well as imaging services at cellular and subcellular levels of resolution in a cost-effective, user-friendly and scientifically rigorous environment. A major strategic goal of the resource is to provide custom methods development services in a variety of labor-intensive, highly-specialized techniques. Development of methodology is often empirical in nature and cannot be easily obtained from outside sources. During the reporting period, FICSR has acquired new instrumentation, increased capacity, established new services, expanded educational programs, and engaged the Shared Resource Advisory Committee to develop a strategic plan that will enable us to continue to meet the rapidly evolving research needs of CCSG members. FICSR served a total of 134 Roswell users, of which 111 (93%) were CCSG members. There are three types of research investigators who use the FICSR. Level 1 users are those who are proficient in the techniques and know the instruments’ capabilities. For these investigators, the majority of our user base, the resource provides oversight and consultation. Level 2 users depend exclusively on the expertise of the resource staff to provide sample preparation, data acquisition, data analysis and its interpretation. Level 3 users include investigators who are new to flow and/or image cytometry or who are just beginning to consider new applications of this technology to address their research questions. Here the resource staff may perform pilot experiments to determine what would be a useful approach. The Specific Aims of the FICSR are: 1) To provide Roswell Park CCSG members access to cost-effective flow and image cytometry technology which is maintained according to a quality assurance program that follows regulatory agency guidelines and which meets the functional demands of their research programs; 2) To educate Roswell Park CCSG members on new developments in flow and image cytometry technology, their application and implementation pertaining to their specific research areas; 3) To enhance inter- and intra-programmatic collaborations by providing an educational and user-friendly environment in which FICSR personnel assist users with experimental design, execution, and analysis of their experiments commensurate with the user’s level of proficiency in the field. The resource offers services not provided by any other facility in the Buffalo region and the fees are among the lowest in the nation. The main objective of the FICSR is to stay on pace or ideally ahead of the cytometry needs of the CCSG user base. Thus, the resource leadership will continue close interactions with CCSG members to determine their needs and will also monitor the emergence of new technologies and analytic tools.

项目概要/摘要流式细胞术和图像细胞术共享资源 (FICSR) 的使命是提供当代先进的流式细胞术分析和分选服务，以及细胞和亚细胞水平的成像服务在具有成本效益、用户友好且科学严谨的环境中解决问题是该组织的主要战略目标。资源是提供各种劳动密集型、高度专业化的定制方法开发服务方法论的发展本质上通常是经验性的，并且不能轻易地从其中获得。外部来源在报告期内，FICSR 购买了新仪器，提高了能力，建立新的服务，扩大教育计划，并参与共享资源咨询委员会制定战略计划，使我们能够继续满足快速发展的研究 FICSR 总共服务了 134 名 Roswell 用户，其中 111 名（93%）是 CCSG 成员。使用 FICSR 的研究人员分为三类。对于这些调查人员来说，大多数人精通技术并了解仪器的功能。我们的用户群，该资源提供的监督和咨询完全依赖于 2 级用户。资源人员的专业知识提供样品制备、数据采集、数据分析及其 3 级用户包括刚接触流式细胞术和/或图像细胞术的研究人员。开始考虑这项技术的新应用来解决他们的研究问题。资源人员可以进行试点实验以确定什么是有用的方法。 FICSR 的目标是： 1) 为 Roswell Park CCSG 成员提供具有成本效益的流量和图像根据遵循监管的质量保证计划进行维护的细胞计数技术机构指南并满足其研究项目的功能需求；2) 教育罗斯威尔； Park CCSG 成员介绍流式细胞仪和图像细胞仪技术的新发展及其应用和 3) 加强计划间和计划内的实施；通过提供教育和用户友好的环境来促进合作，FICSR 人员可以在其中提供协助用户的实验设计、执行和实验分析与用户的该资源提供布法罗任何其他设施无法提供的服务。 FICSR 的主要目标是保持步伐或保持在全国最低水平。最好先于 CCSG 用户群的细胞计数需求，因此，资源领导地位将继续密切。与 CCSG 成员互动以确定他们的需求，并将监控新出现的情况技术和分析工具。