Molecular Histopathology Analytical Microscopy Core (MHAM)

分子组织病理学分析显微镜核心 (MHAM)

基本信息

批准号：
10223345
负责人：
Luis Del Valle
金额：
$ 3.27万
依托单位：
LSU HEALTH SCIENCES CENTER
依托单位国家：
美国
项目类别：
财政年份：
2017
资助国家：
美国
起止时间：
2017-08-15 至 2022-07-14
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10223345
关键词：
Animals Antibodies Applications Grants Archives Autopsy Biopsy Specimen Cell Separation Cell model Cell surface Cells Cellular Structures Centers of Research Excellence Clinical Collaborations Comet Assay Confocal Microscopy DNA DNA Probes Data Detection Evaluation Extramural Activities Fluorescent in Situ Hybridization Funding Gene Proteins Generations Goals Grant HIV Histologic Histological Techniques Histology Histopathology Human Image Immunofluorescence Immunologic Immunohistochemistry In Situ In Situ Hybridization In Vitro Individual Label Ligation Louisiana Malignant Neoplasms Manuscripts Mentors Messenger RNA Methodology Methods Microscope Microscopic Microscopy Mission Molecular Mus NCI Center for Cancer Research Normal tissue morphology Oncology Pathologic Pathologist Pathology Pathway interactions Patients Phase Photography Protein Dynamics Proteins RNA RNA Probes RNA-Protein Interaction Reproducibility Research Research Activity Research Personnel Research Project Grants Research Support Sampling Services Students Technical Expertise Techniques Technology Time Tissue imaging Tissues Training Translational Research Viral Viral Proteins biobank cancer biomarkers cancer cell data submission design digital experimental study fluorescence microscope human disease human tissue imaging study laser capture microdissection macromolecule medical schools microscopic imaging nature center programs prospective protein expression tissue processing tumor virus related cancer

项目摘要

ABSTRACT The Molecular Histopathology and Analytical Microscopy Core (MHAM) was created to provide advanced histopathology and microscopy services to researchers and clinicians conducting research at LSUHSC, including promising junior investigators (PJIs) of the Center for Translational Viral Oncology (CTVO). The MHAM provides PJIs with experimental planning design and imaging studies on the histology of tissues, the expression of cell surface and intracellular macromolecules including viral proteins, using state-of-the-art bright field, and confocal microscopy, immunohistochemistry, immunohistofluorescence, in situ hybridization, and in situ proximity ligation (DuoLink). These multiple methods are also used to determine protein and mRNA levels, their subcellular localization, protein-protein and RNA-protein interactions in the cells and tissues. Given the translational nature of the CTVO, this core is led by a trained pathologist, Dr. Luis Del Valle who, in addition to offering imaging services, also provides histopathological information and evaluation on patient tissues. The MHAM, working in close collaboration with the new HIV/Clinical Tumor Biorepository (HCTB) core will provide PJI's with access to clinical samples that are crucial to investigate the molecular and cellular pathways in virus- induced cancers. Moreover, the MHAM core provides investigators with training on tissue processing and management and assists investigators develop the necessary imaging and data for the submission of manuscripts and grants.

抽象的分子组织病理学和分析显微镜核心 (MHAM) 的创建是为了提供先进的为在 LSUHSC 进行研究的研究人员和临床医生提供组织病理学和显微镜服务，包括转化病毒肿瘤学中心 (CTVO) 的有前途的初级研究员 (PJI)。这 MHAM 为 PJI 提供组织组织学的实验规划设计和成像研究，使用最先进的 Bright 表达细胞表面和细胞内大分子，包括病毒蛋白场和共聚焦显微镜、免疫组织化学、免疫组织荧光、原位杂交和原位邻近连接（DuoLink）。这些多种方法还用于测定蛋白质和 mRNA 水平，它们的亚细胞定位、细胞和组织中的蛋白质-蛋白质和RNA-蛋白质相互作用。鉴于 CTVO 的转化性质，该核心由训练有素的病理学家 Luis Del Valle 博士领导，他除了提供成像服务，还提供患者组织的组织病理学信息和评估。这 MHAM 与新的 HIV/临床肿瘤生物储存库 (HCTB) 核心密切合作，将提供 PJI 可以获得临床样本，这些样本对于研究病毒的分子和细胞途径至关重要诱发癌症。此外，MHAM 核心为研究人员提供组织处理和管理并协助调查人员开发必要的成像和数据以提交手稿和赠款。